Cited by 329Viral Infectious Diseases and Gene Expression in InsectsMicrobial infections and disease researchIdentification and Quantification in Food

The DOI matches its Crossref record; 103 of the 103 references it cites were found in a registry or an index.

Cross-Contamination of Cells in Culture

Nelson-Rees, W. A., Daniels, D. W., Flandermeyer, R. R.·1981·Science

DOI 10.1126/science.6451928

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Abstract

Lists are presented of references to all known publications describing cell properties that serve to characterize (i) known strains of HeLa and purported human cell lines indicted as HeLa contaminants, (ii) strains of human cell lines contaminated with human but non-HeLa cells, and (iii) strains of cells contaminated by cells from one or more other species. Frequencies of cell cross-contaminations are cited and references are presented to relatively simple techniques that could serve to detect such contamination.

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Crossref · 10.1126/science.6451928

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One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23
Fogh, J., Fogh, J. M., & Orfeo, T. (1977) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/59.1.221 · Cited by 1,599
Differentiated Rat Glial Cell Strain in Tissue Culture
Benda, P., Lightbody, J., SATO, G. H., Levine, L., & Sweet, W. H. (1968) · Science · doi:10.1126/science.161.3839.370 · Cited by 1,472
Untitled work
(1952) · Cancer Research · Cited by 987
Establishment of Four Strains of Cells from Insect Tissues Grown in vitro
Grace, T. D. C. (1962) · Nature · doi:10.1038/195788a0 · Cited by 866
The effects of androgens and antiandrogens on hormone-responsive human breast cancer in long-term tissue culture.
Lippman, M. E., Bolan, G., & Huff, K. K. (1976) · PubMed · Cited by 808
Absence of HeLa Cell Contamination in 169 Cell Lines Derived From Human Tumors2
Fogh, J., Wright, W. C., & Loveless, J. D. (1977) · JNCI: Journal of the National Cancer Institute · doi:10.1093/jnci/58.2.209 · Cited by 663
Immunofluorescence and Herpes-Type Virus Particles in the P3HR-1 Burkitt Lymphoma Cell Line
Hinuma, Y., Konn, M., Yamaguchi, J., Wudarski, D. J., Blakeslee, J. R., & Grace, J. T. (1967) · Journal of Virology · doi:10.1128/jvi.1.5.1045-1051.1967 · Cited by 493
Propagation in a Fluid Medium of a Human Epidermoid Carcinoma, Strain KB
Eagle, H. (1955) · Experimental Biology and Medicine · doi:10.3181/00379727-89-21811 · Cited by 483
Morphological and Virological Investigations on Cultured Burkitt Tumor Lymphoblasts (Strain Raji) 2
Epstein, M. A., Achong, B. G., Barr, Y. M., Zając, B., Henle, G., & Henle, W. (1966) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/37.4.547 · Cited by 386
A PERMANENT CELL LINE FROM THE FATHEAD MINNOW (PIMEPHALES PROMELAS)*
Gravell, M., & Malsberger, R. G. (1965) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1965.tb14302.x · Cited by 324

