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Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9
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“Once Cas-9 has found a target site with the appropriate PAM, it triggers local DNA melting followed by the formation of RNA-DNA hybrid, but the mechanism of how Cas-9 enzyme melts target DNA sequence was not clearly understood yet.”
From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [14]
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“PAM sequence is a short (2–5 base-pair length) conserved DNA sequence downstream to the cut site and its size varies depending on the bacterial species.”
From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [14]
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“The most commonly used nuclease in the genome-editing tool, Cas-9 protein recognizes the PAM sequence at 5ʹ-NGG-3ʹ (N can be any nucleotide base).”
From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [14]
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“The Cas-9 nuclease makes double-stranded breaks (DSBs) at a site 3 base pair upstream to PAM.14”
From Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing (2021) · cites this paper as [14]
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RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
Genome-scale CRISPR-Cas9 knockout screening in human cells
Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., Heckl, D., Ebert, B. L., Root, D. E., Doench, J. G., et al. (2014) · Science · doi:10.1126/science.1247005 · Cited by 5,234
Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
Qi, L. S., Larson, M. H., Gilbert, L. A., Doudna, J. A., Weissman, J. S., Arkin, A. P., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.02.022 · Cited by 5,016
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Doench, J. G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E. W., Donovan, K. F., Smith, I., Tothova, Z., Wilen, C., Orchard, R., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3437 · Cited by 4,637
CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
Gilbert, L. A., Larson, M. H., Morsut, L., Liu, Z., Brar, G. A., Torres, S. E., Stern-Ginossar, N., Brandman, O., Whitehead, E. H., Doudna, J. A., et al. (2013) · Cell · doi:10.1016/j.cell.2013.06.044 · Cited by 3,696
High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292
CRISPR-Cas systems for editing, regulating and targeting genomes
Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153
Efficient genome editing in zebrafish using a CRISPR-Cas system
Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K. (2013) · Nature Biotechnology · doi:10.1038/nbt.2501 · Cited by 2,937
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Konermann, S., Brigham, M. D., Trevino, A. E., Joung, J., Abudayyeh, O. O., Barcena, C., Hsu, P. D., Habib, N., Gootenberg, J. S., Nishimasu, H., et al. (2015) · Nature · doi:10.1038/nature14136 · Cited by 2,825
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects
Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature · doi:10.1038/nature16526 · Cited by 2,673
Rationally engineered Cas9 nucleases with improved specificity
Slaymaker, I. M., Gao, L., Zetsche, B., Scott, D. A., Yan, W. X., & Zhang, F. (2016) · Science · doi:10.1126/science.aad5227 · Cited by 2,414
Schwank, G., Koo, B.-K., Sasselli, V., Dekkers, J. F., Heo, I., Demircan, T., Sasaki, N., Boymans, S., Cuppen, E., van der Ent, C. K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.11.002 · Cited by 1,424
CCTop: An intuitive, flexible and reliable CRISPR/Cas9 target prediction tool
Stemmer, M., Thumberger, T., del Sol Keyer, M., Wittbrodt, J., & Mateo, J. L. (2015) · PLoS ONE · doi:10.1371/journal.pone.0124633 · Cited by 1,088
Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases
Perez, E. E., Wang, J., Miller, J. C., Jouvenot, Y., Kim, K. A., Liu, O., Wang, N., Lee, G., Bartsevich, V. V., Lee, Y.-L., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt1410 · Cited by 999
Lin, Y., Cradick, T. J., Brown, M. T., Deshmukh, H., Ranjan, P., Sarode, N., Wile, B. M., Vertino, P. M., Stewart, F. J., & Bao, G. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku402 · Cited by 634
CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs
