The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Fruit and vegetable consumption and proinflammatory gene expression from peripheral blood mononuclear cells in young adults: a translational study
Open accessAbstract
BACKGROUND: Fruits and vegetables are important sources of fiber and nutrients with a recognized antioxidant capacity, which could have beneficial effects on the proinflammatory status as well as some metabolic syndrome and cardiovascular disease features. The current study assessed the potential relationships of fruit and vegetable consumption with the plasma concentrations and mRNA expression values of some proinflammatory markers in young adults. METHODS: One-hundred and twenty healthy subjects (50 men/70 women; 20.8 +/- 2.6 y; 22.3 +/- 2.8 kg/m2) were enrolled. Experimental determinations included anthropometry, blood pressure and lifestyle features as well as blood biochemical and inflammatory measurements. The mRNA was isolated from peripheral blood mononuclear cells (PBMC) and the gene expression concerning selected inflammatory markers was assessed by quantitative real-time PCR. Nutritional intakes were estimated by a validated semi-quantitative food-frequency questionnaire. RESULTS: The highest tertile of energy-adjusted fruit and vegetable consumption (>660 g/d) was associated with lower plasma concentrations of C-reactive protein (CRP) and homocysteine and with lower ICAM1, IL1R1, IL6, TNFalpha and NFkappaB1 gene expression in PBMC (P for trend < 0.05), independently of gender, age, energy intake, physical activity, smoking, body mass index, systolic blood pressure and circulating non-esterified fatty acids. In addition, plasma CRP, homocysteine and TNFalpha concentrations and ICAM1, TNFalpha and NFkappaB1 gene expression in PBMC showed a descending trend as increased fiber intake (>19.5 g/d) from fruits and vegetables (P for trend < 0.05). Furthermore, the participants within the higher tertile (>11.8 mmol/d) of dietary total antioxidant capacity showed lower plasma CRP and mRNA values of ICAM1, IL1R1, IL6, TNFalpha and NFkappaB1 genes (P for trend < 0.05). The inverse association between fruit and vegetable consumption and study proinflammatory markers followed the same trend and remained statistically significant, after the inclusion of other foods/nutrients in the linear regression models. CONCLUSION: A higher fruit and vegetable consumption was independently associated not only with reduced CRP and homocysteine concentrations but also with a lower mRNA expression in PBMC of some relevant proinflammatory markers in healthy young adults.
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“This was first identified by Grimble et al. (649) who showed that the ability of fish oil to lower the LPS-stimulated production of TNF-a by blood mononuclear cells was determined in part by polymorphisms within the TNF-a and TNF-b genes.”
From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [649]
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Cites
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Livak, K. J., & Schmittgen, T. D. (2001) · Methods · doi:10.1006/meth.2001.1262 · Cited by 180,860 · cited 1×
“Gene expression levels were assessed and normalized by using 18s rRNA as internal control following previously described protocols [26,27].”
MethodMatthews, D. R., Hosker, J. P., Rudenski, A. S., Naylor, B. A., Treacher, D. F., & Turner, R. C. (1985) · Diabetologia · doi:10.1007/bf00280883 · Cited by 31,502 · cited 1×
“Insulin resistance was estimated by the HOMA-IR, which was calculated as follows: HOMA-IR = [fasting glucose (mmol/l) × fasting insulin (μIU/l)]/22.5 [ 24 ].”
MethodInfluentialInflammation and metabolic disorders
Hotamisligil, G. S. (2006) · Nature · doi:10.1038/nature05485 · Cited by 9,199 · cited 1×
“An altered expression of ILs, TNFα, ICAM1 genes and of their respective receptors in adipose tissue as well as in PBMC has been implicated in the higher risk of suffering metabolic syndrome and cardiovascular disease [3,4,35].”
BackgroundA Clinical Trial of the Effects of Dietary Patterns on Blood Pressure
Appel, L. J., Moore, T. J., Obarzanek, E., Vollmer, W. M., Svetkey, L. P., Sacks, F. M., Bray, G. A., Vogt, T. M., Cutler, J. A., Windhauser, M. M., et al. (1997) · New England Journal of Medicine · doi:10.1056/nejm199704173361601 · Cited by 5,981
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Mechanisms linking obesity with cardiovascular disease
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“These changes appear to involve a higher production of proinflammatory and pro-atherogenic molecules such as C-reactive protein (CRP), homocysteine, selectins and adhesion molecules as well as some cytokines [1,3].”
BackgroundInfluentialAdipose tissue dysfunction in obesity, diabetes, and vascular diseases
Hajer, G. R., van Haeften, T. W., & Visseren, F. L. J. (2008) · European Heart Journal · doi:10.1093/eurheartj/ehn387 · Cited by 1,410 · cited 3×
“These changes appear to involve a higher production of proinflammatory and pro-atherogenic molecules such as C-reactive protein (CRP), homocysteine, selectins and adhesion molecules as well as some cytokines [1,3].”
