Effects of Beetroot Juice Supplementation on Performance and Fatigue in a 30-s All-Out Sprint Exercise: A Randomized, Double-Blind Cross-Over Study
Open accessAbstract
As a nitric oxide precursor, beetroot juice (BJ) is known to enhance high-intensity exercise performance (80–100% VO2max) yet its impacts on higher intensity sprint exercise (>100% VO2max) remain to be established. This study sought to examine the effects of BJ supplementation on performance and subsequent fatigue during an all-out sprint exercise. Using a randomized cross-over, double-blind, placebo-controlled design, 15 healthy resistance-trained men (22.4 ± 1.6 years) ingested 70 mL of either BJ or placebo. Three hours later, participants undertook a 30-s all-out Wingate test. Before and after the sprint exercise and at 30 s and 180 s post-exercise, three countermovement jumps (CMJ) were performed and blood lactate samples were obtained. Compared to placebo, BJ consumption improved peak (placebo vs. BJ, 848 ± 134 vs. 881 ± 135 W; p = 0.049) and mean (641 ± 91 vs. 666 ± 100 W; p = 0.023) power output and also reduced the time taken to reach Wpeak in the Wingate test (8.9 ± 1.4 vs. 7.3 ± 0.9 s; p = 0.003). No differences were detected in the fatigue index. In addition, while over time CMJ height and power diminished (ANOVA p < 0.001) and blood lactate levels increased (ANOVA p < 0.001), no supplementation effect was observed. Our findings indicate that while BJ supplementation improved performance at the 30-s cycling sprint, this improvement was not accompanied by differences in fatigue during or after this type of exercise.
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Enhanced skeletal muscle contractile function has been observed during electrically stimulated contractions, and enhanced peak power output has been observed during isokinetic dynamometry and cycling exercise.
“Consistent with the potential for improved weightlifting exercise performance after NO3− supplementation, enhanced skeletal muscle contractile function has been observed during electrically stimulated contractions [95,96,103], and enhanced peak power output has been observed during isokinetic dynamometry [97,123,128] and cycling [45,57,60,129,130,131,132,133] exercise.”
From Effects of Dietary Nitrate Supplementation on Weightlifting Exercise Performance in Healthy Adults: A Systematic Review (2020) · cites this paper as [129]
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Cites
The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics
Lundberg, J. O., Weitzberg, E., & Gladwin, M. T. (2008) · Nature Reviews Drug Discovery · doi:10.1038/nrd2466 · Cited by 2,739 · cited 2× · see citing works
“Around 25% of circulating NO3 is taken up by salivary gland acinar cells in a process facilitated by sialin [8,9].”
BackgroundIOC Consensus Statement: Dietary Supplements and the High-performance Athlete
Maughan, R. J., Burke, L. M., Dvorak, J., Larson-Meyer, D. E., Peeling, P., Phillips, S. M., Rawson, E. S., Walsh, N. P., Garthe, I., Geyer, H., et al. (2018) · British Journal of Sports Medicine · doi:10.1136/bjsports-2018-099027 · Cited by 1,004 · see citing works
Human muscle metabolism during intermittent maximal exercise
Gaitanos, G. C., Williams, C., Boobis, L. H., & Brooks, S. (1993) · Journal of Applied Physiology · doi:10.1152/jappl.1993.75.2.712 · Cited by 967 · see citing works
The countermovement jump to monitor neuromuscular status: A meta-analysis
Claudino, J. G., Cronin, J. B., Mezêncio, B., McMaster, D. T., McGuigan, M. R., Tricoli, V., Amadio, A. C., & Serrão, J. C. (2016) · Journal of science and medicine in sport · doi:10.1016/j.jsams.2016.08.011 · Cited by 702 · see citing works
Biomechanics of Sprint Running
Mero, A. A., Komi, P. V., & Gregor, R. J. (1992) · Sports Medicine · doi:10.2165/00007256-199213060-00002 · Cited by 688 · see citing works
The Relationship Between Aerobic Fitness and Recovery from High Intensity Intermittent Exercise
Tomlin, D. L., & Wenger, H. A. (2001) · Sports Medicine · doi:10.2165/00007256-200131010-00001 · Cited by 655 · see citing works
Higher blood flow and circulating NO products offset high-altitude hypoxia among Tibetans
