The DOI matches its Crossref record; 41 of the 42 references it cites were found in a registry or an index.
Predominant Enhancement of Glucose Uptake in Astrocytes versus Neurons during Activation of the Somatosensory Cortex
Open accessAbstract
Glucose is the primary energetic substrate of the brain, and measurements of its metabolism are the basis of major functional cerebral imaging methods. Contrary to the general view that neurons are fueled solely by glucose in proportion to their energetic needs, recent in vitro and ex vivo analyses suggest that glucose preferentially feeds astrocytes. However, the cellular fate of glucose in the intact brain has not yet been directly observed. We have used a real-time method for measuring glucose uptake in astrocytes and neurons in vivo in male rats by imaging the trafficking of the nonmetabolizable glucose analog 6-deoxy-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-aminoglucose (6-NBDG) using two-photon microscopy. During resting conditions we found that astrocytes and neurons both take up 6-NBDG at the same rate in the barrel cortex of the rat. However, during intense neuronal activity triggered by whisker stimulation, astrocytes rapidly accelerated their uptake, whereas neuronal uptake remained almost unchanged. After the stimulation period, astrocytes returned to their preactivation rates of uptake paralleling the neuronal rate of uptake. These observations suggest that glucose is taken up primarily by astrocytes, supporting the view that functional imaging experiments based on glucose analogs extraction may predominantly reflect the metabolic activity of the astrocytic network.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The first study (Chuquet et al., 2010) used the fluorescent glucose analogue 6-NBDG; because it cannot be phosphorylated by hexokinase (given that the 6 position was already occupied by the fluorophore), this analogue’s accumulation may not correspond with the actual rate of glucose utilization, and it has been shown in some cases to correlate negatively with glucose utilization (DiNuzzo et al., 2”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Chuquet et al., 2010
In the citing paper
“Two recent in vivo studies monitored glucose analogue accumulation in somatosensory cortex during whisker stimulation; they used two different glucose analogues and obtained opposite results.”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Chuquet et al., 2010
In the citing paper
“The first study (Chuquet et al., 2010) used the fluorescent glucose analogue 6-NBDG; because it cannot be phosphorylated by hexokinase (given that the 6 position was already occupied by the fluorophore), this analogue’s accumulation may not correspond with the actual rate of glucose utilization, and it has been shown in some cases to correlate negatively with glucose utilization (DiNuzzo et al., 2”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Chuquet et al., 2010
In the citing paper
“Baseline accumulation of the analogue into astrocytes and neurons was similar; upon whisker stimulation in anesthetized rats, uptake into both cell types tended to increase, though only significantly for astrocytes (Chuquet et al., 2010).”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Chuquet et al., 2010
In the citing paper
“Baseline accumulation of the analogue into astrocytes and neurons was similar; upon whisker stimulation in anesthetized rats, uptake into both cell types tended to increase, though only significantly for astrocytes (Chuquet et al., 2010).”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Chuquet et al., 2010
In the citing paper
“In vivo measurements to learn which cell type shows greater stimulated uptake of glucose analogs have given conflicting results (Chuquet et al., 2010; Lundgaard et al., 2015)”
From Neuronal Stimulation Triggers Neuronal Glycolysis and Not Lactate Uptake (2017) · cites this paper as Chuquet et al., 2010
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
An Energy Budget for Signaling in the Grey Matter of the Brain
Attwell, D., & Laughlin, S. B. (2001) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-200110000-00001 · Cited by 3,543
Raichle, M. E., & Mintun, M. A. (2006) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.29.051605.112819 · Cited by 1,630
Astroglial Metabolic Networks Sustain Hippocampal Synaptic Transmission
Rouach, N., Koulakoff, A., Abudara, V., Willecke, K., & Giaume, C. (2008) · Science · doi:10.1126/science.1164022 · Cited by 912
Herrero-Mendez, A., Almeida, A., Fernández, E., Maestre, C., Moncada, S., & Bolaños, J. P. (2009) · Nature Cell Biology · doi:10.1038/ncb1881 · Cited by 844
RELATION BETWEEN PHYSIOLOGICAL FUNCTION AND ENERGY METABOLISM IN THE CENTRAL NERVOUS SYSTEM
Sokoloff, L. (1977) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1977.tb03919.x · Cited by 807
Activity-dependent regulation of energy metabolism by astrocytes: an update
Pellerin, L., Bouzier‐Sore, A., Aubert, A., Serres, S., Merle, M., Costalat, R., & Magistretti, P. J. (2007) · Glia · doi:10.1002/glia.20528 · Cited by 805
Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo
Nimmerjahn, A., Kirchhoff, F., Kerr, J. N. D., & Helmchen, F. (2004) · Nature Methods · doi:10.1038/nmeth706 · Cited by 784
Cellular mechanisms of brain energy metabolism and their relevance to functional brain imaging
Magistretti, P. J., & Pellerin, L. (1999) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.1999.0471 · Cited by 750
Astrocyte Responses to CNS Injury
Norenberg, M. D. (1994) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/00005072-199405000-00001 · Cited by 589
Hertz, L., Peng, L., & Dienel, G. A. (2007) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600343 · Cited by 587
Chung, S., Li, X., & Nelson, S. B. (2002) · Neuron · doi:10.1016/s0896-6273(02)00659-1 · Cited by 530 · cited 2×
“sponses to sustained whisker deflection (Chung et al., 2002) we”
BackgroundAlmeida, A., Almeida, J., Bolaños, J. P., & Moncada, S. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.261560998 · Cited by 423
Neuronal-glial glucose oxidation and glutamatergic-GABAergic function.