Mandegar, M. A., Huebsch, N., Frolov, E. B., Shin, E., Truong, A., Olvera, M. P., Chan, A. H., Miyaoka, Y., Holmes, K., Spencer, C. I., et al. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.01.022 · Cited by 525
TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis
Ikmi, A., McKinney, S. A., Delventhal, K. M., & Gibson, M. C. (2014) · Nature Communications · doi:10.1038/ncomms6486 · Cited by 165
Research works printed but not linked: 115
- Grissa, 2007, The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats, BMC Bioinf., 8, 172, 10.1186/1471-2105-8-172 · DOI · Paper page
- Jinek, 2012, A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity, Science, 337, 816, 10.1126/science.1225829 · DOI · Paper page
- Townsend, 2009, High-frequency modification of plant genes using engineered zinc-finger nucleases, Nature, 459, 442, 10.1038/nature07845 · DOI
- Wood, 2011, Targeted genome editing across species using ZFNs and TALENs, Science, 333, 307, 10.1126/science.1207773 · DOI
- Moscou, 2009, A simple cipher governs DNA recognition by TAL effectors, Science, 326, 1501, 10.1126/science.1178817 · DOI
- Boch, 2009, Breaking the code of DNA binding specificity of TAL-type III effectors, Science, 326, 1509, 10.1126/science.1178811 · DOI · Paper page
- Harrison, 2014, A CRISPR view of development, Genes Dev., 28, 1859, 10.1101/gad.248252.114 · DOI
- Cradick, 2013, CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity, Nucleic Acids Res., 10.1093/nar/gkt714 · DOI · Paper page
- Hsu, 2014, Development and applications of CRISPR-Cas9 for genome engineering, Cell, 157, 1262, 10.1016/j.cell.2014.05.010 · DOI · Paper page
- Rollins, 2015, Mechanism of foreign DNA recognition by a CRISPR RNA-guided surveillance complex from Pseudomonas aeruginosa, Nucleic Acids Res., 10.1093/nar/gkv094 · DOI
- Heler, 2015, Cas9 specifies functional viral targets during CRISPR-Cas adaptation, Nature, 10.1038/nature14245 · DOI
- Choudhary, 2015, Gene silencing by CRISPR interference in mycobacteria, Nat. Commun., 6, 10.1038/ncomms7267 · DOI
- Ronda, 2015, CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae, Microb. Cell Factories, 14, 97, 10.1186/s12934-015-0288-3 · DOI
- Jakočiūnas, 2015, Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae, Metab. Eng., 28, 213, 10.1016/j.ymben.2015.01.008 · DOI
- Osakabe, 2014, Genome editing with engineered nucleases in plants, Plant Cell Physiol.
- Zhang, 2010, High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases, Proc. Natl. Acad. Sci., 107, 12028, 10.1073/pnas.0914991107 · DOI
- Li, 2013, Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9, Nat. Biotechnol., 31, 688, 10.1038/nbt.2654 · DOI
- Nekrasov, 2013, Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease, Nat. Biotechnol., 31, 691, 10.1038/nbt.2655 · DOI
- Shan, 2013, Targeted genome modification of crop plants using a CRISPR-Cas system, Nat. Biotechnol., 31, 686, 10.1038/nbt.2650 · DOI · Paper page
- Shan, 2014, Genome editing in rice and wheat using the CRISPR/Cas system, Nat. Protoc., 9, 2395, 10.1038/nprot.2014.157 · DOI
- Jiang, 2013, Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice, Nucleic Acids Res., 41, e188, 10.1093/nar/gkt780 · DOI
- Johnson, 2015, Comparative assessments of CRISPR-Cas nucleases' cleavage efficiency in planta, Plant Mol. Biol., 87, 143, 10.1007/s11103-014-0266-x · DOI
- Feng, 2014, Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis, Proc. Natl. Acad. Sci. U. S. A., 111, 4632, 10.1073/pnas.1400822111 · DOI
- Sugano, 2014, CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L, Plant Cell Physiol., 55, 475, 10.1093/pcp/pcu014 · DOI
- Woo, 2015, DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins, Nat. Biotechnol., 10.1038/nbt.3389 · DOI · Paper page
- Holt, 2010, Zinc finger nuclease-mediated CCR5 knockout hematopoietic stem cell transplantation controls HIV-1 in vivo, Nat. Biotechnol., 28, 839, 10.1038/nbt.1663 · DOI · Paper page
- Cong, 2013, Multiplex genome engineering using CRISPR/Cas systems, Science, 339, 819, 10.1126/science.1231143 · DOI · Paper page
- Hou, 2013, Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis, Proc. Natl. Acad. Sci., 110, 15644, 10.1073/pnas.1313587110 · DOI · Paper page
- Ran, 2013, Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity, Cell, 154, 1380, 10.1016/j.cell.2013.08.021 · DOI · Paper page
