Effect of Growth Rate on Resistance of Candida albicans Biofilms to Antifungal Agents

Baillie, George S.; Douglas, L. Julia

doi:10.1128/aac.42.8.1900

Cited by 234Antifungal resistance and susceptibilityBacterial biofilms and quorum sensingFungal and yeast genetics research

Effect of Growth Rate on Resistance of Candida albicans Biofilms to Antifungal Agents

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Baillie, George S., Douglas, L. Julia·1998·Antimicrobial Agents and Chemotherapy

DOI 10.1128/aac.42.8.1900PMC PMC105707

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Abstract

A perfused biofilm fermentor, which allows growth-rate control of adherent microbial populations, was used to assess whether the susceptibility of Candida albicans biofilms to antifungal agents is dependent on growth rate. Biofilms were generated under conditions of glucose limitation and were perfused with drugs at a high concentration (20 times the MIC). Amphotericin B produced a greater reduction in the number of daughter cells in biofilm eluates than ketoconazole, fluconazole, or flucytosine. Similar decreases in daughter cell counts were observed when biofilms growing at three different rates were perfused with amphotericin B. In a separate series of experiments, intact biofilms, resuspended biofilm cells, and newly formed daughter cells were removed from the fermentor and were exposed to a lower concentration of amphotericin B for 1 h. The susceptibility profiles over a range of growth rates were then compared with those obtained for planktonic cells grown at the same rates under glucose limitation in a chemostat. Intact biofilms were resistant to amphotericin B at all growth rates tested, whereas planktonic cells were resistant only at low growth rates (</=0.13 h-1). Cells resuspended from biofilms were less resistant than intact biofilm populations but more resistant than daughter cells; the susceptibilities of both these cell types were largely independent of growth rate. Our findings indicate that the amphotericin B resistance of C. albicans biofilms is not simply due to a low growth rate but depends on some other feature of the biofilm mode of growth.

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Growth rate plays only a minor role in Candida species biofilm drug resistance.
“Baillie and Douglas [160] have shown that, at lower growth rates, planktonic cells are more resistant to amphotericin B, and biofilms are equally resistant over a range of growth rates, thereby suggesting that growth rate plays only a minor role in Candida species biofilm drug resistance.”
From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [160]

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Authors: Baillie, G. S., & Douglas, L. J.
Affiliations: University of Glasgow (GB)
ORCID: Baillie, George S.
Douglas, L. Julia
Venue: Antimicrobial Agents and Chemotherapy
Publisher: American Society for Microbiology
Published: 1998-08
Volume: 42
Issue: 8
Pages: 1900-1905
ISSN: 0066-4804 (ISSN-L)
1098-6596
DOI: 10.1128/aac.42.8.1900
PMC: PMC105707
OpenAlex: W1921586209
Cited by: 234
References: 26
Citations by year: 2025: 6 · 2024: 4 · 2023: 1 · 2022: 6 · 2021: 6 · 2020: 9 · 2019: 2 · 2018: 4 · 2017: 14 · 2016: 9 · 2015: 16 · 2014: 16 · 2013: 15 · 2012: 10
FWCI: 3.78
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Antimicrobial Agents and Chemotherapy (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Sir Jules Thorn Charitable Trust
Topics: Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords: Candida albicans, biofilm, antifungal resistance, growth rate, amphotericin B, ketoconazole, fluconazole, 5-fluorocytosine, glucose limitation, planktonic cells, daughter cells
MeSH terms: Amphotericin B, Antifungal Agents, Candida albicans, Drug Resistance, Microbial, Biofilms

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Effect of Growth Rate on Resistance of Candida albicans Biofilms to Antifungal Agents

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