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mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish

Open access
Ellett, Felix, Pase, Luke, Hayman, John W., Andrianopoulos, Alex, Lieschke, Graham J.·2011·Blood
DOI 10.1182/blood-2010-10-314120
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Abstract

Macrophages and neutrophils play important roles during the innate immune response, phagocytosing invading microbes and delivering antimicrobial compounds to the site of injury. Functional analyses of the cellular innate immune response in zebrafish infection/inflammation models have been aided by transgenic lines with fluorophore-marked neutrophils. However, it has not been possible to study macrophage behaviors and neutrophil/macrophage interactions in vivo directly because there has been no macrophage-only reporter line. To remove this roadblock, a macrophage-specific marker was identified (mpeg1) and its promoter used in mpeg1-driven transgenes. mpeg1-driven transgenes are expressed in macrophage-lineage cells that do not express neutrophil-marking transgenes. Using these lines, the different dynamic behaviors of neutrophils and macrophages after wounding were compared side-by-side in compound transgenics. Macrophage/neutrophil interactions, such as phagocytosis of senescent neutrophils, were readily observed in real time. These zebrafish transgenes provide a new resource that will contribute to the fields of inflammation, infection, and leukocyte biology.

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Cited by

Metadata

Authors
Ellett, F., Pase, L., Hayman, J. W., Andrianopoulos, A., & Lieschke, G. J.
Venue
Blood
Publisher
American Society of Hematology
Published
2011-01-27
Volume
117
Issue
4
Pages
e49-e56
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood-2010-10-314120
OpenAlex
W2050195517
Cited by
1,077
References
3
FWCI
8.73
Type
journal article
Language
en
Open access
green

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