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IL-7 is expressed and secreted by human skeletal muscle cells
Abstract
In addition to generating movement, skeletal muscle may have a function as a secretory organ. The aim of the present study was to identify novel proteins with signaling capabilities secreted from skeletal muscle cells. IL-7 was detected in media conditioned by primary cultures of human myotubes differentiated from satellite cells, and concentrations increased with incubation time. By immunoblotting and real-time RT-PCR IL-7 expression was confirmed at both protein and mRNA levels. Furthermore, with immunofluorescence and specific antisera, multinucleated myotubes were found to coexpress IL-7 and myosin heavy chain. During differentiation of human myotubes from satellite cells, IL-7 expression increased at mRNA and protein levels. In contrast, mRNA expression of the IL-7 receptor was 80% lower in myotubes compared with satellite cells. Incubations with recombinant IL-7 under differentiation conditions caused approximately 35% reduction in mRNA for the terminal myogenic markers myosin heavy chain 2 (MYH2) and myogenin (MYOG), suggesting that IL-7 may act on satellite cells to inhibit development of the muscle fiber phenotype. Alternative routes of cell development were investigated, and IL-7 increased migration of satellite cells by 40% after 48 h in a Transwell system, whereas cell proliferation remained unchanged. In vivo, real-time RT-PCR analysis of musculus vastus lateralis (n = 10) and musculus trapezius (n = 7) biopsies taken from male individuals undergoing a strength training program demonstrated that after 11 wk mean IL-7 mRNA increased by threefold (P = 0.01) and fourfold (P = 0.04), respectively. In conclusion, we have demonstrated that IL-7 is a novel myokine regulated both in vitro and in vivo, and it may play a role in the regulation of muscle cell development.
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“A number of other myokines, including IL‑15 (53) and IL-7 (54) have further been demonstrated to possess anabolic features in rodent models.”
From Muscle–Organ Crosstalk: The Emerging Roles of Myokines (2020) · cites this paper as [54]
In the citing paper
“A post-exercise surge in thymopoiesis is possible considering that skeletal muscle secretes IL-7 (Haugen et al. 2010) which might in turn increase thymic mass and lead to the emergence of recent thymic emigrants (Fry and Mackall 2005).”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Haugen et al. 2010
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Background
Metadata
- Authors
- Haugen, F., Norheim, F., Lian, H., Wensaas, A. J., Dueland, S., Berg, O., Funderud, A., Skålhegg, B. S., Raastad, T., & Drevon, C. A.
- Affiliations
- University of Oslo (NO)
- Norwegian School of Sport Sciences (NO)
- Corresponding author
- Haugen, Fred
- Venue
- American Journal of Physiology-Cell Physiology
- Publisher
- American Physiological Society
- Published
- 2010-04
- Volume
- 298
- Issue
- 4
- Pages
- C807-C816
- ISSN
- 0363-6143 (ISSN-L)
- 1522-1563
- OpenAlex
- W2112164340
- Cited by
- 237
- References
- 50
- Citations by year
- 2026: 14 · 2025: 17 · 2024: 24 · 2023: 14 · 2022: 14 · 2021: 17 · 2020: 20 · 2019: 11 · 2018: 9 · 2017: 7 · 2016: 18 · 2015: 18 · 2014: 8 · 2013: 15 · 2012: 18
- FWCI
- 5.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Physiology-Cell Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
- Keywords
- IL-7, myokines, skeletal muscle, satellite cells, myotubes, myogenesis, IL-7 receptor, myosin heavy chain, myogenin, resistance training, vastus lateralis, trapezius, muscle fiber phenotype
- MeSH terms
- Adult, Cell Differentiation, Cells, Cultured, Culture Media, Humans, Male, Middle Aged, RNA, Messenger, Biomarkers, Interleukin-7, Muscle Fibers, Skeletal, Myosin Heavy Chains, Receptors, Interleukin-7, Muscle Development, Satellite Cells, Skeletal Muscle, Cell Proliferation, Resistance Training
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