Hyder, F., Patel, A. B., Gjedde, A., Rothman, D. L., Behar, K. L., & Shulman, R. G. (2006) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600263 · Cited by 408
Patel, A. B., de Graaf, R. A., Mason, G. F., Rothman, D. L., Shulman, R. G., & Behar, K. L. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0501703102 · Cited by 343
Contreras, D., & Steriade, M. (1996) · The Journal of Physiology · doi:10.1113/jphysiol.1996.sp021133 · Cited by 329
Voutsinos‐Porche, B., Bonvento, G., Tanaka, K., Steiner, P., Welker, E., Chatton, J., Magistretti, P. J., & Pellerin, L. (2003) · Neuron · doi:10.1016/s0896-6273(02)01170-4 · Cited by 293
Loaiza, A., Porras, O. H., & Barros, L. F. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-19-07337.2003 · Cited by 254 · cited 2×
“…fluorescent glucose to investigate brain glucose metabolism, Barros and coworkers have shown in vitro that glutamate-induced neuronal firing surprisingly mediates acute inhibition of glucose transport in neurons while stimulating astrocytic glucose uptake (Loaiza et al., 2003; Porras et al., 2004).”
BackgroundSibson, N. R., Mason, G. F., Shen, J., Cline, G. W., Herskovits, A. Z., Wall, J. E. M., Behar, K. L., Rothman, D. L., & Shulman, R. G. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00074.x · Cited by 239
Neuroglial Metabolism in the Awake Rat Brain: CO2 Fixation Increases with Brain Activity
Öz, G., Berkich, D., Henry, P.-G., Xu, Y., Lanoue, K., Hutson, S., & Gruetter, R. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.3564-04.2004 · Cited by 213
Bioenergetics of cerebral ischemia: A cellular perspective
Hertz, L. (2008) · Neuropharmacology · doi:10.1016/j.neuropharm.2008.05.023 · Cited by 196
Yamada, K., Nakata, M., Horimoto, N., Saito, M., Matsuoka, H., & Inagaki, N. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m908048199 · Cited by 191
Mangia, S., Simpson, I. A., Vannucci, S. J., & Carruthers, A. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06003.x · Cited by 189
Schmidt, M. (2009) · Frontiers in Neuroenergetics · doi:10.3389/neuro.14.001.2009 · Cited by 179
Magistretti, P. J., & Pellerin, L. (1996) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1996.tb34449.x · Cited by 159
The redox switch/redox coupling hypothesis
Cerdán, S., Rodrigues, T. B., Sierra, A., Benito, M., Fonseca, L. L., Fonseca, C. P., & García-Martín, M. L. (2006) · Neurochemistry International · doi:10.1016/j.neuint.2005.12.036 · Cited by 146
Glutamate mediates acute glucose transport inhibition in hippocampal neurons
Porras, O. H., Loaiza, A., & Barros, L. F. (2004) · The Journal of Neuroscience · doi:10.1523/jneurosci.1882-04.2004 · Cited by 139
Computer simulations of neuron-glia interactions mediated by ion flux
Somjen, G. G., Kager, H., & Wadman, W. J. (2008) · Journal of Computational Neuroscience · doi:10.1007/s10827-008-0083-9 · Cited by 130
Lopes‐Cardozo, M., Larsson, O. M., & Schousboe, A. (1986) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1986.tb13039.x · Cited by 124
Nehlig, A., Wittendorp-Rechenmann, E., & Dao Lam, C. (2004) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/01.wcb.0000128533.84196.d8 · Cited by 111
Asymmetric transport of a fluorescent glucose analogue by human erythrocytes
Speizer, L. A., Haugland, R. P., & Kutchai, H. C. (1985) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(85)90476-6 · Cited by 100
Cellular pathways of energy metabolism in the brain: Is glucose used by neurons or astrocytes?
