The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
PHYTOPLANKTON LIPIDS: INTERSPECIFIC DIFFERENCES AND EFFECTS OF NITRATE, SILICATE AND LIGHT‐DARK CYCLES1
Abstract
ABSTRACT The lipid content of various phytoplankton species was measured in response to nitrogen and silicon limitation and over the cell cycle in synchronized cultures. In a survey of 30 species it was found that during log‐phase growth, green algae contained an average of 17.1% total lipids (% of total dry weight), whereas diatoms contained an average of 24.5%. Nitrogen deprivation for 4 to 9 days resulted in 2‐ to 3‐fold increases in the lipid content of green algae, whereas both increases and decreases were noted in diatoms, depending on the species. The greatest lipid content measured in the study was 72% in Monallantus salina (strain GSB Sticho) which had been deprived of nitrogen for 9 days. Nitrate replenishment in a nitrogen starved culture of Oocystis polymorpha Groover & Bold showed that the excess cellular lipids do not rapidly disappear during recovery, until cell division occurs. A silicate deprivation experiment with Cyclotella cryptica Reimann, Lewin & Guillard (strain 7c) showed an increase in the total cellular lipid fraction from. 30 to 42% of dry weight within 6 h of the onset of silicon limitation, while the mass of lipid material per cell doubled within 12 h. The total lipid fraction in O. polymorpha was found to remain constant over the cell cycle in synchronized cultures regardless of the light regime. The data presented provided the first internally consistent study of phytoplankton lipids for a wide range of species and several growth conditions.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The relative composition of algal lipids depends greatly on the species used and the nutrient, environmental and developmental conditions in which the cells are cultured and harvested [11][12][13][14].”
From Review of Methods Used for Microalgal Lipid-Content Analysis (2011) · cites this paper as [11]
Citations
Cites
A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
A practical hand-book of seawater analysis
Strickland, J., & PARSONS, T. R. (1968) · Helmholtz-Zentrum für Polar-und Meeresforschung (Alfred-Wegener-Institut) · Cited by 10,489
Culture of Phytoplankton for Feeding Marine Invertebrates
Guillard, R. R. L. (1975) · Springer eBooks · doi:10.1007/978-1-4615-8714-9_3 · Cited by 5,478
THE CULTURE COLLEVTION OF ALGAE AT THE UNIVERSITY OF TEXAS ATAUSTIN123
Starr, R. C. (1978) · Journal of Phycology · doi:10.1111/j.1529-8817.1978.tb02507.x · Cited by 832
Algal Cultures and Phytoplankton Ecology.
Lund, J., & Fogg, G. E. (1965) · Journal of Ecology · doi:10.2307/2257641 · Cited by 758
THE CHEMICAL COMPOSITION OF CHLORELLA; EFFECT OF ENVIRONMENTAL CONDITIONS
Spoehr, H. A., & Milner, H. W. (1949) · PLANT PHYSIOLOGY · doi:10.1104/pp.24.1.120 · Cited by 427
Nitrogenous Nutrition of Marine Phytoplankton in Nutrient-Depleted Waters
McCarthy, J. J., & Goldman, J. C. (1979) · Science · doi:10.1126/science.203.4381.670 · Cited by 422
Kates, M., & Volcani, B. E. (1966) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(66)90009-9 · Cited by 360
Caperon, J., & Meyer, J. L. (1972) · Deep Sea Research and Oceanographic Abstracts · doi:10.1016/0011-7471(72)90089-7 · Cited by 297
Biochemistry of Silicon and Related Problems
(1978) · Springer eBooks · doi:10.1007/978-1-4613-4018-8 · Cited by 208
Research works printed but not linked: 10
- Fogg G. E., 1959, Nitrogen nutrition and metabolic patterns in algae, Symp. Soc. Exp. Biol., 13, 106
- Gons H. J., 1979, Growth of Scenedesmus protuberans Fritsch in light‐limited continuous cultures with a light‐dark cycle, Arch. Hydrobiol., 85, 41
- Klyachko‐Gurvich G. L., 1967, Comparative characteristics of the growth and direction of biosynthesis of various strains of Chlorella under nitrogen deficiency conditions. III. Synthesis of fatty acids, sov. Plant Physiol. (Engl. Transl. Fiziol. Rast.), 16, 205
- McKnight D. M., 1979, Interactions between Freshwater Plankton and Copper Speciation, 283
- Orcutt D. M., 1973, Lipid and elemental Composition of diatoms grown in chemically defined media, J. Phycol., 9, 13
- Pohl P., 1974, Control of unsaturated fatty acid biosynthesis in unicellular algae by the nitrogen content of the nutrient medium and by the wavelength of light, J. Am. Oil Chem. Soc., 51, 521 A
- Shifrin N. S.1980.Phytoplankton Lipids: Environmental Influences on Production and Possible Commercial Applications Ph.D. thesis Massachusetts Institute of Technology Cambridge Massachusetts 297pp.
