MicroRNA-1 and microRNA-133a expression are decreased during skeletal muscle hypertrophy
Abstract
MicroRNAs (miRNAs) are a class of highly conserved, noncoding RNAs involved in posttranscriptional gene regulation. A small number of muscle-specific miRNAs have been identified and shown to have a role in myoblast proliferation and differentiation as well as embryonic muscle growth. The primary objective of the present study was to determine the expression level of the muscle-specific miRNAs in the soleus and plantaris muscles and whether their expression in the plantaris was altered in response to functional overload. Of the miRNAs examined, only miRNA-206 was differentially expressed between soleus and plantaris muscles, as reflected by the sevenfold higher expression in the soleus for both the primary miRNA (pri-miRNA) and mature miRNA (miR). Following 7 days of functional overload, transcript levels for both pri-miRNA-1-2 and pri-miRNA-133a-2 increased by approximately 2-fold, whereas pri-miRNA-206 levels were elevated 18.3-fold. In contrast, expression of miR-1 and miR-133a were downregulated by approximately 50% following overload. The discrepancy between pri-miRNA and miR expression following overload was not explained by a change in the expression of components of the miRNA biogenesis pathway, since Drosha and Exportin-5 transcript levels were significantly increased by 50% in response to functional overload, whereas Dicer expression remained unchanged. These results are the first to report alterations in expression of muscle-specific miRNAs in adult skeletal muscle and suggest miRNAs may have a role in the adaptation to functional overload.
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Muscle-enriched miRNAs such as miR-1 and miR-133a have been implicated in control of growth-related pathways and may modulate translation of genes involved in ribosomal assembly or myogenic adaptation.
“Muscle-enriched miRNAs such as miR-1 and miR-133a have been implicated in the control of growth-related pathways and may modulate the translation of genes involved in ribosomal assembly or myogenic adaptation [198,199].”
From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [198]
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- Authors
- McCarthy, J. J., & Esser, K. A.
- Affiliations
- University of Kentucky (US)
- Venue
- Journal of Applied Physiology
- Publisher
- American Physiological Society
- Published
- 2007-01
- Volume
- 102
- Issue
- 1
- Pages
- 306-313
- ISSN
- 0161-7567 (ISSN-L)
- 8750-7587
- 1522-1601
- OpenAlex
- W2044004072
- Cited by
- 423
- References
- 46
- Citations by year
- 2026: 9 · 2025: 15 · 2024: 12 · 2023: 9 · 2022: 17 · 2021: 19 · 2020: 26 · 2019: 24 · 2018: 21 · 2017: 33 · 2016: 31 · 2015: 32 · 2014: 36 · 2013: 20 · 2012: 26
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR 45617, R01AR045617)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Physiology › Spaceflight effects on biology
- Keywords
- miR-1, microRNA-133a, miR-206, skeletal muscle hypertrophy, functional overload, soleus muscle, plantaris muscle, muscle-specific microRNAs, microRNA biogenesis, Drosha, XPO5, Dicer, post-transcriptional regulation, muscle adaptation
- MeSH terms
- Animals, Gene Expression Regulation, Hypertrophy, Male, Mice, Inbred C57BL, Phenotype, Physical Conditioning, Animal, Muscle, Skeletal, Karyopherins, MicroRNAs, Ribonuclease III, Mice
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