Cited by 204SARS-CoV-2 and COVID-19 ResearchImmune responses and vaccinationsvaccines and immunoinformatics approaches

S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit

Open access
Nguyen-Contant, Phuong, Embong, A. Karim, Kanagaiah, Preshetha, Chaves, Francisco A., Yang, Hongmei, Branche, Angela R., Topham, David J., Sangster, Mark Y.·2020·mBio

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Abstract

The recent rapid worldwide spread of SARS-CoV-2 has established a pandemic of potentially serious disease in the highly susceptible human population. Key issues are whether humans have preexisting immune memory that provides some protection against SARS-CoV-2 and whether SARS-CoV-2 infection generates lasting immune protection against reinfection. Our analysis focused on pre- and postinfection IgG and IgG memory B cells (MBCs) reactive to SARS-CoV-2 proteins. Most importantly, we demonstrate that infection generates both IgG and IgG MBCs against the novel receptor binding domain and the conserved S2 subunit of the SARS-CoV-2 spike protein. Thus, even if antibody levels wane, long-lived MBCs remain to mediate rapid antibody production. Our study results also suggest that SARS-CoV-2 infection strengthens preexisting broad coronavirus protection through S2-reactive antibody and MBC formation.

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  • The S2 domain of SARS-CoV-2 spike is more conserved with other coronaviruses.

    “the S2 domain of SARS-CoV-2 spike is more conserved with other coronaviruses”
  • SARS-CoV-2 neutralizing antibody responses generally develop from naive B cells, not from pre-existing cross-reactive memory B cells.

    “The data also indicate that SARS-CoV-2 neutralizing antibody responses generally develop from naive B cells, not from pre-existing cross-reactive memory B cells (Anderson et al., 2020; Ng et al., 2020; Nguyen-Contant et al., 2020; Shrock et al., 2020; Song et al., 2020).”

    From Adaptive immunity to SARS-CoV-2 and COVID-19 (2021) · cites this paper as Nguyen-Contant et al., 2020

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Authors
Nguyen-Contant, P., Embong, A. K., Kanagaiah, P., Chaves, F. A., Yang, H., Branche, A. R., Topham, D. J., & Sangster, M. Y.
Affiliations
  • David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USA
  • University of Rochester Medicine (US)
Corresponding author
Sangster, Mark Y.
Venue
mBio
Published
2020-10-27
Volume
11
Issue
5
ISSN
  • 2150-7511 (ISSN-L)
  • 2161-2129
OpenAlex
W3087903445
Cited by
204
References
35
Citations by year
2026: 3 · 2025: 13 · 2024: 16 · 2023: 29 · 2022: 60 · 2021: 75 · 2020: 8
FWCI
5.64
Type
journal article
Language
en
Open access
Locations
  • mBio (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
HHS | NIH | National Institute of Allergy and Infectious Diseases (HHSN272201400005C)
Topics
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords
SARS-CoV-2, COVID-19 pandemic, S protein, S2 subunit, receptor binding domain, memory B cells, IgG, antibody response, pre-existing immunity, reinfection, cross-reactive antibodies, coronavirus
MeSH terms
Betacoronavirus, COVID-19, SARS-CoV-2, Coronavirus Nucleocapsid Proteins, Adult, Antibodies, Viral, B-Lymphocytes, Convalescence, Cross Reactions, Female, Humans, Immunoglobulin G, Immunologic Memory, Male, Middle Aged, Phosphoproteins, Pneumonia, Viral, Coronavirus Infections, Nucleocapsid Proteins, Protein Subunits, Protein Interaction Domains and Motifs, Pandemics, Healthy Volunteers, Spike Glycoprotein, Coronavirus

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