CRISPR-Mediated Adaptive Immune Systems in Bacteria and Archaea

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116

A human gut microbial gene catalogue established by metagenomic sequencing

MetaHIT Consortium, Qin, J., Li, R., Raes, J., Arumugam, M., Burgdorf, K. S., Manichanh, C., Nielsen, T., Pons, N., Levenez, F., et al. (2010) · Nature · doi:10.1038/nature08821 · Cited by 11,624

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947

CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III

Deltcheva, E., Chylinski, K., Sharma, C. M., Gonzales, K., Chao, Y., Pirzada, Z. A., Eckert, M. R., Vogel, J., & Charpentier, E. (2011) · Nature · doi:10.1038/nature09886 · Cited by 2,563

CRISPR/Cas, the Immune System of Bacteria and Archaea

Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

Brouns, S. J. J., Jore, M. M., Lundgren, M., Westra, E. R., Slijkhuis, R. J. H., Snijders, A. P. L., Dickman, M. J., Makarova, K. S., Koonin, E. V., & van der Oost, J. (2008) · Science · doi:10.1126/science.1159689 · Cited by 2,460

Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product

Ishino, Y., Shinagawa, H., Makino, K., Amemura, M., & Nakata, A. (1987) · Journal of Bacteriology · doi:10.1128/jb.169.12.5429-5433.1987 · Cited by 2,421

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

Garneau, J. E., Dupuis, M.-È., Villion, M., Romero, D. A., Barrangou, R., Boyaval, P., Fremaux, C., Horvath, P., Magadán, A. H., & Moineau, S. (2010) · Nature · doi:10.1038/nature09523 · Cited by 2,336

Evolution and classification of the CRISPR–Cas systems

Makarova, K. S., Haft, D. H., Barrangou, R., Brouns, S. J. J., Charpentier, E., Horvath, P., Moineau, S., Mojica, F. J. M., Wolf, Y. I., Yakunin, A. F., et al. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2577 · Cited by 2,331

Viruses in the sea

Suttle, C. A. (2005) · Nature · doi:10.1038/nature04160 · Cited by 2,186

Efficient multiplex mutagenesis by RNA-guided Cas9 and its use in the characterization of regulatory elements in the AGAMOUS gene

Yan, W., Chen, D., & Kaufmann, K. (2016) · Plant Methods · doi:10.1186/s13007-016-0125-7 · Cited by 50

A dynamin‐like protein involved in bacterial cell membrane surveillance under environmental stress

Sawant, P., Eißenberger, K., Karier, L., Mascher, T., & Bramkamp, M. (2015) · Environmental Microbiology · doi:10.1111/1462-2920.13110 · Cited by 47

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

Carabias, A., Fuglsang, A., Temperini, P., Pape, T., Sofos, N., Stella, S., Erlendsson, S., & Montoya, G. (2021) · Nature Communications · doi:10.1038/s41467-021-24707-3 · Cited by 44

CRISPR–Cas-mediated transcriptional control and epi-mutagenesis

Gardiner, J., Ghoshal, B., Wang, M., & Jacobsen, S. E. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac033 · Cited by 46

Thioredoxin targets fundamental processes in a methane-producing archaeon, Methanocaldococcus jannaschii

Susanti, D., Wong, J. H. H., Vensel, W. H., Loganathan, U., DeSantis, R., Schmitz, R. A., Balsera, M., Buchanan, B. B., & Mukhopadhyay, B. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1324240111 · Cited by 46

P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri

Huan, Y. W., Torraca, V., Brown, R., Fa-arun, J., Miles, S. L., Oyarzún, D. A., Mostowy, S., & Wang, B. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.2c00465 · Cited by 45

A CRISPR method for genome engineering

Wilkinson, R. A., & Wiedenheft, B. (2014) · F1000Prime Reports · doi:10.12703/p6-3 · Cited by 46

Structural insights into the inactivation of CRISPR-Cas systems by diverse anti-CRISPR proteins

Zhu, Y., Zhang, F., & Huang, Z. (2018) · BMC Biology · doi:10.1186/s12915-018-0504-9 · Cited by 47

