Communication—Electrochemical Single Nano-Impacts of Electroactive Shewanella Oneidensis Bacteria onto Carbon Ultramicroelectrode
Open accessAbstract
Electrochemical single nano-impacts of electroactive Shewanella oneidensis bacteria at a 7 μ m diameter carbon fibre ultramicroelectrode in an aqueous potassium phosphate buffer (pH = 7.2) solution containing a redox active probe (potassium ferro- or ferricyanide) is reported. We present chronoamperometric measurements recorded at the ultramicroelectrode polarized at the potential of the steady-state current of the redox probe in solution (oxidation for K 4 Fe(CN) 6 or reduction for K 3 Fe(CN) 6 ) in the presence of bacteria. The shape of current transients associated to single bacteria nano-impacts is compared and discussed as a function of the redox probe in solution and of the ultramicroelectrode applied potential.
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Lee et al. demonstrated a linear relationship between collision frequency f_exp and E. coli concentration C_bac, as expected by Equation 1.
“The authors have demonstrated a linear relationship between the collision frequency f exp (number of step-shaped transients) and the E. coli concentration C bac , as expected by Equation 1 below (in conditions where the mass transport is controlled by diffusion) [10,16,32].”
From Trends in single-impact electrochemistry for bacteria analysis (2023) · cites this paper as [16]
The Summary uses a diffusion-based equation to relate the frequency of step-shaped impacts to bacterial concentration. But this paper measures Shewanella oneidensis at one reported concentration; it does not demonstrate an experimental linear relationship across E. coli concentrations. Pages were not provided in our copy. (LLM, conf=0.84)
Experimental — accuracy not yet measuredFor single S. oneidensis impacts at the potential of steady-state current of potassium ferrocyanide (E ≥ +0.3 V vs Ag/AgCl), a typical stair-step current response is detected, corresponding to adsorption of cells onto the UME.
“In this study, a typical stair-step current response is detected for single S. oneidensis impacts at the potential of steady-state current of potassium ferrocyanide (E ≥ +0.3 V vs. Ag/AgCl), corresponding to the adsorption of cells onto the UME [16].”
From Trends in single-impact electrochemistry for bacteria analysis (2023) · cites this paper as [16]
For S. oneidensis collisions at the potential of steady-state current of potassium ferricyanide (E ≤ +0.1 V vs Ag/AgCl), current spikes (blips) are observed, assigned to short bounce events.
“In contrast, current spikes (blips) are observed for S. oneidensis collisions in the chronoamperometry measurement recorded at the potential of steady-state current of potassium ferricyanide (E ≤ +0.1 V vs. Ag/AgCl), assigned to short bounce events [16].”
From Trends in single-impact electrochemistry for bacteria analysis (2023) · cites this paper as [16]
Lebègue et al. investigated electrochemical single impacts of electroactive S. oneidensis Gram-negative bacteria in two different aqueous redox probes (potassium ferrocyanide or potassium ferricyanide) by applying the appropriate redox potential at a 7 μm carbon UME.
“Following a similar strategy, Lebègue et al. have investigated the electrochemical single impacts of electroactive Shewanella oneidensis (S. oneidensis) Gram-negative bacteria in two different aqueous redox probes (either potassium ferrocyanide or potassium ferricyanide) by applying the appropriate redox potential at a 7 μm carbon UME [16].”
From Trends in single-impact electrochemistry for bacteria analysis (2023) · cites this paper as [16]
These observations point to attraction/repulsion of negatively charged S. oneidensis bacteria by the polarized carbon UME surface as a function of the applied potential.
“These observations point to the attraction/repulsion of negatively charged S. oneidensis bacteria by the polarized carbon UME surface as a function of the applied potential [16].”
From Trends in single-impact electrochemistry for bacteria analysis (2023) · cites this paper as [16]
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- Authors
- Lebègue, E., Costa, N. L., Louro, R. O., & Barrière, F.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Instituto de Tecnologia Química e Biológica António Xavier (PT)
- Venue
- Journal of The Electrochemical Society
- Publisher
- The Electrochemical Society
- Published
- 2020-01-06
- Volume
- 167
- Issue
- 10
- Pages
- 105501
- ISSN
- 0013-4651 (ISSN-L)
- 1945-7111
- OpenAlex
- W3036145237
- Cited by
- 17
- References
- 40
- Citations by year
- 2024: 4 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 2
- FWCI
- 1.17
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of The Electrochemical Society (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- France-Portugal PHC PESSOA program (project 40814ZE)
- European Union’s Horizon 2020 research and innovation program (grant agreement No. 745689)
- Fundação para a Ciência e a Tecnologia Portugal (project LISBOA-01-0145-FEDER-007660)
- Topics
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Keywords
- Shewanella oneidensis, electroactive bacteria, chronoamperometry, potassium ferrocyanide, potassium ferricyanide, redox probe, current transients, bioelectrocatalysis
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