Plasma MicroRNAs as Novel Biomarkers for Endometriosis and Endometriosis-Associated Ovarian Cancer
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Abstract
Purpose: Endometriosis, a largely benign, chronic inflammatory disease, is an independent risk factor for endometrioid and clear cell epithelial ovarian tumors. We aimed to identify plasma miRNAs that can be used to differentiate patients with endometriosis and ovarian cancer from healthy individuals. Experimental Design: We conducted a two-stage exploratory study to investigate the use of plasma miRNA profiling to differentiate between patients with endometriosis, patients with endometriosis-associated ovarian cancer (EAOC), and healthy individuals. In the first stage, using global profiling of more than 1,000 miRNAs via reverse transcriptase quantitative PCR (RT-qPCR) in a 20-patient initial screening cohort, we identified 23 candidate miRNAs, which are differentially expressed between healthy controls (n = 6), patients with endometriosis (n = 7), and patients with EAOC (n = 7) based on the fold changes. In the second stage, the 23 miRNAs were further tested in an expanded cohort (n = 88) of healthy individuals (n = 20), endometriosis (n = 33), EAOC (n = 14), and serous ovarian cancer cases (SOC; n = 21, included as controls). Results: We identified three distinct miRNA signatures with reliable differential expression between healthy individuals, patients with endometriosis, and patients with EAOC. When profiled against the control SOC category, our results revealed different miRNAs, suggesting that the identified signatures are reflective of disease-specific pathogenic mechanisms. This was further supported by the fact that the majority of miRNAs differentially expressed in human EAOCs were mirrored in a double transgenic mouse EAOC model. Conclusion: Our study reports for the first time that distinct plasma miRNA expression patterns may serve as highly specific and sensitive diagnostic biomarkers to discriminate between healthy, endometriosis, and EAOC cases. Clin Cancer Res; 19(5); 1213–24. ©2013 AACR.
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Reduced plasma levels of miR-17-5p, miR-20a, and miR-22 and elevated plasma levels of miR-16, miR-191, and miR-195 have been found in women with endometriosis compared with women without endometriosis.
“Reduced plasma levels of miR-17-5p, miR-20a, and miR-22 [86] and elevated plasma levels of miR-16, miR-191, and miR-195 [87] have been found in women with endometriosis compared with women without endometriosis.”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [87]
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- Authors
- Suryawanshi, S., Vlad, A. M., Lin, H.-M., Mantia-Smaldone, G., Laskey, R., Lee, M., Lin, Y., Donnellan, N., Klein-Patel, M., Lee, T., Mansuria, S., Elishaev, E., Budiu, R., Edwards, R. P., & Huang, X.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Huang, Xin
- ORCID
- Venue
- Clinical Cancer Research
- Published
- 2013-03-01
- Volume
- 19
- Issue
- 5
- Pages
- 1213-1224
- ISSN
- 1078-0432 (ISSN-L)
- 1557-3265
- PMC
- PMC3596045
- OpenAlex
- W2153569033
- Cited by
- 197
- References
- 52
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 10 · 2023: 8 · 2022: 8 · 2021: 18 · 2020: 18 · 2019: 13 · 2018: 18 · 2017: 17 · 2016: 20 · 2015: 30 · 2014: 16 · 2013: 7
- FWCI
- 29.38
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Clinical Cancer Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Magee-Womens Research Institute
- National Institutes of Health (UL1TR000005)
- National Cancer Institute (NIH/NCI 1 R01 CA163462-01, R01CA163462, P30CA047904)
- National Center for Advancing Translational Sciences
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Reproductive Medicine › Ovarian cancer diagnosis and treatment
- Keywords
- endometriosis, endometriosis-associated ovarian cancer, plasma microRNA, diagnostic biomarkers, microRNA signature, serous ovarian cancer, double transgenic mouse model, differentially expressed miRNAs, disease discrimination
- MeSH terms
- Adult, Aged, Animals, Endometriosis, Endometrium, Female, Follow-Up Studies, Humans, Middle Aged, Neoplasm Staging, Ovarian Neoplasms, Prognosis, Retrospective Studies, Biomarkers, Tumor, Gene Expression Regulation, Neoplastic, Survival Rate, Case-Control Studies, Proto-Oncogene Proteins p21(ras), Adenocarcinoma, Clear Cell, Cystadenocarcinoma, Serous, Mice, Knockout, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, PTEN Phosphohydrolase, Mice
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