ERZURUM, S. C., Ghosh, S., Janocha, A. J., Xu, W., Bauer, S. M., Bryan, N. S., Tejero, J., Hemann, C. F., Hille, R., Stuehr, D. J., et al. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0707462104 · Cited by 364 · see citing works
Cormie, P., McBride, J. M., & McCaulley, G. O. (2009) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e3181889324 · Cited by 346 · see citing works
Regulation of skeletal muscle glycogen phosphorylase and PDH during maximal intermittent exercise
Parolin, M. L., Chesley, A., Matsos, M. P., Spriet, L. L., Jones, N. L., & Heigenhauser, G. J. F. (1999) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1999.277.5.e890 · Cited by 334 · see citing works
Ferguson, S. K., Hirai, D. M., Copp, S. W., Holdsworth, C. T., Allen, J. D., Jones, A. M., Musch, T. I., & Poole, D. C. (2013) · The Journal of Physiology · doi:10.1113/jphysiol.2012.243121 · Cited by 333 · see citing works
Hooren, B. V., & Zolotarjova, J. (2017) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0000000000001913 · Cited by 333 · see citing works
Hernández, A., Schiffer, T. A., Ivarsson, N., Cheng, A. J., Bruton, J. D., Lundberg, J. O., Weitzberg, E., & Westerblad, H. (2012) · The Journal of Physiology · doi:10.1113/jphysiol.2012.232777 · Cited by 319 · see citing works
Tiso, M., & Schechter, A. N. (2015) · PLOS ONE · doi:10.1371/journal.pone.0119712 · Cited by 311 · see citing works
Vertical jump coordination: fatigue effects
Rodacki, A. L. F., Fowler, N. E., & Bennett, S. J. (2002) · Medicine & Science in Sports & Exercise · doi:10.1097/00005768-200201000-00017 · Cited by 247 · see citing works
Domínguez, R., Cuenca, E., Maté-Muñoz, J., García-Fernández, P., Serra-Paya, N., Estevan, M., Herreros, P., & Garnacho-Castaño, M. (2017) · Nutrients · doi:10.3390/nu9010043 · Cited by 227 · see citing works
Self-citationEmerging role of nitrite in human biology
Dejam, A., Hunter, C. J., Schechter, A. N., & Gladwin, M. T. (2004) · Blood Cells, Molecules, and Diseases · doi:10.1016/j.bcmd.2004.02.002 · Cited by 221 · see citing works
Factors That Differentiate Acceleration Ability in Field Sport Athletes
Lockie, R. G., Murphy, A. J., Knight, T. J., & de Jonge, X. J. (2011) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0b013e31820d9f17 · Cited by 221 · see citing works
Wylie, L. J., Mohr, M., Krustrup, P., Jackman, S. R., Ermιdis, G., Kelly, J., Black, M. I., Bailey, S. J., Vanhatalo, A., & Jones, A. M. (2013) · European Journal of Applied Physiology · doi:10.1007/s00421-013-2589-8 · Cited by 220 · see citing works
Anaerobic energy provision does not limit Wingate exercise performance in endurance-trained cyclists
Calbet, J. A. L., Fernández, J. A. D. P., Garatachea, N., de Vaca, S. C., & Chavarren, J. (2003) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00128.2002 · Cited by 184 · see citing works
Nitrate Supplementation Enhances the Contractile Properties of Human Skeletal Muscle
HAIDER, G., & FOLLAND, J. P. (2014) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0000000000000351 · Cited by 166 · see citing works
Store and recoil of elastic energy in slow and fast types of human skeletal muscles
Bosco, C., Tihanyi, J., Komi, P. V., et al. (1982) · Acta Physiologica Scandinavica · doi:10.1111/j.1748-1716.1982.tb07152.x · Cited by 165 · see citing works
Effects of beetroot juice supplementation on intermittent high-intensity exercise efforts
Domínguez, R., Maté-Muñoz, J. L., Cuenca, E., García-Fernández, P., Mata-Ordoñez, F., Lozano-Estevan, M. C., Veiga-Herreros, P., da Silva, S. F., & Garnacho-Castaño, M. V. (2018) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-017-0204-9 · Cited by 164 · see citing works
Self-citationCoggan, A. R., Leibowitz, J. L., Kadkhodayan, A., Thomas, D. P., Ramamurthy, S., Spearie, C. A., Waller, S., Farmer, M., & Peterson, L. R. (2015) · Nitric Oxide · doi:10.1016/j.niox.2014.08.014 · Cited by 159 · see citing works
Hoon, M. W., Johnson, N. A., Chapman, P., & Burke, L. M. (2013) · International Journal of Sport Nutrition and Exercise Metabolism · doi:10.1123/ijsnem.23.5.522 · Cited by 157 · see citing works
Why Do People Jump the Way They Do?