- Pinder, 2015, Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing, Nucleic Acids Res., 43, 9379, 10.1093/nar/gkv993 · DOI
- Song, 2016, RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency, Nat. Commun., 7, 10548, 10.1038/ncomms10548 · DOI
- Hilton, 2015, Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers, Nat. Biotechnol., 33, 510, 10.1038/nbt.3199 · DOI
- Wang, 2014, Genetic screens in human cells using the CRISPR-Cas9 system, Science, 343, 80, 10.1126/science.1246981 · DOI · Paper page
- Tzelepis, 2015, A crispr/Cas9 drop-out screen identifies genome-wide genetic valnerubilities in acute myeloid leukaemia, Blood, 126, 554, 10.1182/blood.V126.23.554.554 · DOI
- Port, 2015, Systematic evaluation of Drosophila CRISPR tools reveals safe and robust alternatives to autonomous gene drives in basic research, G3 (Bethesda), 5, 1493, 10.1534/g3.115.019083 · DOI · Paper page
- Xu, 2015, A toolkit of CRISPR-based genome editing systems in Drosophila, J. Genet. Genomics, 42, 141, 10.1016/j.jgg.2015.02.007 · DOI
- Ward, 2015, Rendering the intractable more tractable: tools from Caenorhabditis elegans ripe for import into parasitic nematodes, Genetics, 201, 1279, 10.1534/genetics.115.182717 · DOI
- Xu, 2015, The application of CRISPR-Cas9 genome editing in Caenorhabditis elegans, J. Genet. Genomics, 42, 413, 10.1016/j.jgg.2015.06.005 · DOI
- Pelletier, 2015, Mouse genome engineering via CRISPR-Cas9 for study of immune function, Immunity, 42, 18, 10.1016/j.immuni.2015.01.004 · DOI
- Duncker, 2015, Animal and in silico models for the study of sarcomeric cardiomyopathies, Cardiovasc. Res., 105, 439, 10.1093/cvr/cvv006 · DOI
- Horii, 2015, Genome editing using mammalian haploid cells, Int. J. Mol. Sci., 16, 23604, 10.3390/ijms161023604 · DOI
- Skarnes, 2015, Is mouse embryonic stem cell technology obsolete?, Genome Biol., 16, 109, 10.1186/s13059-015-0673-6 · DOI
- Nakade, 2014, Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, Nat. Commun., 5, 5560, 10.1038/ncomms6560 · DOI
- Nakayama, 2013, Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis, Genesis, 51, 835, 10.1002/dvg.22720 · DOI
- Wang, 2015, Targeted gene disruption in Xenopus laevis using CRISPR/Cas9, Cell Biosci., 5, 1, 10.1186/s13578-015-0006-1 · DOI
- Ansai, 2014, Targeted mutagenesis using CRISPR/Cas system in medaka, Biology Open, 3, 362, 10.1242/bio.20148177 · DOI
- Carroll, 2015, The societal opportunities and challenges of genome editing, Genome Biol., 16, 242, 10.1186/s13059-015-0812-0 · DOI
- Wang, 2015, Genome engineering in cattle: recent technological advancements, Chromosom. Res., 23, 17, 10.1007/s10577-014-9452-6 · DOI
- Grobet, 1997, A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle, Nat. Genet., 17, 71, 10.1038/ng0997-71 · DOI · Paper page
- Proudfoot, 2015, Genome edited sheep and cattle, Transgenic Res., 24, 147, 10.1007/s11248-014-9832-x · DOI
- Heo, 2015, CRISPR/Cas9 nuclease-mediated gene knock-in in bovine-induced pluripotent cells, Stem Cells Dev., 24, 393, 10.1089/scd.2014.0278 · DOI · Paper page
- Ni, 2014, Efficient gene knockout in goats using CRISPR/Cas9 system, PLoS One, 9, e106718, 10.1371/journal.pone.0106718 · DOI
- Wang, 2015, Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system, Sci. Rep., 5, 13878, 10.1038/srep13878 · DOI
- Hai, 2014, One-step generation of knockout pigs by zygote injection of CRISPR/Cas system, Cell Res., 24, 372, 10.1038/cr.2014.11 · DOI
- Zhou, 2014, Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer, Cell. Mol. Life Sci., 72, 1175, 10.1007/s00018-014-1744-7 · DOI
- Lai, 2016, Generation of knock-in pigs carrying Oct4-tdTomato reporter through CRISPR/Cas9-mediated genome engineering, PLoS One, 11
- Peng, 2015, Production of human albumin in pigs through CRISPR/Cas9-mediated knockin of human cDNA into swine albumin locus in the zygotes, Sci. Rep., 5, 16705, 10.1038/srep16705 · DOI
- Zhou, 2015, Efficient generation of gene-modified pigs harboring precise orthologous human mutation via CRISPR/Cas9-induced homology-directed repair in zygotes, Hum. Mutat., 37, 110, 10.1002/humu.22913 · DOI
- Honda, 2015, Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9, Exp. Anim., 64, 31, 10.1538/expanim.14-0034 · DOI
- Witte, 2014, Gene inactivation using the CRISPR/Cas9 system in the nematode Pristionchus pacificus, Dev. Genes Evol.
