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Cited by 111Neuroscience and Neuropharmacology ResearchNeuroinflammation and Neurodegeneration MechanismsMedical Imaging Techniques and Applications

Selective Uptake of [ 14 C]2-Deoxyglucose by Neurons and Astrocytes: High-Resolution Microautoradiographic Imaging by Cellular 14 C-Trajectography Combined with Immunohistochemistry

Nehlig, Astrid, Wittendorp-Rechenmann, Elisabeth, Dao Lam, Chang·2004·Journal of Cerebral Blood Flow & Metabolism
DOI 10.1097/01.wcb.0000128533.84196.d8
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Abstract

At the moment, there is no direct in vivo evidence of the relative amount of glucose taken up and metabolized by glial cells and neurons, respectively. Therefore, we developed a specific high cellular resolution β-trajectory approach that allows recording and identification of individual tracks of electrons emitted during disintegrations of 14 C. We used [ 14 C]2-deoxyglucose (2DG), which is an analog of glucose and is not metabolized further than the first phosphorylation by hexokinase; this property allows localization of the tracer within the cell type where it is phosphorylated. The present technical approach associated a method of cellular trajectography mainly characterized by the high thickness of the emulsion (15 μm), which permits following of the trajectory of individual electrons. This technique was improved to preserve the in vivo label of diffusible compounds such as 2DG and 2DG-6P and associated with immunohistochemical detection of neurons and astrocytes. β-Track counting of labeled compounds was performed in 5 μm glial fibrillary acidic protein (GFAP)- and microtubule-associated protein (MAP)2-immunolabeled paraffin adjacent sections. Of 3,075 counted β-tracks, 53.0% were localized in astrocytes on GFAP-labeled sections and 60.1% in neurons on MAP2-labeled sections. These data represent the first in vivo evidence of the compartmentation of uptake and metabolism of glucose in neurons and astrocytes.

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  • Neurons are capable of directly taking up glucose.

    “Nevertheless, as extracellular glucose concentrations can be substantial, neurons are also capable of directly taking up glucose10.”

    From Energy paradox in REM sleep: balancing supply and consumption in brain metabolism (2026) · cites this paper as [10]

  • In awake but unstimulated rats, [14C]-2-DG uptake is roughly comparable in neurons and astrocytes.

    “High-resolution autoradiographic analysis of [14C]–2-DG uptake in awake but unstimulated rats revealed roughly comparable label uptake in neurons and astrocytes (Nehlig et al., 2004); another in vivo labeling study using the fluorescent glucose analogue 2-NBDG showed stronger signals in neurons (Itoh et al., 2004).”

    From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Nehlig et al., 2004

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Authors
Nehlig, A., Wittendorp-Rechenmann, E., & Dao Lam, C.
Affiliations
  • Inserm (FR)
  • INSERM U 398, University Louis Pasteur, Faculty of Medicine, Strasbourg, France
Corresponding author
Nehlig, Astrid
ORCID
Nehlig, Astrid
Venue
Journal of Cerebral Blood Flow & Metabolism
Publisher
SAGE Publications
Published
2004-09
Volume
24
Issue
9
Pages
1004-1014
ISSN
  • 0271-678X (ISSN-L)
  • 1559-7016
DOI
10.1097/01.wcb.0000128533.84196.d8
OpenAlex
W1975262997
Cited by
111
References
73
Citations by year
2026: 3 · 2025: 3 · 2024: 6 · 2023: 2 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 5 · 2018: 5 · 2017: 3 · 2016: 8 · 2015: 9 · 2014: 5 · 2013: 3 · 2012: 9
FWCI
1.99
Type
journal article
Language
en
Open access
closed
Licence
journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations
  • Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Medical Imaging Techniques and Applications
Keywords
2-deoxyglucose, glucose uptake, neurons, astrocytes, microautoradiography, hexokinase, glucose metabolism, glial fibrillary acidic protein, MAP2
MeSH terms
Animals, Astrocytes, Autoradiography, Brain, Carbon Radioisotopes, Deoxyglucose, Diagnostic Imaging, Glial Fibrillary Acidic Protein, Immunohistochemistry, Male, Microtubule-Associated Proteins, Neurons, Rats, Sprague-Dawley, Rats

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