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Cited by 145Muscle metabolism and nutritionCardiovascular and exercise physiologyDiet and metabolism studies

Low-fat diet alters intramuscular substrates and reduces lipolysis and fat oxidation during exercise

Coyle, Edward F., Jeukendrup, Asker E., Oseto, Matthew C., Hodgkinson, Bradley J., Zderic, Theodore W.·2001·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.2001.280.3.e391
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Abstract

We determined whether a low-fat diet reduces intramuscular triglyceride (IMTG) concentration, whole body lipolyis, total fat oxidation, and calculated nonplasma fatty acid (FA) oxidation during exercise. Seven endurance-trained cyclists were studied over a 3-wk period during which time they exercised 2 h/day at 70% of maximum O2 uptake VO(2 max) and consumed approximately 4,400 kcal/day. During the 1st wk, their fat intake provided 32% of energy. During the 2nd and 3rd wk, they were randomly assigned to eat 2 or 22% of energy from fat (2%FAT or 22%FAT). Compared with 22%FAT, 2%FAT lowered IMTG concentration and raised muscle glycogen concentration at rest (P < 0.05). Metabolism was studied during 1 h of exercise at 67% VO(2 max) performed in the fasted state. 2%FAT resulted in a 27% reduction (P < 0.05) in total fat oxidation vs. 22%FAT without altering the stable isotopically determined rates of plasma free fatty acid or glucose disappearance. Therefore, 2%FAT reduced calculated nonplasma FA oxidation by 40% in association with a 19% reduction in whole body lipolysis while increasing calculated minimal muscle glycogen oxidation compared with 22%FAT (all P < 0.05). In summary, an extremely low fat (2% of energy) and high-carbohydrate diet lowers whole body lipolysis, total fat oxidation, and nonplasma FA oxidation during exercise in the fasted state in association with a reduced concentration of intramuscular triglyceride.

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Crossref · 10.1152/ajpendo.2001.280.3.e391

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  • A diet with just 22% of energy coming from fat did not reduce intramuscular triglyceride stores.

    “Nevertheless, Coyle et al. [130] demonstrated that a diet with just 22% of energy coming from fat did not reduce IMTG stores.”

    From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [130]

  • It was not until dietary fat intake was limited to 2% of total energy intake that significant reductions in intramuscular triglyceride stores occurred.

    “In fact, it was not until dietary fat intake was limited to 2% of total energy intake that significant reductions in IMTG stores occurred [130].”

    From Nutritional Considerations for the Vegan Athlete (2023) · cites this paper as [130]

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Authors
Coyle, E. F., Jeukendrup, A. E., Oseto, M. C., Hodgkinson, B. J., & Zderic, T. W.
Affiliations
The University of Texas at Austin (US)
ORCID
Coyle, Edward F.
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2001-03-01
Volume
280
Issue
3
Pages
E391-E398
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.2001.280.3.e391
OpenAlex
W2125523156
Cited by
145
References
59
Citations by year
2026: 1 · 2025: 6 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 7 · 2012: 6
FWCI
4.64
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Physiology › Diet and metabolism studies
Keywords
low-fat diet, intramuscular triglycerides, fat oxidation, muscle glycogen, endurance cyclists, fasted exercise, substrate metabolism, high-carbohydrate diet
MeSH terms
Adult, Bicycling, Blood Glucose, Body Composition, Body Weight, Energy Intake, Dietary Carbohydrates, Fasting, Fatty Acids, Fatty Acids, Nonesterified, Glycogen, Humans, Kinetics, Lipolysis, Male, Oxidation-Reduction, Oxygen Consumption, Physical Endurance, Triglycerides, Exercise, Muscle, Skeletal, Diet, Fat-Restricted, Lipid Metabolism

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