- Perry, 2015, CRISPR/Cas9-mediated genome modification in the mollusc, Crepidula fornicata, Genesis, 53, 237, 10.1002/dvg.22843 · DOI
- Nakanishi, 2014, CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna, PLoS One, 9, 10.1371/journal.pone.0098363 · DOI
- Martin, 2015, CRISPR/Cas9 mutagenesis reveals versatile roles of Hox genes in crustacean limb specification and evolution, Curr. Biol., 26, 14, 10.1016/j.cub.2015.11.021 · DOI
- Wei, 2014, Heritable genome editing with CRISPR/Cas9 in the silkworm, Bombyx mori, PLoS One, 9, e101210, 10.1371/journal.pone.0101210 · DOI
- Li, 2015, Outbred genome sequencing and CRISPR/Cas9 gene editing in butterflies, Nat. Commun., 6, 8212, 10.1038/ncomms9212 · DOI
- Gilles, 2015, Efficient CRISPR-mediated gene targeting and transgene replacement in the beetle Tribolium castaneum, Development, 142, 2832, 10.1242/dev.125054 · DOI
- Kistler, 2015, Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti, Cell Rep., 11, 51, 10.1016/j.celrep.2015.03.009 · DOI
- Gantz, 2015, Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi, Proc. Natl. Acad. Sci., 112, E6736, 10.1073/pnas.1521077112 · DOI
- Hammond, 2015, A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae, Nat. Biotechnol., 34, 1, 10.1038/nbt.3439 · DOI
- Che-Yi Lin, 2015, Genome editing in sea urchin embryos by using a CRISPR/Cas9 system, Dev. Biol., 409, 1
- Stolfi, 2014, Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9, Development, 141, 4115, 10.1242/dev.114488 · DOI
- Square, 2015, CRISPR/Cas9-mediated mutagenesis in the sea lamprey, Petromyzon marinus: a powerful tool for understanding ancestral gene functions in vertebrates, Development, 142, 4180, 10.1242/dev.125609 · DOI
- Aluru, 2015, Targeted mutagenesis of aryl hydrocarbon receptor 2a and 2b genes in Atlantic killifish (Fundulus heteroclitus), Aquat. Toxicol., 158, 192, 10.1016/j.aquatox.2014.11.016 · DOI
- Harel, 2015, A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate, Cell, 160, 1013, 10.1016/j.cell.2015.01.038 · DOI · Paper page
- Flowers, 2014, Highly efficient targeted mutagenesis in axolotl using Cas9 RNA-guided nuclease, Development, 141, 2165, 10.1242/dev.105072 · DOI
- Hwang, 2013, Heritable and precise zebrafish genome editing using a CRISPR-Cas system, PLoS One, 8, e68708, 10.1371/journal.pone.0068708 · DOI
- Chang, 2013, Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos, Cell Res., 23, 465, 10.1038/cr.2013.45 · DOI
- Jao, 2013, Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system, Proc. Natl. Acad. Sci., 110, 13904, 10.1073/pnas.1308335110 · DOI · Paper page
- Hruscha, 2013, Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish, Development, 140, 4982, 10.1242/dev.099085 · DOI
- Irion, 2014, Precise and efficient genome editing in zebrafish using the CRISPR/Cas9 system, Development, 141, 4827, 10.1242/dev.115584 · DOI
- Xiao, 2013, Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish, Nucleic Acids Res., 10.1093/nar/gkt464 · DOI
- Ablain, 2015, A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish, Dev. Cell, 32, 756, 10.1016/j.devcel.2015.01.032 · DOI
- Yin, 2015, Multiplex conditional mutagenesis using transgenic expression of Cas9 and sgRNAs, Genetics, 200, 431, 10.1534/genetics.115.176917 · DOI
- Shah, 2015, Rapid reverse genetic screening using CRISPR in zebrafish, Nat. Methods, 12, 535, 10.1038/nmeth.3360 · DOI
- Varshney, 2015, High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9, Genome Res., 25, 1030, 10.1101/gr.186379.114 · DOI
- Long, 2015, Regulation of transcriptionally active genes via the catalytically inactive Cas9 in C. elegans and D. rerio, Cell Res., 25, 638, 10.1038/cr.2015.35 · DOI
- Auer, 2014, Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair, Genome Res., 24, 142, 10.1101/gr.161638.113 · DOI · Paper page
- Kimura, 2014, Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering, Sci. Rep., 4, 6545, 10.1038/srep06545 · DOI
- Li, 2015, Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system, Cell Res., 25, 634, 10.1038/cr.2015.43 · DOI
- Hisano, 2015, Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, Sci. Rep., 5, 8841, 10.1038/srep08841 · DOI