Show all 103 references

Research works printed but not linked: 38

ANDZHAPARIDZE, O.G., VOPROSY VIRUSOLOGII 6: 686 (1972).
ARATA, T, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 7: 242 (1972).
BYKOVSKY, A.F., VOPROSY VIRUSOLOGIIOGII 18: 215 (1973).
CHOU, J. Y., IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 12: 589 (1976).
CONNER, B. R., IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 16: 446 (1980).
CREASEY, A. A., IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 15: 342 (1979).
DAVIS, E. V., FEDERATION PROCEEDINGS 19: 386 (1960).
DEFENDI, V, JOURNAL OF THE NATIONAL CANCER INSTITUTE 25: 359 (1965).
EPSTEIN, M.A., CULTIVATION IN VITRO OF HUMAN LYMPHOBLASTS FROM BURKITTS MALIGNANT LYMPHOMA, LANCET 1: 252 (1964).
FANCKE, U, CHROMOSOMA 41: 111 (1973).
FOGH, J, HUMAN TUMOR CELLS IN: 115 (1975).
FOGH, J, REVIEW OF CELL CULTURE CONTAMINATIONS, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 7: 26 (1971).
FOGH, J, CORRECTION, JOURNAL OF THE NATIONAL CANCER INSTITUTE 59: 1035 (1977).
GARTLER, S. M., NATIONAL CANCER INSTITUTE MONOGRAPHS 26: 167 (1967).
GEY, G. O., CANCER RESEARCH 12: 264 (1952).
GREENE, A.E., SPECIES IDENTITY OF INSECT CELL LINES, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 7: 313 (1972).
HERRICK, P.R., SEROLOGIC AND KARYOLOGIC EVIDENCE OF INCORRECT IDENTITY OF AN ANIMAL CELL LINE (GUINEA PIG SPLEEN), IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 6: 143 (1970).
JONES, H.W., GEY,GO (1899-1970) - HELA CELL AND A REAPPRAISAL OF ITS ORIGIN, OBSTETRICS AND GYNECOLOGY 38: 945 (1971).
KULAGINA, O.E., VOPROSY VIRUSOLOGII 6: 677 (1976).
LAVAPPA, K.S., IN VITRO 10: 374 (1974).
LAVAPPA, K.S., SURVEY OF ATCC STOCKS OF HUMAN CELL LINES FOR HELA CONTAMINATION, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 14: 469 (1978).
LIGHTBODY, J, J NEUROBIOL 1: 411 (1970).
LIPPMAN, M, EFFECTS OF ESTROGENS AND ANTIESTROGENS ON HORMONE-RESPONSIVE HUMAN BREAST-CANCER IN LONG-TERM TISSUE-CULTURE, CANCER RESEARCH 36: 4595 (1976).
MAYYASI, S.A., ANTIGENIC CHARACTERISTICS OF RAUSCHER VIRUS CULTIVATED IN HUMAN EMBRYONIC KIDNEY CELLS, PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH 11: 54 (1970).
MELNICK, P.J., PROG HISTOCHEM CYTOC 2: 1 (1971).
MIKHAILOVA, G.R., CYTOLOGIA 19: 786 (1977).
MILLER, E.E., MALIGNANT CHARACTERISTICS OF A HUMAN BREAST TUMOR LINE AFTER LONG-TERM INVITRO CULTIVATION, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 12: 331 (1976).
NELSONREES, W.A., PROG CLIN BIOL RES 26: 25 (1978).
OBRIEN, S.J., A MOLECULAR APPROACH TO THE IDENTIFICATION AND INDIVIDUALIZATION OF HUMAN AND ANIMAL-CELLS IN CULTURE - ISOENZYME AND ALLOZYME GENETIC SIGNATURES, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 16: 119 (1980).
OHNUKI, Y, IN VITRO 5: 153 (1969).
OKADA, K, UROL RES 2: 111 (1974).
PRIORI, E.S., NATURE LONDON NEW BI 233: 61 (1971).
SEMAN, G, 2 CELL LINES (SH-2 AND SH-3) DERIVED FROM HUMAN BREAST-CANCER, PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH 16: 59 (1975).
STORY, M.T., IN VITRO 10: 388 (1974).
STULBERG, C.S., CONTAMINATION TISSUE: 1 (1973).
SYVERTON, J.T., CANCER RES 17: 23 (1957).
VANDERVEEN, J, ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG 81: 230 (1958).
WANG, H. C., IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 8: 443 (1973).

