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Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA

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Edwards, Ulrike, Rogall, Till, Blöcker, Helmut, Emde, Monica, Böttger, Erik C.·1989·Nucleic Acids Research

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Abstract

Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M.kansasii was determined and found to be 98.7% homologous to that of M.bovis BCG. This is the first report on a contiguous sequence information of an entire amplified gene spanning 1.5 kb without any subcloning procedures.

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Authors: Edwards, U., Rogall, T., Blöcker, H., Emde, M., & Böttger, E. C.
Venue: Nucleic Acids Research
Publisher: Oxford University Press (OUP)
Published: 1989
Volume: 17
Issue: 19
Pages: 7843-7853
ISSN: 0305-1048 (ISSN-L)
1362-4962
DOI: 10.1093/nar/17.19.7843
OpenAlex: W2091309425
Cited by: 2,724
References: 3
FWCI: 4.22
Type: journal article
Language: en
Open access: green

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Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA

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