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“Discussion A high intake of fruits and vegetables has been inversely associated with metabolic syndrome features [5,6], cardiovascular diseases [31,32], and total mortality [33], while greater values of proinflammatory markers such Figure 1 Ratios of protein levels of selected proinflammatory markers, calculated from…”
BackgroundDevelopment and Validation of a Food Frequency Questionnaire in Spain
Martin-Moreno, J. M., Boyle, P., Gorgojo, L., Maisonneuve, P., Fernandez‐Rodriguez, J. C., Salvini, S., & Willett, W. C. (1993) · International Journal of Epidemiology · doi:10.1093/ije/22.3.512 · Cited by 909 · cited 2×
“This semi-quantitative food frequency questionnaire, which is validated for Spanish people [16], includes 136 items, considering 13 fruit items (oranges, banana, apple, pear, strawberry, grapefruit, peach/apricot/nectarine, cherry/plum, watermelon, melon, mango, papaya, and kiwi) and 11 vegetable items (spinach, lettu…”
BackgroundCirculating Mononuclear Cells in the Obese Are in a Proinflammatory State
Ghanim, H., Aljada, A., Hofmeyer, D., Syed, T., Mohanty, P., & Dandona, P. (2004) · Circulation · doi:10.1161/01.cir.0000142055.53122.fa · Cited by 611 · cited 2×
“(PBMC) may have an important role in all these complex inflammatory processes, which are mediated by transcriptional nuclear-factors, cytokines and other proatherogenic molecules [4].”
Background
Research works printed but not linked: 1
- Moreiras O, Carbajal A, Cabrera L, Cuadrado C: Tabla de composición de alimentos. 2006, Madrid: Ediciones Pirámide, 10
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Dietary factors and low-grade inflammation in relation to overweight and obesity
Calder, P. C., Ahluwalia, N., Brouns, F., Buetler, T., Clement, K., Cunningham, K., Esposito, K., Jönsson, L. S., Kolb, H., Lansink, M., et al. (2011) · British Journal of Nutrition · doi:10.1017/s0007114511005460 · Cited by 1,098
“A recent study reported that a high intake of vegetables and fruits was associated with a lower peripheral blood mononuclear cell gene expression for several pro-inflammatory cytokines and adhesion molecules((326)).”
Shivappa, N., Steck, S. E., Hurley, T. G., Hussey, J. R., Ma, Y., Ockene, I. S., Tabung, F. K., & Hébert, J. R. (2013) · Public Health Nutrition · doi:10.1017/s1368980013002565 · Cited by 724
“In addition, CRP and inflammatory cytokines such as IL-6 and TNF-a are increased among obese individuals, positively correlated with BMI, and central adiposity has been shown to be associated with increased inflammation(7).”
BackgroundAge-associated chronic diseases require age-old medicine: Role of chronic inflammation
Prasad, S., Sung, B., & Aggarwal, B. B. (2011) · Preventive Medicine · doi:10.1016/j.ypmed.2011.11.011 · Cited by 317
“Fruits and vegetables have been shown to suppress CVD biomarkers, including CRP, in in vitro models (Hermsdorff et al., 2010), animals (Rein et al., 2006), and humans (Franzini et al., 2010).”
BackgroundRuiz‐Canela, M., Zazpe, I., Shivappa, N., Hébert, J. R., Sánchez‐Tainta, A., Corella, D., Salas‐Salvadó, J., Fitó, M., Lamuela-Raventós, R. M., Rekondo, J., et al. (2015) · British Journal Of Nutrition · doi:10.1017/s0007114514004401 · Cited by 285
Virgin olive oil supplementation and long-term cognition: the Predimed-Navarra randomized, trial
Martínez‐Lapiscina, E. H., Clavero, P., Toledo, E., Julian, B. S., Sánchez‐Tainta, A., Corella, D., Lamuela-Raventós, R. M., Martínez, J. A., & Martínez‐González, M. Á. (2013) · The journal of nutrition health & aging · doi:10.1007/s12603-013-0027-6 · Cited by 267
Metadata
- Authors
- Hermsdorff, H. H. M., Zulet, M. Á., Puchau, B., & Martínez, J. A.
- Affiliations
- Universidad de Navarra (ES)
- Corresponding author
- Martínez, José Alfredo
- Venue
- Nutrition & Metabolism
- Published
- 2010-05-13
- Volume
- 7
- Issue
- 1
- Pages
- 42-42
- ISSN
- 1743-7075 (ISSN-L)
- PMC
- PMC2882916
- OpenAlex
- W2161028038
- Cited by
- 141
- References
- 58
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 8 · 2023: 7 · 2022: 4 · 2021: 14 · 2020: 9 · 2019: 11 · 2018: 13 · 2017: 9 · 2016: 6 · 2015: 8 · 2014: 8 · 2013: 13 · 2012: 6
- FWCI
- 11.13
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Nutrition & Metabolism (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Deposito Adademico Digital Universidad De Navarra (University of Navarra) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Universidad de Navarra (LE/97)
- Gobierno de Navarra
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Physiology (medical) › Exercise and Physiological Responses
- Keywords
- fruit and vegetable intake, dietary fiber, pro-inflammatory gene expression, peripheral blood mononuclear cells, C-reactive protein, homocysteine, TNF-α, NFKB1, ICAM-1, IL-1 receptor, interleukin-6, total antioxidant capacity, young adults, inflammation
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