Strain-Dependent Epithelial Defects in Mice Lacking the EGF Receptor
Abstract
Mice and cells lacking the epidermal growth factor receptor (EGFR) were generated to examine its physiological role in vivo. Mutant fetuses are retarded in growth and die at mid-gestation in a 129/Sv genetic background, whereas in a 129/Sv × C57BL/6 cross some survive until birth and even to postnatal day 20 in a 129/Sv × C57BL/6 × MF1 background. Death in utero probably results from a defect in the spongiotrophoblast layer of the placenta. Newborn mutant mice have open eyes, rudimentary whiskers, immature lungs, and defects in the epidermis, correlating with the expression pattern of the EGFR as monitored by β-galactosidase activity. These defects are probably cell-autonomous because chimeric mice generated with EGFR -/- embryonic stem cells contribute small amounts of mutant cells to some organs. These results indicate that the EGFR regulates epithelial proliferation and differentiation and that the genetic background influences the resulting phenotype.
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In control tissue sections, EGFR localizes not only in the basal layer but also in suprabasal layers.
“Staining of control tissue sections showed localization of EGFR not only in the basal layer, as expected30, but also in suprabasal layers (Supplementary Fig. 7a, b).”
From E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning (2017) · cites this paper as [30]
Loss of epidermal EGFR is associated with skin barrier dysfunction.
“As loss of epidermal EGFR is associated with skin barrier dysfunction30”
From E-cadherin integrates mechanotransduction and EGFR signaling to control junctional tissue polarization and tight junction positioning (2017) · cites this paper as [30]
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- Authors
- Sibilia, M., & Wagner, E. F.
- Affiliations
- Research Institute of Molecular Pathology (AT)
- Venue
- Science
- Published
- 1995-07-14
- Volume
- 269
- Issue
- 5221
- Pages
- 234-238
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2002273560
- Cited by
- 1,002
- References
- 0
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 13 · 2023: 8 · 2022: 14 · 2021: 17 · 2020: 19 · 2019: 20 · 2018: 25 · 2017: 18 · 2016: 19 · 2015: 30 · 2014: 28 · 2013: 33 · 2012: 31
- FWCI
- 31.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- epidermal growth factor receptor, epithelial defect, epithelial cell proliferation, epithelial differentiation, placenta, spongiotrophoblast, genetic background, chimeric mice, embryonic stem cells, fetal growth restriction, beta-galactosidase expression
- MeSH terms
- Animals, Cell Differentiation, Cell Division, Epithelial Cells, Female, Embryonic and Fetal Development, Hematopoiesis, Lung, Male, Mice, Inbred Strains, Mutation, Phenotype, Placenta, Skin, Species Specificity, Stem Cells, Trophoblasts, Gene Targeting, Mice, ErbB Receptors
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