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Chalak, M., Hesaraki, M., Mirbahari, S. N., Yeganeh, M., Abdi, S., Rajabi, S., & Hemmatzadeh, F. (2024) · Life · doi:10.3390/life14030417 · Cited by 63
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Thacker, J., Webb, M. B. T., & Debenham, P. G. (1988) · Somatic Cell and Molecular Genetics · doi:10.1007/bf01535307 · Cited by 52
Cell culture quality control by rapid isoenzymatic characterization
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Nardone, R. M. (2008) · BioTechniques · doi:10.2144/000112925 · Cited by 50
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Methods for maintaining insect cell cultures.
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Attachment of enterotoxigenic Escherichia coli to human intestinal cells
Deneke, C. F., McGowan, K., Thorne, G. M., & Gorbach, S. L. (1983) · Infection and Immunity · doi:10.1128/iai.39.3.1102-1106.1983 · Cited by 45
Authentication of African green monkey cell lines using human short tandem repeat markers
Almeida, J. L., Hill, C. R., & Cole, K. D. (2011) · BMC Biotechnology · doi:10.1186/1472-6750-11-102 · Cited by 43
Establishment and biological characteristics of Luxi cattle fibroblast bank
Liu, C., Guo, Y., Guan, W., Ma, Y., Zhang, H., & Tang, X. (2008) · Tissue and Cell · doi:10.1016/j.tice.2008.04.005 · Cited by 43
False cell lines: The problem and a solution
Masters, J. R. (2002) · Cytotechnology · doi:10.1023/a:1022908930937 · Cited by 44
Cell culture forensics
O’Brien, S. J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.141237598 · Cited by 43
Cross-contamination of human esophageal squamous carcinoma cell lines detected by DNA fingerprint analysis.
van Helden, P. D., Wiid, I. J. F., Albrecht, C., Theron, E., Thornley, A. L., & Hoal, E. G. (1988) · PubMed · Cited by 43
False and mycoplasma-contaminated leukemia-lymphoma cell lines: time for a reappraisal
Drexler, H. G., Dirks, W. G., MacLeod, R. A. F., & Uphoff, C. C. (2017) · International Journal of Cancer · doi:10.1002/ijc.30530 · Cited by 45
Enterococcus faecalis : an overlooked cell invader
Archambaud, C., Nunez, N., da Silva, R. A. G., Kline, K. A., & Serror, P. (2024) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00069-24 · Cited by 44
Characterization of interferons induced by bacteria and interferon-producing leukocytes in human peripheral blood
Rönnblom, L., Forsgren, A., & ALM, G. V. (1983) · Infection and Immunity · doi:10.1128/iai.40.1.126-132.1983 · Cited by 41
HeLa Nucleic Acid Contamination in The Cancer Genome Atlas Leads to the Misidentification of Human Papillomavirus 18
Cantalupo, P. G., Katz, J. P., & Pipas, J. M. (2015) · Journal of Virology · doi:10.1128/jvi.03365-14 · Cited by 40
Characterization of Genetic Markers for In Vitro Cell Line Identification of the Marine Sponge Axinella corrugata
Lopez, J. V. (2002) · Journal of Heredity · doi:10.1093/jhered/93.1.27 · Cited by 40
Review: Veterinary Sources of Nonrodent Monoclonal Antibodies: Interspecific and Intraspecific Hybridomas
Groves, D. J., & Morris, B. A. (2000) · Hybridoma · doi:10.1089/02724570050109602 · Cited by 40
The UK Stem Cell Bank: Its role as a public research resource centre providing access to well-characterised seed stocks of human stem cell lines
Healy, L. E., HUNT, C., YOUNG, L., & Stacey, G. (2005) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2005.07.019 · Cited by 40
ED27 trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype
Kniss, D. A., Xie, Y., Li, Y., Kumar, S., Linton, E. A., Cohen, P., Fan-Havard, P., Redman, C. W. G., & Sargent, I. L. (2002) · Placenta · doi:10.1053/plac.2001.0749 · Cited by 39
Alkaline phosphatase isozymes in cultured human cancer cells
Herz, F. (1985) · Cellular and Molecular Life Sciences · doi:10.1007/bf01949989 · Cited by 38
