Exercise increases MEF2‐ and GEF DNA‐binding activities in human skeletal muscle
Abstract
Overexpression of GLUT4 exclusively in skeletal muscle enhances insulin action and improves glucose homeostasis. Transgenic studies have discovered two regions on the GLUT4 promoter conserved across several species that are required for normal GLUT4 expression in skeletal muscle. These regions contain binding motifs for the myocyte enhancer factor 2 (MEF2) family and GLUT4 enhancer factor (GEF). A single bout of exercise increases both GLUT4 transcription and mRNA abundance; however, the molecular mechanisms mediating this response remain largely unexplored. Thus, the aim of this study was to determine whether a single, acute bout of exercise increased the DNA-binding activities of MEF2 and GEF in human skeletal muscle. Seven subjects performed 60 min of cycling at approximately 70% of VO2peak. After exercise, the DNA-binding activities of both the MEF2A/D heterodimer and GEF were increased (P<0.05). There was no change in nuclear MEF2D or GEF abundance after exercise, but nuclear MEF2A abundance was increased (P<0.05). These data demonstrate that exercise increases MEF2 and GEF DNA binding and imply that these transcription factors could be potential targets for modulating GLUT4 expression in human skeletal muscle.
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AMPK can translocate to the nucleus where it regulates histone deacetylases, transcription factors and transcriptional co-activators such as HDAC5, MEF2 and PGC-1α.
“In a feed forward fashion AMPK can translocate to the nucleus [24,27] where it regulates Histone de-aceytlases, transcription factors and transcriptional co-activators such as histone deacetylase 5 (HDAC5), myocyte enhancing factor 2 (MEF2) and peroxisome proliferator activated receptor-γ co-activator-1α (PGC-1α) respectively (Figure 1) [28][29][30].”
From New strategies in sport nutrition to increase exercise performance (2016) · cites this paper as [29]
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- Authors
- McGee, S. L., Sparling, D., Olson, A., & Hargreaves, M.
- Affiliations
- Deakin University (AU)
- University of Oklahoma Health Sciences Center (US)
- Venue
- The FASEB Journal
- Publisher
- Wiley
- Published
- 2005-12-20
- Volume
- 20
- Issue
- 2
- Pages
- 348-349
- ISSN
- 0892-6638 (ISSN-L)
- 1530-6860
- OpenAlex
- W2146283921
- Cited by
- 100
- References
- 28
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 6 · 2019: 4 · 2018: 1 · 2017: 6 · 2016: 5 · 2015: 9 · 2014: 8 · 2013: 6 · 2012: 9
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The FASEB Journal (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (DK-062341)
- Diabetes Australia Research Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- physical activity, MEF2, GLUT4, skeletal muscle, DNA-binding activity, transcription factors, MEF2D, insulin sensitivity, glucose homeostasis, cycling exercise
- MeSH terms
- Adult, DNA, DNA-Binding Proteins, Gene Expression Regulation, Humans, Male, Promoter Regions, Genetic, Transcription Factors, Exercise, Myogenic Regulatory Factors, Muscle, Skeletal, Glucose Transporter Type 4, MEF2 Transcription Factors
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