Cited by 211Muscle Physiology and DisordersNerve injury and regenerationNeurogenetic and Muscular Disorders Research

BDNF is expressed in skeletal muscle satellite cells and inhibits myogenic differentiation

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Mousavi, Kambiz, Jasmin, Bernard J.·2006·The Journal of Neuroscience

Abstract

In skeletal muscle, brain-derived neurotrophic factor (BDNF) has long been thought to serve as a retrograde trophic factor for innervating motor neurons throughout their lifespan. However, its localization in mature muscle fibers has remained elusive. Given the postulated roles of BDNF in skeletal muscle, we performed a series of complementary experiments aimed at defining the localization of BDNF and its transcripts in adult muscle. By reverse transcription-PCR, in situ hybridization, and immunofluorescence, we show that BDNF, along with the receptor p75 NTR , is not expressed at significant levels within mature myofibers and that it does not accumulate preferentially within subsynaptic regions of neuromuscular junctions. Interestingly, expression of BDNF correlated with that of Pax3, a marker of muscle progenitor cells, in several different adult skeletal muscles. Additionally, BDNF was expressed in Pax7+ satellite cells where it colocalized with p75 NTR . In complementary cell culture experiments, we detected high levels of BDNF and p75 NTR in myoblasts. During myogenic differentiation, expression of BDNF became drastically reduced. Using small interfering RNA (siRNA) technology to knock down BDNF expression, we demonstrate enhanced myogenic differentiation of myoblasts. This accelerated rate of myogenic differentiation seen in myoblasts expressing BDNF siRNA was normalized by administration of recombinant BDNF. Collectively, these findings show that BDNF plays an important regulatory function during myogenic differentiation. In addition, the expression of BDNF in satellite cells is coherent with the notion that BDNF serves a key role in maintaining the population of muscle progenitors in adult muscle.

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Crossref · 10.1523/jneurosci.5398-05.2006

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  • BDNF can enter the brain by freely diffusing across the blood brain barrier.

    “BDNF can also enter the brain via freely diffusing across the blood brain barrier (Pan et al., 1998; Mousavi and Jasmin, 2006).”

    From Exercise-Mediated Neurogenesis in the Hippocampus via BDNF (2018) · cites this paper as Mousavi and Jasmin, 2006

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Authors
Mousavi, K., & Jasmin, B. J.
Affiliations
University of Ottawa (CA)
Corresponding author
Jasmin, Bernard J.
Venue
The Journal of Neuroscience
Published
2006-05-24
Volume
26
Issue
21
Pages
5739-5749
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
OpenAlex
W2029048316
Cited by
210
References
58
Citations by year
2026: 10 · 2025: 16 · 2024: 20 · 2023: 9 · 2022: 20 · 2021: 13 · 2020: 11 · 2019: 11 · 2018: 9 · 2017: 11 · 2016: 14 · 2015: 10 · 2014: 10 · 2013: 13 · 2012: 3
FWCI
1.63
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Canadian Institutes of Health Research
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Health Sciences › Medicine › Genetics › Neurogenetic and Muscular Disorders Research
Keywords
brain-derived neurotrophic factor, skeletal muscle satellite cells, myogenesis, p75 neurotrophin receptor, Pax3, Pax7, muscle progenitor cells, myoblasts, neuromuscular junction, muscle regeneration
MeSH terms
Animals, Cell Differentiation, Cells, Cultured, Female, Tissue Distribution, Rats, Sprague-Dawley, Muscle, Skeletal, Brain-Derived Neurotrophic Factor, Receptor, Nerve Growth Factor, Satellite Cells, Skeletal Muscle, Rats

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