Exercise induces interleukin‐8 receptor (CXCR2) expression in human skeletal muscle
Open accessAbstract
Exercise induces a marked increase in interleukin‐8 (IL‐8) mRNA and protein expression within skeletal muscle fibres. Interleukin‐8 belongs to a subfamily of CXC chemokines containing a Glu‐Leu‐Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo, and IL‐8 has been shown to act as an angiogenic factor in human microvascular endothelial cells by binding to the CXC receptor 2 (CXCR2). In the present study, we examined the expression of the interleukin‐8 receptor CXCR2 in human skeletal muscle biopsies after concentric exercise. Healthy volunteers were randomized to either 3 h of cycle ergometer exercise at 60% of maximum oxygen uptake (n= 8) or rest (n= 7). Muscle biopsy samples were obtained from the vastus lateralis before exercise (0 h), immediately after exercise (3 h), and at 4.5, 6, 9 and 24 h. Skeletal muscle CXCR2 mRNA increased significantly in response to exercise (3 and 4.5 h) when compared with pre‐exercise samples. Expression of the CXCR2 protein was low in skeletal muscle biopsies before exercise and at the end of the exercise period (3 h). However, at 4.5–9 h, an increase in CXCR2 protein was seen in the vascular endothelium, and also slightly within the muscle fibres, as determined by immunohistochemistry. The present study demonstrates that concentric exercise induces CXCR2 mRNA and protein expression in the vascular endothelial cells of the muscle fibres. These findings suggest that muscle‐derived IL‐8 may act locally to stimulate angiogenesis through CXCR2 receptor signalling.
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Muscle secretes VEGF, angiopoietin 1 and IL-8 to regulate tissue angiogenesis, modulate blood flow and increase nutrient availability to support tissue growth.
“muscle secretes vascular endothelial growth factor (VEGF)45,46, angiopoietin 1 (REF.47) and IL-8 (REF.48) to regulate tissue angiogenesis, modulate blood flow and increase nutrient availability to support tissue growth27,49–52”
From Exerkines in health, resilience and disease (2022) · cites this paper as [48]
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- Authors
- Frydelund‐Larsen, L., Penkowa, M., Akerstrom, T., Zankari, A., Nielsen, S., & Pedersen, B. K.
- Affiliations
- University of Copenhagen (DK)
- Venue
- Experimental Physiology
- Publisher
- Wiley
- Published
- 2007-01
- Volume
- 92
- Issue
- 1
- Pages
- 233-240
- ISSN
- 0958-0670 (ISSN-L)
- 1469-445X
- OpenAlex
- W1527466416
- Cited by
- 106
- References
- 55
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 3 · 2023: 7 · 2022: 4 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 3 · 2017: 6 · 2016: 7 · 2015: 5 · 2014: 3 · 2013: 5 · 2012: 7
- FWCI
- 7.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Experimental Physiology (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Funders
- National Research Foundation
- Danmarks Grundforskningsfond (02-512-555, DG 02–512‐555, LSHM-CT-2004-005272, 504–14, LSHM-CT-2004-005272 EXGENESIS)
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Oncology › Chemokine receptors and signaling
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- interleukin-8, CXCR2, skeletal muscle, physical activity, angiogenesis, CXC chemokines, vascular endothelium, vastus lateralis, muscle biopsy, mRNA expression, protein expression, cycle ergometry
- MeSH terms
- Endoglin, Adult, Endothelium, Vascular, Fluorescent Antibody Technique, Humans, Male, Receptors, Cell Surface, Reference Values, RNA, Messenger, Time Factors, Exercise, Antigens, CD, Up-Regulation, Muscle, Skeletal, Muscle Fibers, Skeletal, Reverse Transcriptase Polymerase Chain Reaction, Receptors, Interleukin-8B
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