Bobbert, M. F., & van Soest, A. (2001) · Exercise and Sport Sciences Reviews · doi:10.1097/00003677-200107000-00002 · Cited by 157 · see citing works
Peri, L., Pietraforte, D., Scorza, G., Napolitano, A., Fogliano, V., & Minetti, M. (2005) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.04.021 · Cited by 154 · see citing works
Fiber Type-Specific Effects of Dietary Nitrate
Jones, A. M., Ferguson, S. K., Bailey, S. J., Vanhatalo, A., & Poole, D. C. (2016) · Exercise and Sport Sciences Reviews · doi:10.1249/jes.0000000000000074 · Cited by 149 · see citing works
Coggan, A. R., Leibowitz, J. L., Spearie, C. A., Kadkhodayan, A., Thomas, D. P., Ramamurthy, S., Mahmood, K., Park, S., Waller, S., Farmer, M., et al. (2015) · Circulation Heart Failure · doi:10.1161/circheartfailure.115.002141 · Cited by 136 · see citing works
Effects of nitric oxide on the contraction of skeletal muscle
Maréchal, G., & Gailly, P. (1999) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050359 · Cited by 135 · see citing works
Dietary nitrate supplementation improves sprint and high-intensity intermittent running performance
Thompson, C., Vanhatalo, A., Jell, H., Fulford, J., Carter, J., Nyman, L., Bailey, S. J., & Jones, A. M. (2016) · Nitric Oxide · doi:10.1016/j.niox.2016.10.006 · Cited by 130 · see citing works
Relationship between the efficiency of muscular work during jumping and the energetics of running
Bosco, C., Montanari, G., Ribacchi, R., Giovenali, P., Latteri, F., Iachelli, G., Faina, M., COLLI, R., Monte, A. D., Rosa, M. L., et al. (1987) · European Journal of Applied Physiology · doi:10.1007/bf00640636 · Cited by 124 · see citing works
Whitfield, J., Ludzki, A., Heigenhauser, G. J. F., Senden, J. M. G., Verdijk, L. B., van Loon, L. J. C., Spriet, L. L., & Holloway, G. P. (2016) · The Journal of Physiology · doi:10.1113/jp270844 · Cited by 115 · see citing works
From Nitrate to Nitric Oxide: The Role of Salivary Glands and Oral Bacteria
Qu, X. M., Wu, Z. F., Pang, B. X., Jin, L. Y., Qin, L. Z., & Wang, S. L. (2016) · Journal of Dental Research · doi:10.1177/0022034516673019 · Cited by 110 · cited 2× · see citing works
“Around 25% of circulating NO3 is taken up by salivary gland acinar cells in a process facilitated by sialin [8,9].”