CRISPR: a new principle of genome engineering linked to conceptual shifts in evolutionary biology

Koonin, E. V. (2019) · Biology & Philosophy · doi:10.1007/s10539-018-9658-7 · Cited by 44

Tuning CRISPR-Cas9 Gene Drives in Saccharomyces cerevisiae

Roggenkamp, E., Giersch, R. M., Schrock, M. N., Turnquist, E., Halloran, M., & Finnigan, G. C. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.117.300557 · Cited by 45

Emerging applications of gene editing technologies for the development of climate-resilient crops

Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 55

CRISPR-based herd immunity can limit phage epidemics in bacterial populations

Payne, P., Geyrhofer, L., Barton, N., & Bollback, J. P. (2018) · eLife · doi:10.7554/elife.32035 · Cited by 38

Cmr4 is the slicer in the RNA-targeting Cmr CRISPR complex

Zhu, X. Q., & Ye, K. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1355 · Cited by 43

Quantitative proteomics analysis of an ethanol- and a lactate-producing mutant strain of Synechocystis sp. PCC6803

Borirak, O., de Koning, L. J., van der Woude, A. D., Hoefsloot, H. C. J., Dekker, H. L., Roseboom, W., de Koster, C. G., & Hellingwerf, K. J. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0294-z · Cited by 42

Just how Lamarckian is CRISPR-Cas immunity: the continuum of evolvability mechanisms

Koonin, E. V., & Wolf, Y. I. (2016) · Biology Direct · doi:10.1186/s13062-016-0111-z · Cited by 42

The Versatile Type V CRISPR Effectors and Their Application Prospects

Tong, B., Dong, H., Cui, Y., Jiang, P., Jin, Z., & Zhang, D. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.622103 · Cited by 45

Towards Point of Care CRISPR-Based Diagnostics: From Method to Device

Chen, H., Zhou, X., Wang, M., & Ren, L. (2023) · Journal of Functional Biomaterials · doi:10.3390/jfb14020097 · Cited by 40

Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system

Chang, Y., Su, T., Qi, Q., & Liang, Q. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0594-4 · Cited by 41

AcrHub: an integrative hub for investigating, predicting and mapping anti-CRISPR proteins

Wang, J., Dai, W., Li, J., Li, Q., Xie, R., Zhang, Y., Stubenrauch, C. J., & Lithgow, T. J. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa951 · Cited by 41

The CRISPR-associated Cas4 protein Pcal_0546 from Pyrobaculum calidifontis contains a [2Fe-2S] cluster: crystal structure and nuclease activity

Lemak, S., Nocek, B. P., Beloglazova, N. G., Skarina, T., Flick, R., Brown, G., Joachimiak, A., Savchenko, A. V., & Yakunin, A. F. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku797 · Cited by 43

CRISPR/Cas9 technology and its application in horticultural crops

Liu, Y., Zhang, C., Wang, X., Li, X., & You, C. (2022) · Horticultural Plant Journal · doi:10.1016/j.hpj.2022.04.007 · Cited by 41

Comparative genome analysis and identification of competitive and cooperative interactions in a polymicrobial disease

Endo, A., Watanabe, T., Ogata, N., Nozawa, T., Aikawa, C., Arakawa, S., Maruyama, F., Izumi, Y., & Nakagawa, I. (2014) · The ISME Journal · doi:10.1038/ismej.2014.155 · Cited by 40

Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals: Opportunities and prospects

Mitra, S., Anand, U., Ghorai, M., Kant, N., Kumar, M., Radha, Jha, N. K., Swamy, M. K., Proćków, J., de la Lastra, J. M. P., et al. (2022) · Biotechnology and Bioengineering · doi:10.1002/bit.28260 · Cited by 39

The history and market impact of CRISPR RNA-guided nucleases

van Erp, P. B. G., Bloomer, G., Wilkinson, R. A., & Wiedenheft, B. (2015) · Current Opinion in Virology · doi:10.1016/j.coviro.2015.03.011 · Cited by 40

Real-Time Observation of Target Search by the CRISPR Surveillance Complex Cascade