- Hoshijima, 2016, Precise editing of the zebrafish genome made simple and efficient, Dev. Cell, 36, 654, 10.1016/j.devcel.2016.02.015 · DOI
- Prykhozhij, 2015, CRISPR MultiTargeter: a web tool to find common and unique CRISPR single guide RNA targets in a set of similar sequences, PLoS One, 10
- Prykhozhij, 2016, A guide to computational tools and design strategies for genome editing experiments in zebrafish using CRISPR/Cas9, Zebrafish, 13, 70, 10.1089/zeb.2015.1158 · DOI
- Doench, 2014, Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation, Nat. Biotechnol., 32, 1262, 10.1038/nbt.3026 · DOI · Paper page
- Xu, 2015, Sequence determinants of improved CRISPR sgRNA design, Genome Res., 10.1101/gr.191452.115 · DOI · Paper page
- Moreno-Mateos, 2015, CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo, Nat. Methods, 10.1038/nmeth.3543 · DOI · Paper page
- Wong, 2015, WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system, Genome Biol., 16, 218, 10.1186/s13059-015-0784-0 · DOI
- Chari, 2015, Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach, Nat. Methods, 12, 1, 10.1038/nmeth.3473 · DOI
- Heigwer, 2014, E-CRISP: fast CRISPR target site identification, Nat. Methods, 11, 122, 10.1038/nmeth.2812 · DOI
- Lei, 2014, CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants, Mol. Plant, 7, 1494, 10.1093/mp/ssu044 · DOI
- Naito, 2014, CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites, Bioinformatics
- Montague, 2014, CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing, Nucleic Acids Res., 10.1093/nar/gku410 · DOI
- Guo, 2015, Online high-throughput mutagenesis designer using scoring matrix of sequence-specific endonucleases, J. Integr. Bioinform., 12, 283, 10.1515/jib-2015-283 · DOI
- Xie, 2014, sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites, PLoS One, 9, e100448, 10.1371/journal.pone.0100448 · DOI
- Zhu, 2014, CRISPRseek: a bioconductor package to identify target-specific guide RNAs for CRISPR-Cas9 genome-editing systems, PLoS One, 9, e108424, 10.1371/journal.pone.0108424 · DOI
- Bae, 2014, Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases, Bioinformatics, 30, 1473, 10.1093/bioinformatics/btu048 · DOI · Paper page
- Xiao, 2014, CasOT: a genome-wide Cas9/gRNA off-target searching tool, Bioinformatics, 10.1093/bioinformatics/btt764 · DOI · Paper page
- O'Brien, 2014, GT-scan: identifying unique genomic targets, Bioinformatics, 30, 2673, 10.1093/bioinformatics/btu354 · DOI
- Guilinger, 2014, Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification, Nat. Biotechnol., 32, 577, 10.1038/nbt.2909 · DOI · Paper page
- Tsai, 2014, Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing, Nat. Biotechnol., 32, 569, 10.1038/nbt.2908 · DOI · Paper page
- Ledford, 2015, CRISPR, the disruptor, Nature, 522, 20, 10.1038/522020a · DOI
- Webber, 2015, Opinion: is CRISPR-based gene drive a biocontrol silver bullet or global conservation threat?, Proc. Natl. Acad. Sci., 112, 10565, 10.1073/pnas.1514258112 · DOI
- Ma, 2013, A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes, BioMed Res. Int., 2013, 270805, 10.1155/2013/270805 · DOI
- Liu, 2015, CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation, Bioinformatics, 31, 3676, 10.1093/bioinformatics/btv423 · DOI
- Ran, 2013, Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity, Cell, 154, 1380, 10.1016/j.cell.2013.08.021 · DOI · Paper page
Cited by
Sorted by citations
Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing
Mengstie, M. A., & Wondimu, B. Z. (2021) · Biologics: Targets and Therapy · doi:10.2147/btt.s326422 · Cited by 478
Metadata
- Authors
- Ceasar, S. A., Rajan, V., Prykhozhij, S. V., Berman, J. N., & Ignacimuthu, S.
- ORCID
- Ignacimuthu, S.
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 1863
- Issue
- 9
- Pages
- 2333-2344
- ISSN
- 0167-4889
- Cited by
- 151
- References
- 19
- Language
- en
- Licence
- Funders
- India-Loyola College (7LCTOI14ERI001)
- King Saud University (ISPP#0020)
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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