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The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

RNA editing with CRISPR-Cas13

Cox, David B. T., Gootenberg, Jonathan S., Abudayyeh, Omar O., Franklin, Brian, Kellner, Max J., Joung, Julia, Zhang, Feng·2017·Science
DOI 10.1126/science.aaq0180PMC PMC5793859
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Abstract

Precise transcriptome engineering: Efficient and precise RNA editing to correct disease-relevant transcripts holds great promise for treating genetic disease. Cox et al. took advantage of the ability of Cas13b, an effector from a type VI CRISPR-Cas system, to target specific RNAs directly (see the Perspective by Yang and Chen). They fused Cas13b with the ADAR2 adenosine deaminase domain and used rational protein engineering to improve the resultant enzyme. These approaches yielded an RNA knockdown and editing platform that allowed efficient and specific RNA depletion and correction in mammalian cells. Science , this issue p. 1019 ; see also p. 996

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  • CRISPR/Cas13a systems can cleave RNA molecules and emerge as an answer to RNA viruses, although ongoing research focuses on identifying most potent and specific Cas13 variants.

    “CRISPR/Cas13a systems also emerge as an answer to RNA viruses do their capacity to cleave RNA molecules, although due to its novelty ongoing research still focuses on identifying the most potent and specific Cas13 variants [173,174].”

    From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [173]

    The Introduction (page unavailable) says Cas13 enzymes cleave RNA and describes testing Cas13 variants to identify one with efficient, specific RNA knockdown. The Comprehensive Characterization section (page unavailable) includes Cas13a among the variants tested. Our copy does not present Cas13a as an answer to RNA viruses; its reported applications concern RNA knockdown and editing in mammalian cells. (LLM, conf=0.88)

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Research works printed but not linked: 32

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Metadata

Authors
Cox, D. B. T., Gootenberg, J. S., Abudayyeh, O. O., Franklin, B., Kellner, M. J., Joung, J., & Zhang, F.
Affiliations
Broad Institute of Massachusetts Institute of Technology (MIT) and Harvard, Cambridge, MA 02142, USA.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2017-11-24
Volume
358
Issue
6366
Pages
1019-1027
ISSN
  • 0036-8075
  • 1095-9203
DOI
10.1126/science.aaq0180
PMC
PMC5793859
Cited by
1,834
References
6
Language
en
Funders
  • National Institutes of Health (award352307, 1R01-HG009761, award352308, 1R01-MH110049, award352309, 1DP1-HL141201, award352311, T32GM007753, award352317, 1F30-CA210382)
  • Paul and Daisy Soros Fellowships for New Americans (award352310)
  • Howard Hughes Medical Institute (award352313)
  • Simons Foundation (award352312)
  • Paul G. Allen Family Foundation (award352315)
  • New York Stem Cell Foundation (award352314)
  • Vallee Foundation (award352316)

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