Cited by 5,766Extracellular vesicles in diseaseCellular transport and secretionNanoparticle-Based Drug Delivery

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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

Théry, Clotilde, Amigorena, Sebastian, Raposo, Graça, Clayton, Aled·2006·Current Protocols in Cell Biology

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Abstract

Exosomes are small membrane vesicles found in cell culture supernatants and in different biological fluids. Exosomes form in a particular population of endosomes, called multivesicular bodies (MVBs), by inward budding into the lumen of the compartment. Upon fusion of MVBs with the plasma membrane, these internal vesicles are secreted. Exosomes possess a defined set of membrane and cytosolic proteins. The physiological function of exosomes is still a matter of debate, but increasing results in various experimental systems suggest their involvement in multiple biological processes. Because both cell‐culture supernatants and biological fluids contain different types of lipid membranes, it is critical to perform high‐quality exosome purification. This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosome preparations.

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Authors: Théry, C., Amigorena, S., Raposo, G., & Clayton, A.
Affiliations: Institut Curie (FR)
Institut Curie Paris France
Cardiff University (GB)
ORCID: Théry, Clotilde
Raposo, Graça
Clayton, Aled
Venue: Current Protocols in Cell Biology
Publisher: Wiley
Published: 2006-03
Volume: 30
Issue: 1
Pages: Unit 3.22-Unit 3.22
ISSN: 1934-2500 (ISSN-L)
1934-2616
DOI: 10.1002/0471143030.cb0322s30
OpenAlex: W1877686344
Cited by: 5,766
References: 30
Citations by year: 2026: 206 · 2025: 356 · 2024: 485 · 2023: 502 · 2022: 558 · 2021: 641 · 2020: 620 · 2019: 521 · 2018: 452 · 2017: 390 · 2016: 301 · 2015: 259 · 2014: 177 · 2013: 106 · 2012: 85
FWCI: 3.54
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 31 d)
Locations: Current Protocols in Cell Biology (publishedVersion)
PubMed (publishedVersion)
ORCA Online Research @Cardiff (Cardiff University) (acceptedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords: exosomes, exosome isolation, multivesicular bodies, cell culture supernatant, biological fluids, exosome characterization, membrane vesicles, exosome function
MeSH terms: Animals, Culture Media, Electrophoresis, Polyacrylamide Gel, Flow Cytometry, Humans, Immunohistochemistry, Ultracentrifugation, Cell Culture Techniques, Secretory Vesicles, Microscopy, Electron, Transmission

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Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

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