Xue, C., Zhu, Y., Zhang, X., Shin, Y., & Sashital, D. G. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.11.110 · Cited by 39

Computational Analysis Concerning the Impact of DNA Accessibility on CRISPR-Cas9 Cleavage Efficiency

Chung, C.-H., Allen, A. G., Sullivan, N. T., Atkins, A., Nonnemacher, M. R., Wigdahl, B. L., & Dampier, W. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.10.008 · Cited by 40

Cold adaptation of the Antarctic haloarchaea Halohasta litchfieldiae and Halorubrum lacusprofundi

Williams, T. J., Liao, Y., Ye, J., Kuchel, R. P., Poljak, A., Raftery, M. J., & Cavicchioli, R. (2017) · Environmental Microbiology · doi:10.1111/1462-2920.13705 · Cited by 38

The expanded development and application of CRISPR system for sensitive nucleotide detection

Jia, F., Li, X., Zhang, C., & Tang, X. (2020) · Protein & Cell · doi:10.1007/s13238-020-00708-8 · Cited by 38

Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects

Bhatta, B. P., & Malla, S. (2020) · Plants · doi:10.3390/plants9101360 · Cited by 40

Recent advances on Candida albicans biology and virulence

Sellam, A., & Whiteway, M. S. (2016) · F1000Research · doi:10.12688/f1000research.9617.1 · Cited by 39

CRISPR/Cas9 in rice can induce new mutations in later generations, leading to chimerism and unpredicted segregation of the targeted mutation

Ishizaki, T. (2016) · Molecular Breeding · doi:10.1007/s11032-016-0591-7 · Cited by 37

SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense System

Wang, S., Wan, M., Huang, R., Zhang, Y., Xie, Y., Wei, Y., Ahmad, M., Wu, D., Hong, Y., Deng, Z., et al. (2021) · mBio · doi:10.1128/mbio.00613-21 · Cited by 37

Untangling the origin of viruses and their impact on cellular evolution

Nasir, A., Sun, F., Kim, K. M., & Caetano‐Anollés, G. (2015) · Annals of the New York Academy of Sciences · doi:10.1111/nyas.12735 · Cited by 39

The RNA- and DNA-targeting CRISPR–Cas immune systems of Pyrococcus furiosus

Terns, R. M., & Terns, M. P. (2013) · Biochemical Society Transactions · doi:10.1042/bst20130056 · Cited by 36

A CRISPR CASe for high-throughput silencing

Heintze, J., Luft, C., & Ketteler, R. (2013) · Frontiers in Genetics · doi:10.3389/fgene.2013.00193 · Cited by 38

Accurate computational prediction of the transcribed strand of CRISPR non-coding RNAs

Biswas, A., Fineran, P. C., & Brown, C. M. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu114 · Cited by 36

Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)

Guo, M., Ren, K., Zhu, Y., & Huang, Z. (2018) · Worldwide Protein Data Bank · doi:10.2210/pdb6aeb/pdb · Cited by 37

Unlocking Genome Editing: Advances and Obstacles in CRISPR/Cas Delivery Technologies

Kaupbayeva, B., Tsoy, A., Safarova, Y., Nurmagambetova, A., Murata, H., Matyjaszewski, K. A., & Askarova, S. (2024) · Journal of Functional Biomaterials · doi:10.3390/jfb15110324 · Cited by 40

Application of TALEs, CRISPR/Cas and sRNAs as trans-acting regulators in prokaryotes

Copeland, M. F., Politz, M. C., & Pfleger, B. F. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.02.010 · Cited by 35

A Perspective on the Future of High-Throughput RNAi Screening: Will CRISPR Cut Out the Competition or Can RNAi Help Guide the Way?