BackgroundMuscular fatigue in response to different modalities of CrossFit sessions
Maté‐Muñoz, J. L., Hernández-Lougedo, J., Barba-Ruíz, M., García-Fernández, P., Garnacho‐Castaño, M. V., & Domínguez, R. (2017) · PLoS ONE · doi:10.1371/journal.pone.0181855 · Cited by 110 · see citing works
Self-citationAo, J., Vi, K., Zubrzyca, E., et al. (1977) · The Journal of Cell Biology · doi:10.1083/jcb.74.1.287 · Cited by 91 · see citing works
Effects of Beetroot Juice Supplementation on a 30-s High-Intensity Inertial Cycle Ergometer Test
Domínguez, R., Garnacho‐Castaño, M. V., Cuenca, E., García-Fernández, P., Muñoz-González, A., de Jesús, F., Lozano-Estevan, M. D. C., da Silva, S. F., Veiga-Herreros, P., & Maté‐Muñoz, J. L. (2017) · Nutrients · doi:10.3390/nu9121360 · Cited by 84 · see citing works
Self-citationFractional use of anaerobic capacity during a 30- and a 45-s Wingate test
Calbet, J. A. L., Chavarren, J., & Dorado, C. (1997) · European Journal of Applied Physiology · doi:10.1007/s004210050253 · Cited by 81 · see citing works
Increase in Maximal Cycling Power With Acute Dietary Nitrate Supplementation
Rimer, E. G., Peterson, L. R., Coggan, A. R., & Martin, J. C. (2016) · International Journal of Sports Physiology and Performance · doi:10.1123/ijspp.2015-0533 · Cited by 81 · see citing works
Kramer, S. J., Baur, D., Spicer, M. T., Vukovich, M. D., & Ormsbee, M. J. (2016) · Journal of the International Society of Sports Nutrition · doi:10.1186/s12970-016-0150-y · Cited by 80 · see citing works
Creatine supplementation improves sprint performance in male sprinters
Skare, O. ‐., Skadberg, Ø., & Wisnes, A. R. (2001) · Scandinavian Journal of Medicine & Science in Sports · doi:10.1034/j.1600-0838.2001.011002096.x · Cited by 78 · see citing works
Effect of Beetroot Juice Supplementation on Aerobic Response during Swimming
Pinna, M., Roberto, S., Milia, R., Marongiu, E., Olla, S., Loi, A., Migliaccio, G. M., Padulo, J., Orlandi, C., Tocco, F., et al. (2014) · Nutrients · doi:10.3390/nu6020605 · Cited by 76 · see citing works
Tiso, M., & Schechter, A. N. (2015) · PLoS ONE · doi:10.1371/journal.pone.0127490 · Cited by 68 · see citing works
Dietary nitrate-induced increases in human muscle power: High versus low responders
Coggan, A. R., Broadstreet, S. R., Mikhalkova, D., Bole, I., Leibowitz, J. L., Kadkhodayan, A., Park, S., Thomas, D. P., Thies, D., & Peterson, L. R. (2018) · Physiological Reports · doi:10.14814/phy2.13575 · Cited by 66 · see citing works
Jonvik, K. L., Nyakayiru, J., Van Dijk, J. W., Maase, K., Ballak, S. B., Senden, J. M. G., Van Loon, L. J. C., & Verdijk, L. B. (2018) · European Journal of Sport Science · doi:10.1080/17461391.2018.1433722 · Cited by 57 · see citing works
Effect of prior exercise at different pedalling frequencies on maximal power in humans
Beelen, A., & Sargeant, A. J. (1993) · European Journal of Applied Physiology · doi:10.1007/bf01427049 · Cited by 54 · see citing works
Thompson, C., Wylie, L. J., Blackwell, J. R., Fulford, J., Black, M. I., Kelly, J., McDonagh, S. T. J., Carter, J., Bailey, S. J., Vanhatalo, A., et al. (2017) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00909.2016 · Cited by 51 · see citing works
Ishikawa, M., Dousset, E., Avela, J., Kyröläinen, H., Kallio, J., Linnamo, V., Kuitunen, S., Nicol, C., & Komi, P. V. (2006) · European Journal of Applied Physiology · doi:10.1007/s00421-006-0180-2 · Cited by 45 · see citing works
