A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria
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Abstract
Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer.
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Inducible systems facilitate temporal regulation of heterologous protein production.
“These systems facilitate temporal regulation of heterologous protein production [102,103].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [102]
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- Authors
- Kelly, C. L., Taylor, G. M., Hitchcock, A., Torres-Méndez, A., & Heap, J. T.
- Affiliations
- Imperial College London , , South Kensington Campus , , ,
- Imperial College London (GB)
- Corresponding author
- Heap, John T.
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-03-15
- Volume
- 7
- Issue
- 4
- Pages
- 1056-1066
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2773104035
- Cited by
- 80
- References
- 77
- Citations by year
- 2026: 6 · 2025: 11 · 2024: 14 · 2023: 9 · 2022: 11 · 2021: 10 · 2020: 8 · 2019: 10 · 2018: 1
- FWCI
- 4.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- ACS Synthetic Biology (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed (publishedVersion)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
- Northumbria Research Link (Northumbria University) (acceptedVersion)
- Spiral (Imperial College London) (submittedVersion, other-oa)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/M011321/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- Synechocystis sp. PCC 6803, cyanobacteria, inducible promoters, gene expression regulation, photostability, leaky expression, synthetic biology tools
- MeSH terms
- Microorganisms, Genetically-Modified, Binding Sites, Escherichia coli, Light, Plasmids, Promoter Regions, Genetic, Rhamnose, DNA-Directed RNA Polymerases, Sigma Factor, Trans-Activators, Gene Expression Regulation, Bacterial, Genes, Reporter, Escherichia coli Proteins, Synechocystis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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