Nehlig, A., & Coles, J. A. (2007) · Glia · doi:10.1002/glia.20376 · Cited by 91
The contribution of astrocytes to the 18F-2-deoxyglucose signal in PET activation studies.
Magistretti, P. J., & Pellerin, L. (1996) · PubMed · Cited by 89
Deciphering neuron-glia compartmentalization in cortical energy metabolism
Jolivet, R. (2009) · Frontiers in Neuroenergetics · doi:10.3389/neuro.14.004.2009 · Cited by 89
Cruz, N. F., Lasater, A., Zielke, H. R., & Dienel, G. A. (2005) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2004.02935.x · Cited by 88
Barros, L. F., Courjaret, R. J., Jakoby, P., Loaiza, A., Lohr, C., & Deitmer, J. W. (2008) · Glia · doi:10.1002/glia.20820 · Cited by 83
Determinants of Deoxyglucose Uptake in Cultured Astrocytes: The Role of the Sodium Pump
Brookes, N., & Yarowsky, P. (1985) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1985.tb05438.x · Cited by 77
Fluorometric Determination of Glucose Utilization in Neurons in Vitro and in Vivo
Itoh, Y., Abe, T., Takaoka, R., & Tanahashi, N. (2004) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/01.wcb.0000127661.07591.de · Cited by 72
Wyss, M. T., Weber, B., Treyer, V., Heer, S., Pellerin, L., Magistretti, P. J., & Buck, A. (2008) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2008.86 · Cited by 44
Flow cytometric analysis of glucose transport by rat brain cells
Aller, C. B., Ehmann, S., Gilman‐Sachs, A., & Snyder, A. K. (1997) · Cytometry · doi:10.1002/(sici)1097-0320(19970301)27:3 · Cited by 22
McCasland, J. S. (1996) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(96)00063-5 · Cited by 18
Regional differences in glucose transport in the mouse hippocampus
Shimada, M., Kawamoto, S., Hirose, Y., Nakanishi, M., Watanabe, H., & Watanabe, M. (1994) · The Histochemical Journal · doi:10.1007/bf02388435 · Cited by 15
Cited by
Sorted by citations
Brain energy metabolism: focus on Astrocyte-neuron metabolic cooperation
Bélanger, M., Allaman, I., & Magistretti, P. J. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2011.08.016 · Cited by 2,313
Verkhratsky, A., & Nedergaard, M. (2018) · Physiological Reviews · doi:10.1152/physrev.00042.2016 · Cited by 1,678
Lactate in the brain: from metabolic end-product to signalling molecule
Magistretti, P. J., & Allaman, I. (2018) · Nature Reviews Neuroscience · doi:10.1038/nrn.2018.19 · Cited by 1,298
A Cellular Perspective on Brain Energy Metabolism and Functional Imaging
Magistretti, P. J., & Allaman, I. (2015) · Neuron · doi:10.1016/j.neuron.2015.03.035 · Cited by 1,285
Fong, M. Y., Zhou, W., Liu, L., Alontaga, A. Y., Chandra, M., Ashby, J., Chow, A., O’Connor, S. T. F., Li, S., Chin, A. R., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3094 · Cited by 1,118
Metadata
- Authors
- Chuquet, J., Quilichini, P., Nimchinsky, E. A., & Buzsáki, G.
- Affiliations
- Rutgers, The State University of New Jersey (US)
- Venue
- The Journal of Neuroscience
- Publisher
- Society for Neuroscience
- Published
- 2010-11-10
- Volume
- 30
- Issue
- 45
- Pages
- 15298-15303
- ISSN
- 0270-6474 (ISSN-L)
- 1529-2401
- PMC
- PMC2997269
- OpenAlex
- W2009966509
- Cited by
- 217
- References
- 42
- Citations by year
- 2026: 1 · 2025: 14 · 2024: 13 · 2023: 18 · 2022: 12 · 2021: 15 · 2020: 11 · 2019: 14 · 2018: 13 · 2017: 23 · 2016: 13 · 2015: 19 · 2014: 11 · 2013: 14 · 2012: 14
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by)
- Funders
- National Institute on Deafness and Other Communication Disorders (R01DC009947)
- National Institute of Neurological Disorders and Stroke (R01NS034994)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- astrocytes, neurons, glucose uptake, somatosensory cortex, barrel cortex, whisker stimulation, neuronal activation, two-photon microscopy, brain energy metabolism, functional neuroimaging
- MeSH terms
- Analysis of Variance, Animals, Astrocytes, Biological Transport, Glucose, Male, Neurons, Somatosensory Cortex, Vibrissae, Rats, Sprague-Dawley, Microscopy, Fluorescence, Multiphoton, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
