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Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node
Abstract
Lymphocyte motility is vital for trafficking within lymphoid organs and for initiating contact with antigen-presenting cells. Visualization of these processes has previously been limited to in vitro systems. We describe the use of two-photon laser microscopy to image the dynamic behavior of individual living lymphocytes deep within intact lymph nodes. In their native environment, T cells achieved peak velocities of more than 25 micrometers per minute, displaying a motility coefficient that is five to six times that of B cells. Antigenic challenge changed T cell trajectories from random walks to “swarms” and stable clusters. Real-time two-photon imaging reveals lymphocyte behaviors that are fundamental to the initiation of the immune response.
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Metadata
- Authors
- Miller, M. J., Wei, S. H., Parker, I., & Cahalan, M. D.
- Venue
- Science
- Published
- 2002-06-07
- Volume
- 296
- Issue
- 5574
- Pages
- 1869-1873
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2149928606
- Cited by
- 1,207
- References
- 0
- FWCI
- 39.37
- Type
- journal article
- Language
- en
- Open access
- closed
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