The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Sleep Deprivation and Activation of Morning Levels of Cellular and Genomic Markers of Inflammation
Abstract
BACKGROUND: Inflammation is associated with increased risk of cardiovascular disorders, arthritis, diabetes mellitus, and mortality. The effects of sleep loss on the cellular and genomic mechanisms that contribute to inflammatory cytokine activity are not known. METHODS: In 30 healthy adults, monocyte intracellular proinflammatory cytokine production was repeatedly assessed during the day across 3 baseline periods and after partial sleep deprivation (awake from 11 pm to 3 am). We analyzed the impact of sleep loss on transcription of proinflammatory cytokine genes and used DNA microarray analyses to characterize candidate transcription-control pathways that might mediate the effects of sleep loss on leukocyte gene expression. RESULTS: In the morning after a night of sleep loss, monocyte production of interleukin 6 and tumor necrosis factor alpha was significantly greater compared with morning levels following uninterrupted sleep. In addition, sleep loss induced a more than 3-fold increase in transcription of interleukin 6 messenger RNA and a 2-fold increase in tumor necrosis factor alpha messenger RNA. Bioinformatics analyses suggested that the inflammatory response was mediated by the nuclear factor kappaB inflammatory signaling system as well as through classic hormone and growth factor response pathways. CONCLUSIONS: Sleep loss induces a functional alteration of the monocyte proinflammatory cytokine response. A modest amount of sleep loss also alters molecular processes that drive cellular immune activation and induce inflammatory cytokines; mapping the dynamics of sleep loss on molecular signaling pathways has implications for understanding the role of sleep in altering immune cell physiologic characteristics. Interventions that target sleep might constitute new strategies to constrain inflammation with effects on inflammatory disease risk.
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“A substantial body of evidence indicates that lower quality or quantity of sleep is linked to poorer physical health,20–22 including elevated risks for cardiovascular disease,23 hypertension,24 and early mortality.25”
From Social Media Use and Its Link to Physical Health Indicators (2022) · cites this paper as [21]
In the citing paper
“Transcriptional pathways are also activated following a night of restricted sleep, with evidence of increaes in transcription of IL-6 and TNF (Irwin and Wang et al, 2006).”
From Sleep health: Reciprocal regulation of sleep and innate immunity (2017) · cites this paper as Irwin and Wang et al, 2006
In the citing paper
“TNF-α levels are most often,82,93,112 but not always,107 unchanged after partial sleep restriction.”
From Interacting epidemics? Sleep curtailment, insulin resistance, and obesity (2012) · cites this paper as [107]
In the citing paper
“Increased plasma levels of IL-6 were reported after partial and total sleep deprivation in most cases,93,106,107,108,112 although two studies only reported a loss of circadian rhythms82,96”
From Interacting epidemics? Sleep curtailment, insulin resistance, and obesity (2012) · cites this paper as [107]
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Metadata
- Authors
- Irwin, M. R.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Irwin, Michael R.
- ORCID
- Irwin, Michael R.
- Venue
- Archives of Internal Medicine
- Publisher
- American Medical Association (AMA)
- Published
- 2006-09-18
- Volume
- 166
- Issue
- 16
- Pages
- 1756
- ISSN
- 0003-9926 (ISSN-L)
- OpenAlex
- W2148230248
- Cited by
- 850
- References
- 44
- Citations by year
- 2026: 30 · 2025: 54 · 2024: 48 · 2023: 45 · 2022: 65 · 2021: 42 · 2020: 41 · 2019: 42 · 2018: 44 · 2017: 41 · 2016: 48 · 2015: 54 · 2014: 36 · 2013: 59 · 2012: 48
- FWCI
- 36.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Archives of Internal Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Alcohol Abuse and Alcoholism (AA13239, R01AA013239)
- National Institute on Aging (AG 18367, R01AG018367)
- National Heart, Lung, and Blood Institute (HL 079955, R01HL079955)
- National Institute of Mental Health (MH-55253, R01MH055253, T32MH019925, T32 MH 19925)
- National Institute on Drug Abuse (R01DA016541, DA 16541)
- National Institute of Allergy and Infectious Diseases (AI 52737, R01AI052737)
- National Center for Research Resources (M-01RR00827, (M01 RR00865), M01RR000865, M01RR000827)
- Topics
- Social Sciences › Psychology › Experimental and Cognitive Psychology › Sleep and Work-Related Fatigue
- Life Sciences › Neuroscience › Cognitive Neuroscience › Sleep and Wakefulness Research
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Keywords
- sleep deprivation, inflammation, monocytes, interleukin-6, TNF-α, inflammatory cytokines, cytokine gene transcription, NF-κB signaling, leukocyte gene expression, immune activation
- MeSH terms
- Adult, Female, Flow Cytometry, Humans, Male, Middle Aged, Monocytes, RNA, Messenger, Sleep Deprivation, Transcription, Genetic, Tumor Necrosis Factor-alpha, Biomarkers, Interleukin-6, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling
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