Cited by 147Ruminant Nutrition and Digestive PhysiologyAnaerobic Digestion and Biogas ProductionGut microbiota and health

Study of methanogen communities associated with different rumen protozoal populations

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Belanche, Alejandro, de la Fuente, Gabriel, Newbold, Charles J.·2014·FEMS Microbiology Ecology

Abstract

Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7-3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67% similarity within treatment) in the rumen with similar diversity but different structures than PAM (P < 0.05). On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P < 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.

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Research works printed but not linked: 3

  1. Belanche A de la Fuente G Moorby JM Newbold CJ Are methane emissions determined by the fermentation pattern or by the rumen microbiota? Proceedings in: Gut microbiota: Friend or Foe? 2012b France Clermont-Ferrand 27 (INRA-RRI, ED)
  2. Coleman GS Mcdonald IW Warner ACI The interrelationship between rumen ciliate protozoa and bacteria Proceedings of the International Symposium on Ruminant Physiology 1975 Armidale, NSW University of New England Publishing Unit 602
  3. Dehority BA Laboratory Manual for Classification and Morphology of Ruminal Ciliate Protozoa 1993 Boca Raton, FL CRC Press, Inc 120

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Authors
Belanche, A., de la Fuente, G., & Newbold, C. J.
Affiliations
  • Aberystwyth University (GB)
  • Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK
Corresponding author
Newbold, Charles J.
Venue
FEMS Microbiology Ecology
Published
2014-12-01
Volume
90
Issue
3
Pages
663-677
ISSN
  • 0168-6496 (ISSN-L)
  • 1574-6941
OpenAlex
W2018339579
Cited by
147
References
60
Citations by year
2026: 6 · 2025: 16 · 2024: 20 · 2023: 11 · 2022: 11 · 2021: 19 · 2020: 12 · 2019: 16 · 2018: 10 · 2017: 7 · 2016: 10 · 2015: 8 · 2014: 1
FWCI
17.27
Type
journal article
Language
en
Open access
Locations
  • FEMS Microbiology Ecology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • RECERCAT (Consorci de Serveis Universitaris de Catalunya) (publishedVersion, cc-by-nc)
  • Repositori ObertUDL (University of Lleida) (publishedVersion, other-oa)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc)
Funders
European Commission (FP7, REDNEX project)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
rumen methanogenesis, rumen protozoa, endosymbiotic bacteria, methane mitigation, sheep
MeSH terms
Animals, Bacteria, Methane, Polymorphism, Restriction Fragment Length, Rumen, Symbiosis, Trichostomatida, Euryarchaeota, Sheep, Domestic

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