Taylor, J., & Woodcock, S. A. (2015) · SLAS DISCOVERY · doi:10.1177/1087057115590069 · Cited by 35

Diverse and unique viruses discovered in the surface water of the East China Sea

Wu, S., Zhou, L., Zhou, Y., Wang, H., Xiao, J., Yan, S., & Wang, Y. (2020) · BMC Genomics · doi:10.1186/s12864-020-06861-y · Cited by 35

Engineering Resistance Against Viruses in Field Crops Using CRISPRCas9

Hinge, V. R., Chavhan, R. L., Kale, S. P., Suprasanna, P., & Kadam, U. S. (2021) · Current Genomics · doi:10.2174/1389202922666210412102214 · Cited by 35

Advances in Editing Silkworms (Bombyx mori) Genome by Using the CRISPR-Cas System

Baci, G.-M., Cucu, A.-A., Giurgiu, A.-I., Muscă, A. S., Bagameri, L., MOISE, A. R., Bobiş, O., Rațiu, A. C., & Dezmirean, D. S. (2021) · Insects · doi:10.3390/insects13010028 · Cited by 37

Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

Sedláček, I., Holochová, P., Busse, H., Koublová, V., Králová, S., Švec, P., Sobotka, R., Staňková, E., Pilný, J., Šedo, O., et al. (2022) · Microorganisms · doi:10.3390/microorganisms10040704 · Cited by 35

Archaea as a Model System for Molecular Biology and Biotechnology

Lise, F. D., Iacono, R., Moracci, M., Strazzulli, A., & Cobucci‐Ponzano, B. (2023) · Biomolecules · doi:10.3390/biom13010114 · Cited by 34

Programmable type III-A CRISPR-Cas DNA targeting modules

Ichikawa, H. T., Cooper, J. C., Lo, L. F., Potter, J., Terns, R. M., & Terns, M. P. (2017) · PLoS ONE · doi:10.1371/journal.pone.0176221 · Cited by 34

Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS

Majsec, K., Bolt, E. L., & Ivančić-Baće, I. (2016) · BMC Microbiology · doi:10.1186/s12866-016-0643-5 · Cited by 34

CRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases

Xia, Y., Rao, R., Xiong, M., He, B., Zheng, B., Jia, Y., Li, Y., & Yang, Y. (2024) · Analytical Chemistry · doi:10.1021/acs.analchem.3c04363 · Cited by 33

Gene regulation by engineered CRISPR-Cas systems

Fineran, P. C., & Dy, R. L. V. (2014) · Current Opinion in Microbiology · doi:10.1016/j.mib.2014.02.007 · Cited by 33

Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications

Pyzocha, N., & Chen, S. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.7b00800 · Cited by 33

Recruitment of Reverse Transcriptase-Cas1 Fusion Proteins by Type VI-A CRISPR-Cas Systems

Toro, N., Mestre, M. R., Martínez‐Abarca, F., & González-Delgado, A. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02160 · Cited by 33

Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domains

Gu, Y., Li, H., Deep, A., Enustun, E., Zhang, D., & Corbett, K. D. (2025) · Molecular Cell · doi:10.1016/j.molcel.2024.12.004 · Cited by 32

The Adaptive Immune System of Haloferax volcanii

Maier, L., Dyall‐Smith, M., & Marchfelder, A. (2015) · Life · doi:10.3390/life5010521 · Cited by 32

DNA on the move: mechanisms, functions and applications of transposable elements

Schmitz, M., & Querques, I. (2023) · FEBS Open Bio · doi:10.1002/2211-5463.13743 · Cited by 41

CRISPR-Cas System: The Powerful Modulator of Accessory Genomes in Prokaryotes

Butiuc-Keul, A. L., Farkas, A., Carpa, R., & Iordache, D. (2021) · Microbial Physiology · doi:10.1159/000516643 · Cited by 31

CRISPR/Cas9-Mediated Genome Editing of Mouse Small Intestinal Organoids

Schwank, G., & Clevers, H. C. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-3603-8_1 · Cited by 32

Characterization of a Novel Composite Staphylococcal Cassette Chromosome mec (SCC mec -SCC cad / ars / cop ) in the Neonatal Sepsis-Associated Staphylococcus capitis Pulsotype NRCS-A

Martins-Simões, P., Rasigade, J., Lemriss, H., Butin, M., Ginévra, C., Lemriss, S., Goering, R. V., Ibrahimi, A., Picaud, J. C., Kabbaj, S. E., et al. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01576-13 · Cited by 31