Lethal, Potentially Lethal, and Nonlethal Damage Induction by Heavy Ions in Cultured Human Cells
Todd, P. W., Wood, J. C. S., Walker, J. T., & Weiss, S. J. (1985) · Radiation Research · doi:10.2307/3576625 · Cited by 37
Essential role for gene profiling analysis in the authentication of human cell lines
YOSHINO, K., IIMURA, E., SAIJO, K., IWASE, S., FUKAMI, K., OHNO, T., OBATA, Y., & NAKAMURA, Y. (2006) · Human Cell · doi:10.1111/j.1749-0774.2005.00007.x · Cited by 44
Toward a new autoantibody diagnostic orthodoxy: understanding the bad, good and indifferent
Fritzler, M. J. (2012) · Autoimmunity Highlights · doi:10.1007/s13317-012-0030-7 · Cited by 35
Responsibility for truth in research
Nelson‐Rees, W. A. (2001) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2001.0873 · Cited by 35
Self-citation
Development of insect cell lines: Virus susceptibility and applicability to prawn cell culture
Lynn, D. E. (1999) · Methods in Cell Science · doi:10.1023/a:1009824207045 · Cited by 35
Isoenzyme analysis as a rapid method for the examination of the species identity of cell cultures
Steube, K. G., Grunicke, D., & Drexler, H. G. (1995) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/bf02633971 · Cited by 35
Cell line provenance
Freshney, R. I. (2002) · Cytotechnology · doi:10.1023/a:1022949730029 · Cited by 34
Characterization and authentication of cancer cell lines: an overview
Reid, Y. A. (2011) · Methods in Molecular Biology · doi:10.1007/978-1-61779-080-5_4 · Cited by 34
Discovery of HeLa Cell Contamination in HES Cells: Call for Cell Line Authentication in Reproductive Biology Research
Kniss, D. A., & Summerfield, T. L. (2014) · Reproductive Sciences · doi:10.1177/1933719114522518 · Cited by 33
A bioinformatics analysis of the cell line nomenclature
Sarntivijai, S., Ade, A. S., Athey, B. D., & States, D. J. (2008) · Bioinformatics · doi:10.1093/bioinformatics/btn502 · Cited by 32
Cell Characterization by Use of Multiple Genetic Markers
Hukku, B., Halton, D. M., Mally, M. B., & Peterson, W. D. (1984) · Advances in experimental medicine and biology · doi:10.1007/978-1-4615-9376-8_2 · Cited by 32
A comprehensive review of Hep-2 cell line in translational research for laryngeal cancer.
Gorphe, P. (2019) · PubMed · Cited by 30
Generating and working with Drosophila cell cultures: Current challenges and opportunities
Luhur, A., Klueg, K. M., & Zelhof, A. C. (2018) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.339 · Cited by 31
Human Tumor Lines for Cancer Research
Fogh, J. (1986) · Cancer Investigation · doi:10.3109/07357908609038260 · Cited by 31
Animal Systems for Translational Research in Radiation Oncology
Taghian, A. G. (1999) · Acta Oncologica · doi:10.1080/028418699432518 · Cited by 30

Authors: Nelson-Rees, W. A., Daniels, D. W., & Flandermeyer, R. R.
Affiliations: Cell Culture Department, University of California, Berkeley, Naval Biosciences Laboratory, Oakland, California 94625
University of California, Berkeley (US)
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1981-04-24
Volume: 212
Issue: 4493
Pages: 446-452
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.6451928
OpenAlex: W1964255717
Cited by: 329
References: 103
Citations by year: 2026: 8 · 2025: 3 · 2024: 5 · 2023: 6 · 2022: 7 · 2021: 18 · 2020: 7 · 2019: 9 · 2018: 7 · 2017: 8 · 2016: 8 · 2015: 6 · 2014: 11 · 2013: 10 · 2012: 16
FWCI: 0.85
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
Keywords: HeLa cells, human cell lines, cell line contamination, contamination detection
MeSH terms: Animals, Cell Line, Cells, Cultured, Glucosephosphate Dehydrogenase, Humans, Isoenzymes, Karyotyping, Phosphoglucomutase, Species Specificity

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