- Shifrin N. S., 1980, Algae Biomass, 627
- Werner D., 1977, The Biology of the Diatoms, 498
- Zhukova T. S., 1969, Comparative characteristics of the growth and direction of biosynthesis of various strains of Chlorella under conditions of nitrogen starvation. II. Formation of carbohydrates and lipids, Sov. Plant Physiol. (Engl. Transl. Fiziol. Rast.), 16, 96
Cited by
Sorted by citations
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Rodolfi, L., Chini Zittelli, G., Bassi, N., Padovani, G., Biondi, N., Bonini, G., & Tredici, M. R. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22033 · Cited by 2,879
Redfield revisited: Variability of C:N: P in marine microalgae and its biochemical basis
Geider, R., & La Roche, J. (2002) · European Journal of Phycology · doi:10.1017/s0967026201003456 · Cited by 1,412
Lipid productivity as a key characteristic for choosing algal species for biodiesel production
Griffiths, M. J., & Harrison, S. T. L. (2009) · Journal of Applied Phycology · doi:10.1007/s10811-008-9392-7 · Cited by 1,421
Williams, P. J. L. B., & Laurens, L. M. L. (2010) · Energy & Environmental Science · doi:10.1039/b924978h · Cited by 1,223
Increase in Chlorella strains calorific values when grown in low nitrogen medium
Illman, A. M., Scragg, A. H., & Shales, S. W. (2000) · Enzyme and Microbial Technology · doi:10.1016/s0141-0229(00)00266-0 · Cited by 1,008
Juneja, A., Ceballos, R., & Murthy, G. (2013) · Energies · doi:10.3390/en6094607 · Cited by 835
The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp.
Pal, D., Khozin-Goldberg, I., Cohen, Z., & Boussiba, S. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3170-1 · Cited by 575
Sajjadi, B., Chen, W.-Y., Raman, A. A. A., & Ibrahim, S. (2018) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2018.07.050 · Cited by 535
Fatty acids from 28 marine microalgae
Viso, A.-C., & Marty, J.-C. (1993) · Phytochemistry · doi:10.1016/s0031-9422(00)90839-2 · Cited by 400
Hydrothermal liquefaction for algal biorefinery: A critical review
Tian, C., Li, B., Liu, Z., Zhang, Y., & Lu, H. (2014) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.07.030 · Cited by 389
Yongmanitchai, W., & Ward, O. P. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.2.419-425.1991 · Cited by 371
Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red
Cooksey, K. E., Guckert, J. B., Williams, S. A., & Callis, P. R. (1987) · Journal of Microbiological Methods · doi:10.1016/0167-7012(87)90019-4 · Cited by 361
Solovchenko, A. E., Khozin-Goldberg, I., Didi-Cohen, S., Cohen, Z., & Merzlyak, M. N. (2008) · Journal of Applied Phycology · doi:10.1007/s10811-007-9233-0 · Cited by 354
Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L. (2012) · Journal of Applied Phycology · doi:10.1007/s10811-011-9723-y · Cited by 341
Fatty Acid Profiles and Production in Marine Phytoplankton
Jónasdóttir, S. H. (2019) · Marine Drugs · doi:10.3390/md17030151 · Cited by 321
Stehfest, K., Toepel, J., & Wilhelm, C. (2005) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2005.07.001 · Cited by 316
Factors Affecting Algae Biofilm Growth and Lipid Production: A Review
Schnurr, P. J., & Allen, D. G. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.07.090 · Cited by 276
Microalgal lipids: A review of lipids potential and quantification for 95 phytoplankton species