Nanotechnology‐based CRISPR/Cas9 delivery system for genome editing in cancer treatment

Zhou, S., Li, Y., Wu, Q., & Gong, C. (2024) · MedComm – Biomaterials and Applications · doi:10.1002/mba2.70 · Cited by 30

CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells

Wang, X., Huang, X., Fang, X., Zhang, Y., & Wang, W. (2016) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2016.95 · Cited by 30

Parasitoids as drivers of symbiont diversity in an insect host

Hafer, N., & Vorburger, C. (2020) · Ecology Letters · doi:10.1111/ele.13526 · Cited by 30

Strengthening the CAR‐T cell therapeutic application using CRISPR/Cas9 technology

Nezhad, M. S., Yazdanifar, M., Abdollahpour‐Alitappeh, M., Sattari, A., Seifalian, A. M., & Bagheri, N. (2021) · Biotechnology and Bioengineering · doi:10.1002/bit.27882 · Cited by 30

SnapShot: CRISPR-RNA-Guided Adaptive Immune Systems

Carter, J. J., & Wiedenheft, B. (2015) · Cell · doi:10.1016/j.cell.2015.09.011 · Cited by 30

Role of Type 2 NAD(P)H Dehydrogenase NdbC in Redox Regulation of Carbon Allocation in Synechocystis

Huokko, T., Muth‐Pawlak, D., Battchikova, N., Allahverdiyeva, Y., & Aro, E. (2017) · PLANT PHYSIOLOGY · doi:10.1104/pp.17.00398 · Cited by 30

Biomaterial-Based CRISPR/Cas9 Delivery Systems for Tumor Treatment

Li, M., Chen, F., Yang, Q., Tang, Q., Xiao, Z., Tong, X., Zhang, Y., Lei, L., & Li, S. (2024) · Biomaterials Research · doi:10.34133/bmr.0023 · Cited by 32

CRISPR/Cas9-Based therapeutics as a promising strategy for management of Alzheimer’s disease: progress and prospects

Khan, M. S., Qureshi, N., Khan, R., Son, Y., & Maqbool, T. (2025) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2025.1578138 · Cited by 37

Global transcriptome analysis of Halolamina sp. to decipher the salt tolerance in extremely halophilic archaea

Kurt‐Kızıldoğan, A., Abanoz-Seçgin, B., & Okay, S. (2016) · Gene · doi:10.1016/j.gene.2016.11.042 · Cited by 29

Advance genome editing technologies in the treatment of human diseases: CRISPR therapy (Review)

Alagöz, M., & Kherad, N. (2020) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2020.4609 · Cited by 32

Current and Prospective Applications of CRISPR-Cas12a in Pluricellular Organisms

Khan, S., & Sallard, E. (2022) · Molecular Biotechnology · doi:10.1007/s12033-022-00538-5 · Cited by 31

Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease

Chen, W., Zhang, H., Zhang, Y., Wang, Y., Gan, J., & Ji, Q. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000496 · Cited by 31

Genome editing in rice mediated by miniature size Cas nuclease SpCas12f

Sukegawa, S., Nureki, O., Toki, S., & Saika, H. (2023) · Frontiers in Genome Editing · doi:10.3389/fgeed.2023.1138843 · Cited by 32

Structure Principles of CRISPR-Cas Surveillance and Effector Complexes

Tsui, T. K. M., & Li, H. (2015) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-060414-033939 · Cited by 28

Repeat Size Determination by Two Molecular Rulers in the Type I-E CRISPR Array

Goren, M. G., Doron, S., Globus, R., Amitai, G., Sorek, R., & Qimron, U. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.08.043 · Cited by 28

Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage

Sun, R., Zhao, Y., Wang, W., Liu, J., & Chen, C. (2023) · Chemical Science · doi:10.1039/d2sc05463a · Cited by 28

NilD CRISPR RNA contributes to X enorhabdus nematophila colonization of symbiotic host nematodes

