Axon- or dendrite-predominant outgrowth induced by constituents from Ashwagandha
Abstract
We previously reported that the methanol extract of Ashwagandha (roots of Dunal) induced dendrite extension in a human neuroblastoma cell line. In this study, we found that six of the 18 compounds isolated from the methanol extract enhanced neurite outgrowth in human neuroblastoma SH-SY5Y cells. Double immunostaining was performed in rat cortical neurons using antibodies to phosphorylated NF-H as an axonal marker, and to MAP2 as a dendritic marker. In withanolide A-treated cells, the length of NF-H-positive processes was significantly increased compared with vehicle-treated cells, whereas, the length of MAP2-positive processes was increased by withanosides IV and VI. These results suggest that axons are predominantly extended by withanolide A, and dendrites by withanosides IV and VI.
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Aqueous extracts of Withania somnifera elevated the activity of choline acetyl transferase and acetylcholine in rats.
“Subsequently, aqueous extracts of W. somnifera elevated the activity of choline acetyl transferase and acetylcholine in rats, potentially explicating the memory- and cognition-enhancing effects [87,90].”
From The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment (2022) · cites this paper as [87]
Therapeutic treatments consisting of Withania somnifera methanol extractions trigger the outgrowth of neurites in a time- and dose-dependent approach in human neuroblastoma cells.
“Consequently, therapeutic treatments that consist of W. somnifera’s methanol extractions have been shown to trigger the outgrowth of neurites in a time- and dose-dependent approach in human neuroblastoma cells [87].”
From The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment (2022) · cites this paper as [87]
Elevated choline acetyl transferase and acetylcholine activity potentially explains the memory- and cognition-enhancing effects of Withania somnifera aqueous extracts.
“Subsequently, aqueous extracts of W. somnifera elevated the activity of choline acetyl transferase and acetylcholine in rats, potentially explicating the memory- and cognition-enhancing effects [87,90].”
From The Multifaceted Role of Neuroprotective Plants in Alzheimer’s Disease Treatment (2022) · cites this paper as [87]
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“somnifera’s methanol extractions have been shown to trigger the outgrowth of neurites in a time- and dose-dependent approach in human neuroblastoma cells [87].”
Background
- Authors
- Kuboyama, T., Tohda, C., Zhao, J., Nakamura, N., Hattori, M., & Komatsu, K.
- Affiliations
- Institute of Natural Medicine, University of Toyama (JP)
- Venue
- Neuroreport
- Published
- 2002-10
- Volume
- 13
- Issue
- 14
- Pages
- 1715-1720
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W2012697226
- Cited by
- 146
- References
- 22
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 9 · 2023: 8 · 2022: 7 · 2021: 8 · 2020: 10 · 2019: 3 · 2018: 5 · 2017: 9 · 2016: 10 · 2015: 11 · 2014: 8 · 2013: 9 · 2012: 3
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- Withania somnifera, neurite outgrowth, axon growth, withanolide A, neuroblastoma cells, SH-SY5Y cells, MAP2, cortical neurons
- MeSH terms
- Animals, Axons, Cell Differentiation, Cells, Cultured, Cerebral Cortex, Dendrites, Female, Fetus, Growth Substances, Humans, Immunohistochemistry, Microtubule-Associated Proteins, Plant Extracts, Pregnancy, Tumor Cells, Cultured, Molecular Structure, Neurites, Neurofilament Proteins, Rats, Sprague-Dawley, Withania, Rats
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