Morales, M., Aflalo, C., & Bernard, O. (2021) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2021.106108 · Cited by 273
Gardner, R., Peters, P., Peyton, B., & Cooksey, K. E. (2011) · Journal of Applied Phycology · doi:10.1007/s10811-010-9633-4 · Cited by 159
Two phase microalgae growth in the open system for enhanced lipid productivity
Das, P., Aziz, S. S., & Obbard, J. P. (2011) · Renewable Energy · doi:10.1016/j.renene.2011.02.002 · Cited by 144
The effect of nitrogen limitation on lipid productivity and cell composition in Chlorella vulgaris
Griffiths, M. J., van Hille, R. P., & Harrison, S. T. L. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5442-4 · Cited by 137
Wagner, H., Liu, Z., Langner, U., Stehfest, K., & Wilhelm, C. (2010) · Journal of Biophotonics · doi:10.1002/jbio.201000019 · Cited by 134
Mus, F., Toussaint, J.-P., Cooksey, K. E., Fields, M. W., Gerlach, R., Peyton, B. M., & Carlson, R. P. (2013) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-4747-7 · Cited by 132
Palmucci, M., Ratti, S., & Giordano, M. (2011) · Journal of Phycology · doi:10.1111/j.1529-8817.2011.00963.x · Cited by 104
Li, S., Ji, L., Chen, C., Zhao, S., Sun, M., Gao, Z., Wu, H., & Fan, J. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.123362 · Cited by 110
Pernet, F., Tremblay, R., Demers, E., & Roussy, M. (2003) · Aquaculture · doi:10.1016/s0044-8486(03)00030-9 · Cited by 104
Nutrient temperature and light stress alter phosphorus and carbon forms in culture-grown algae
Cade-Menun, B. J., & Paytan, A. (2010) · Marine Chemistry · doi:10.1016/j.marchem.2010.03.002 · Cited by 93
Kojima, E., & Zhang, K. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80158-3 · Cited by 76
Review of Methods Used for Microalgal Lipid-Content Analysis
Han, Y., Wen, Q., Chen, Z., & Li, P. (2011) · Energy Procedia · doi:10.1016/j.egypro.2011.10.124 · Cited by 65
Phadwal, K., & Singh, P. K. (2003) · Bioresource Technology · doi:10.1016/s0960-8524(03)00090-7 · Cited by 62
Vu, M. T. T., Douëtte, C., Rayner, T. A., Thoisen, C., Nielsen, S. L., & Hansen, B. W. (2016) · Journal of Applied Phycology · doi:10.1007/s10811-015-0722-2 · Cited by 59
Shrestha, N., Dandinpet, K. K., & Schneegurt, M. A. (2020) · Journal of Applied Phycology · doi:10.1007/s10811-020-02144-x · Cited by 31
Metadata
- Authors
- Shifrin, N. S., & Chisholm, S. W.
- Affiliations
- Massachusetts Institute of Technology (US)
- Corresponding author
- Chisholm, Sallie W.
- ORCID
- Chisholm, Sallie W.
- Venue
- Journal of Phycology
- Publisher
- Wiley
- Published
- 1981-12
- Volume
- 17
- Issue
- 4
- Pages
- 374-384
- ISSN
- 0022-3646 (ISSN-L)
- 1529-8817
- OpenAlex
- W2054891426
- Cited by
- 488
- References
- 45
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 5 · 2023: 4 · 2022: 3 · 2021: 15 · 2020: 19 · 2019: 13 · 2018: 15 · 2017: 16 · 2016: 18 · 2015: 19 · 2014: 32 · 2013: 35 · 2012: 30
- FWCI
- 8.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Phycology (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Plankton and Biogeochemistry
- Keywords
- nitrogen limitation, silicon limitation, diatoms, green algae, Cyclotella cryptica, lipid content, light-dark cycle, cell cycle, synchronous culture, nitrate recovery, silicon recovery
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
