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Cited by 45Ruminant Nutrition and Digestive PhysiologyMycotoxins in Agriculture and FoodMicrobial Inactivation Methods

Aerobic deterioration stimulates outgrowth of spore-forming Paenibacillus in corn silage stored under oxygen-barrier or polyethylene films

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Borreani, Giorgio, Dolci, Paola, Tabacco, Ernesto, Cocolin, Luca·2013·Journal of Dairy Science
DOI 10.3168/jds.2013-6649
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Abstract

The occurrence of Bacillus and Paenibacillus spores in silage is of great concern to dairy producers because their spores can survive pasteurization and some strains are capable of subsequently germinating and growing under refrigerated conditions in pasteurized milk. The objectives of this study were to verify the role of aerobic deterioration of corn silage on the proliferation of Paenibacillus spores and to evaluate the efficacy of oxygen-barrier films used to cover silage during fermentation and storage to mitigate these undesirable bacterial outbreaks. The trial was carried out on whole-crop maize (Zea mays L.) inoculated with a mixture of Lactobacillus buchneri, Lactobacillus plantarum, and Enterococcus faecium. A standard polyethylene film and a polyethylene-polyamide film with an enhanced oxygen barrier were used to produce the silage bags for this experiment. The silos were stored indoors at ambient temperature (18 to 22°C) and opened after 110 d. The silage was sampled after 0, 2, 5, 7, 9, and 14 d of aerobic exposure to quantify the growth of endospore-forming bacteria during the exposure of silages to air. Paenibacillus macerans (gram-positive, facultatively anaerobic bacteria) was able to develop during the aerobic exposure of corn silage. This species was present in the herbage at harvesting, together with clostridial spores, and survived ensiling fermentation; it constituted more than 60% of the anaerobic spore formers at silage opening. During silage spoilage, the spore concentration of P. macerans increased to values greater than 7.0 log10 cfu/g of silage. The use of different plastic films to seal silages affected the growth of P. macerans and the number of spores during aerobic exposure of silages. These results indicate that the number of Paenibacillus spores could greatly increase in silage after exposure to air, and that oxygen-barrier films could help to reduce the potential for silage contamination of this important group of milk spoilage microorganisms by delaying the onset of aerobic deterioration.

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  • Borreani and Tabacco (2008) and Borreani et al. (2013) observed an improvement in aerobic stability when OB films were used, as they led to reductions in DM losses and aerobic and anaerobic spores of spoilage microorganisms in peripheral areas of the silo and during aerobic stability tests.

    “Borreani and Tabacco (2008) and Borreani et al. (2013) observed an improvement in aerobic stability when OB films were used, as they led to reductions in DM losses and aerobic and anaerobic spores of spoilage microorganisms in peripheral areas of the silo and during aerobic stability tests.”

    From Silage review: Factors affecting dry matter and quality losses in silages (2018) · cites this paper as Borreani et al. (2013)

  • Improper incorporation of deteriorated feed from the top layers of the silo into the feed mixer could increase contamination of the ration with undesirable microorganisms, such as filamentous fungi, aerobic and anaerobic spores, and harmful mycotoxins.

    “the improper incorporation of deteriorated feed from the top layers of the silo in the feed mixer could (1) increase contamination of the ration with undesirable microorganisms, such as filamentous fungi, aerobic and anaerobic spores (Borreani et al., 2013; Dunière et al., 2013), and harmful mycotoxins (Cavallarin et al., 2011; Wambacq et al., 2016)”

    From Silage review: Factors affecting dry matter and quality losses in silages (2018) · cites this paper as Borreani et al., 2013

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Authors
Borreani, G., Dolci, P., Tabacco, E., & Cocolin, L.
Affiliations
University of Turin (IT)
Corresponding author
Borreani, Giorgio
ORCID
Venue
Journal of Dairy Science
Publisher
American Dairy Science Association
Published
2013-08
Volume
96
Issue
8
Pages
5206-5216
ISSN
0022-0302 (ISSN-L)
DOI
10.3168/jds.2013-6649
OpenAlex
W2039107579
Cited by
45
References
43
Citations by year
2026: 3 · 2024: 6 · 2023: 3 · 2022: 2 · 2021: 5 · 2020: 7 · 2019: 4 · 2018: 5 · 2017: 4 · 2015: 2 · 2014: 4
FWCI
4.25
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Research Information System University of Turin (University of Turin) (submittedVersion)
  • INFM-OAR (INFN Catania) (submittedVersion, other-oa)
Funders
Regione Piemonte
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Keywords
Paenibacillus macerans, aerobic deterioration, corn silage, silage spoilage, oxygen barrier films, polyethylene film, silage storage, spore-forming bacteria, milk spoilage, Lactobacillus buchneri, Enterococcus faecium
MeSH terms
Aerobiosis, Zea mays, Dairying, Fermentation, Silage, Spores, Bacterial, Paenibacillus, Food Storage

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