A governing relationship for repetitive muscular contraction
Martin, J. C., Brown, N. A. T., Anderson, F. C., & Spirduso, W. W. (2000) · Journal of Biomechanics · doi:10.1016/s0021-9290(00)00048-8 · Cited by 44 · see citing works
Petrov, K. A., Malomouzh, A. I., Kovyazina, I. V., Krejci, É., Nikitashina, A. D., Proskurina, S. E., Васильевич, З. В., & Nikolsky, E. E. (2012) · European Journal of Neuroscience · doi:10.1111/ejn.12029 · Cited by 39 · see citing works
Understanding the Meaning of Lactate Threshold in Resistance Exercises
Garnacho‐Castaño, M. V., Domínguez, R., & Maté‐Muñoz, J. L. (2015) · International Journal of Sports Medicine · doi:10.1055/s-0035-1548803 · Cited by 39 · see citing works
Self-citationGarnacho‐Castaño, M. V., Domínguez, R., Ruiz-Solano, P., & Maté‐Muñoz, J. L. (2015) · The Journal of Strength and Conditioning Research · doi:10.1519/jsc.0000000000000956 · Cited by 36 · see citing works
Self-citationUnderstanding the Meaning of Lactate Threshold in Resistance Exercises
Garnacho‐Castaño, M. V., Domínguez, R., & Maté‐Muñoz, J. L. (2015) · International Journal of Sports Medicine · doi:10.1055/s-0034-1398495 · Cited by 36 · see citing works
Self-citationThompson, C., Vanhatalo, A., Kadach, S., Wylie, L. J., Fulford, J., Ferguson, S. K., Blackwell, J. R., Bailey, S. J., & Jones, A. M. (2018) · Journal of Applied Physiology · doi:10.1152/japplphysiol.00047.2018 · Cited by 32 · see citing works
Dietary nitrate as modulator of physical performance and cardiovascular health
Kerley, C. P. (2017) · Current Opinion in Clinical Nutrition & Metabolic Care · doi:10.1097/mco.0000000000000414 · Cited by 10 · see citing works
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- Authors
- Cuenca, E., Jodra, P., Pérez-López, A., González-Rodríguez, L. G., Fernandes da Silva, S., Veiga-Herreros, P., & Domínguez, R.
- Affiliations
- Universitat Pompeu Fabra (ES)
- Faculty of Health Sciences, University Alfonso X El Sabio, 28691 Madrid, Spain
- Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Alcalá, 28805 Alcalá de Henares, Spain
- Studies Research Group in Neuromuscular Responses (GEPE N), University of Lavras, 37200-000 Lavras, Brazil
- Universidad Alfonso X el Sabio (ES)
- Universidad Isabel I (ES)
- Corresponding author
- Domínguez, Raúl
- Venue
- Nutrients
- Publisher
- MDPI AG
- Published
- 2018-09-04
- Volume
- 10
- Issue
- 9
- Pages
- 1222
- ISSN
- 2072-6643 (ISSN-L)
- PMC
- PMC6164999
- OpenAlex
- W2888847709
- Cited by
- 76
- References
- 54
- Citations by year
- 2026: 11 · 2025: 9 · 2024: 4 · 2023: 10 · 2022: 10 · 2021: 9 · 2020: 16 · 2019: 6 · 2018: 1
- FWCI
- 10.98
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Nutrients (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Banco Santander
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- beetroot juice, nitric oxide precursor, all-out sprints, Wingate test, countermovement jump, exercise fatigue, output power, blood lactate, high-intensity exercise, resistance-trained men, dietary supplementation
- MeSH terms
- Fruit and Vegetable Juices, Double-Blind Method, Fatigue, Humans, Male, Exercise, Plant Roots, Cross-Over Studies, Lactic Acid, Dietary Supplements, Beta vulgaris, Athletic Performance, Young Adult, Sports Nutritional Physiological Phenomena
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