Veesenmeyer, J. L., Andersen, A. W., Lu, X., Hussa, E. A., Murfin, K. E., Chaston, J. M., Dillman, A. R., Wassarman, K. M., Sternberg, P. W., & Goodrich‐Blair, H. (2014) · Molecular Microbiology · doi:10.1111/mmi.12715 · Cited by 28

Molecular and genomic characterization of pathogenic traits of group A Streptococcus pyogenes

Hamada, S., Kawabata, S., & Nakagawa, I. (2015) · Proceedings of the Japan Academy Series B · doi:10.2183/pjab.91.539 · Cited by 26

Structural basis for inhibition of an archaeal CRISPR–Cas type I-D large subunit by an anti-CRISPR protein

Manav, M. C., van Eck, L., Lin, J., Fuglsang, A., Peng, X., & Brodersen, D. E. (2020) · Nature Communications · doi:10.1038/s41467-020-19847-x · Cited by 27

New Insights on Streptococcus dysgalactiae subsp. dysgalactiae Isolates

Alves‐Barroco, C., Caço, J., Roma‐Rodrigues, C., Fernandes, A. R., Bexiga, R., Oliveira, M., Chambel, L., Tenreiro, R. P., Mato, R., & Santos‐Sanches, I. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.686413 · Cited by 28

Programmable DNA cleavage in vitro by Cas9

Karvelis, T., Gasiūnas, G., & Šikšnys, V. (2013) · Biochemical Society Transactions · doi:10.1042/bst20130164 · Cited by 27

Comparative analysis of CRISPR cassettes from the human gut metagenomic contigs

Gogleva, A. A., Gelfand, M. S., & Artamonova, I. I. (2014) · BMC Genomics · doi:10.1186/1471-2164-15-202 · Cited by 27

Structural and functional characterization of Streptococcus pyogenes Cas2 protein under different pH conditions

Ka, D., Kim, D., Baek, G., & Bae, E. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.07.087 · Cited by 26

Potential metabolic strategies of widely distributed holobionts in the oceanic archipelago of St Peter and St Paul (Brazil)

Rua, C. P. J., Gregoracci, G. B., Santos, E. O., Soares, A. C., Francini-Filho, R. B., & Thompson, F. L. (2015) · FEMS Microbiology Ecology · doi:10.1093/femsec/fiv043 · Cited by 27

Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI

Kaymak, E., Farley, B. M., Hay, S. A., Li, C., Ho, S., Hartman, D. J., & Ryder, S. P. (2016) · Developmental Dynamics · doi:10.1002/dvdy.24426 · Cited by 26

Engineering CRISPR immune systems conferring GLRaV-3 resistance in grapevine

Jiao, B., Hao, X., Liu, Z., Liu, M., Wang, J., Liu, L., Liu, N., Song, R., Zhang, J., Fang, Y., et al. (2022) · Horticulture Research · doi:10.1093/hr/uhab023 · Cited by 29

Structural principles of CRISPR-Cas enzymes used in nucleic acid detection

Das, A., Goswami, H. N., Whyms, C., Sridhara, S., & Li, H. (2022) · Journal of Structural Biology · doi:10.1016/j.jsb.2022.107838 · Cited by 26

CRISPR RNA binding and DNA target recognition by purified Cascade complexes from Escherichia coli

Beloglazova, N. G., Kuznedelov, K. D., Flick, R., Datsenko, K. A., Brown, G., Popovic, A., Lemak, S., Semenova, E., Severinov, K., & Yakunin, A. F. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku1285 · Cited by 26

Crystal Structure of Streptococcus pyogenes Cas1 and Its Interaction with Csn2 in the Type II CRISPR-Cas System

Ka, D., Lee, H., Jung, Y.-D., Kim, K., Seok, C., Suh, N., & Bae, E. (2015) · Structure · doi:10.1016/j.str.2015.10.019 · Cited by 25

mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence

Finnigan, G. C., & Thorner, J. W. (2016) · G3 Genes Genomes Genetics · doi:10.1534/g3.116.029801 · Cited by 26

CRISPR-Cas9 and beyond: what’s next in plant genome engineering

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