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Cited by 227Fatty Acid Research and HealthEdible Oils Quality and AnalysisAntioxidant Activity and Oxidative Stress

Effect of low-to-moderate amounts of dietary fish oil on neutrophil lipid composition and function

Healy, D. A., Wallace, F. A., Miles, E. A., Calder, P. C., Newsholme, P.·2000·Lipids
DOI 10.1007/s11745-000-0583-1
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Abstract

Although essential to host defense, neutrophils are also involved in numerous inflammatory disorders including rheumatoid arthritis. Dietary supplementation with relatively large amounts of fish oil [containing >2.6 g eicosapentaenoic acid (EPA) plus 1.4 g docosahexaenoic acid (DHA) per day] can attenuate neutrophil functions such as chemotaxis and superoxide radical production. In this study, the effects of more moderate supplementation with fish oil on neutrophil lipid composition and function were investigated. The rationale for using lower supplementary doses of fish oil was to avoid adverse gastrointestinal problems, which have been observed at high supplementary concentrations of fish oil. Healthy male volunteers aged <40 yr were randomly assigned to consume one of six dietary supplements daily for 12 wk (n = 8 per treatment group). The dietary supplements included four different concentrations of fish oil (the most concentrated fish oil provided 0.58 g EPA plus 1.67 g DHA per day), linseed oil, and a placebo oil. The percentages of EPA and DHA increased (both P < 0.05) in neutrophil phospholipids in a dose-dependent manner after 4 wk of supplementation with the three most concentrated fish oil supplements. No further increases in EPA or DHA levels were observed after 4 wk. The percentage of arachidonic acid in neutrophil phospholipids decreased (P < 0.05) after 12 wk supplementation with the linseed oil supplement or the two most concentrated fish oil supplements. There were no significant changes in N-formyl-met-leu-phe-induced chemotaxis and superoxide radical production following the dietary supplementations. In conclusion, low-to-moderate amounts of dietary fish oil can be used to manipulate neutrophil fatty acid composition. However, this may not be accompanied by modulation of neutrophil functions such as chemotaxis and superoxide radical production.

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Crossref · 10.1007/s11745-000-0583-1

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  • In diabetic subjects with normal renal function, a low-advanced glycation end product diet for 2 or 6 weeks decreased several inflammatory markers.

    “The findings of this latter study are in accordance with earlier data in diabetic subjects with normal renal function who showed a decrease in several inflammatory markers following 2 or 6 weeks of a low-AGE diet (521) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [521]

  • Pro-inflammatory transcription factors, inflammatory markers including CRP, IL-6, IL-8, TNF-a and matrix metalloproteinase, and endothelial dysfunction markers VCAM-1, ICAM-1 and E-selectin are elevated in response to acute and persistent hyperglycaemia.

    “Accordingly, pro-inflammatory transcription factors, inflammatory markers including CRP, IL-6, IL-8, TNF-a and matrix metalloproteinase and markers of endothelial dysfunction VCAM-1, ICAM-1 and E-selectin have been observed to be elevated in response to acute and persistent hyperglycaemia (474,475,514,521,653,662,670,671) .”

    From Dietary factors and low-grade inflammation in relation to overweight and obesity (2011) · cites this paper as [521]

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Authors
Healy, D. A., Wallace, F. A., Miles, E. A., Calder, P. C., & Newsholme, P.
Affiliations
  • University College Dublin (IE)
  • University of Southampton (GB)
Corresponding author
Newsholme, P.
ORCID
Venue
Lipids
Publisher
Wiley
Published
2000-07
Volume
35
Issue
7
Pages
763-768
ISSN
  • 0024-4201 (ISSN-L)
  • 1558-9307
DOI
10.1007/s11745-000-0583-1
OpenAlex
W2089965961
Cited by
227
References
57
Citations by year
2026: 2 · 2025: 3 · 2024: 8 · 2023: 3 · 2022: 5 · 2021: 10 · 2020: 6 · 2019: 10 · 2018: 7 · 2017: 14 · 2016: 14 · 2015: 9 · 2014: 13 · 2013: 14 · 2012: 16
FWCI
5.32
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Lipids (publishedVersion)
  • PubMed (publishedVersion)
Funders
Enterprise Ireland
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
  • Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Keywords
dietary fish oil, neutrophils, eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, phospholipids, chemotaxis, linseed oil, dose-dependent effects, rheumatoid arthritis, omega-3 fatty acids
MeSH terms
Adult, Chemotaxis, Leukocyte, Dietary Fats, Unsaturated, Docosahexaenoic Acids, Fish Oils, Humans, Lipids, Male, Neutrophils, Superoxides, Time Factors, Eicosapentaenoic Acid, Arachidonic Acid, Dietary Supplements

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