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Cited by 223Microbial Metabolites in Food BiotechnologyTryptophan and brain disordersProbiotics and Fermented Foods

Prebiotic administration normalizes lipopolysaccharide (LPS)-induced anxiety and cortical 5-HT2A receptor and IL1-β levels in male mice

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Savignac, Helene M., Couch, Yvonne, Stratford, Michael, Bannerman, David M., Tzortzis, George, Anthony, Daniel C., Burnet, Philip W.J.·2016·Brain, Behavior, and Immunity
DOI 10.1016/j.bbi.2015.10.007PMC PMC4927692
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Abstract

The manipulation of the enteric microbiota with specific prebiotics and probiotics, has been shown to reduce the host's inflammatory response, alter brain chemistry, and modulate anxiety behaviour in both rodents and humans. However, the neuro-immune and behavioural effects of prebiotics on sickness behaviour have not been explored. Here, adult male CD1 mice were fed with a specific mix of non-digestible galacto-oligosaccharides (Bimuno®, BGOS) for 3 weeks, before receiving a single injection of lipopolysaccharide (LPS), which induces sickness behaviour and anxiety. Locomotor and marble burying activities were assessed 4h after LPS injection, and after 24h, anxiety in the light-dark box was assessed. Cytokine expression, and key components of the serotonergic (5-Hydroxytryptamine, 5-HT) and glutamatergic system were evaluated in the frontal cortex to determine the impact of BGOS administration at a molecular level. BGOS-fed mice were less anxious in the light-dark box compared to controls 24h after the LPS injection. Elevated cortical IL-1β concentrations in control mice 28 h after LPS were not observed in BGOS-fed animals. This significant BGOS×LPS interaction was also observed for 5HT2A receptors, but not for 5HT1A receptors, 5HT, 5HIAA, NMDA receptor subunits, or other cytokines. The intake of BGOS did not influence LPS-mediated reductions in marble burying behaviour, and its effect on locomotor activity was equivocal. Together, our data show that the prebiotic BGOS has an anxiolytic effect, which may be related to the modulation of cortical IL-1β and 5-HT2A receptor expression. Our data suggest a potential role for prebiotics in the treatment of neuropsychiatric disorders where anxiety and neuroinflammation are prominent clinical features.

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  • B-GOS administration in mice attenuated post-inflammatory anxiety.

    “However, administration of B-GOS® in mice attenuated post-inflammatory anxiety (Savignac et al., 2016).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Savignac et al., 2016

  • B-GOS prevented LPS-mediated increase in cortical IL-1β and 5-HT 2A receptor levels.

    “In addition, B-GOS® prevented a lipopolysaccharide (LPS)-mediated increase in cortical 1L-1β and 5-HT 2A receptor levels (Savignac et al., 2016).”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Savignac et al., 2016

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Research works printed but not linked: 67

  1. Andrikopoulos S. Blair A.R. Deluca N. Fam B.C. Proietto J. Evaluating the glucose tolerance test in mice Am. J. Physiol. Endocrinol. Metab. 295 2008 E1323 E1332 18812462
  2. Bassi G.S. Kanashiro A. Santin F.M. de Souza G.E. Nobre M.J. Coimbra N.C. Lipopolysaccharide-induced sickness behaviour evaluated in different models of anxiety and innate fear in rats Basic Clin. Pharmacol. Toxicol. 110 2012 359 369 22059515
  3. Bercik P. Verdu E.F. Foster J.A. Macri J. Potter M. Huang X. Malinowski P. Jackson W. Blennerhassett P. Neufeld K.A. Lu J. Khan W.I. Corthesy-Theulaz I. Cherbut C. Bergonzelli G.E. Collins S.M. Chronic gastrointestinal inflammation induces anxiety-like behavior and alters central nervous system biochemistry in mice Gastroenterology 139 2010 2102 2112 20600016
  4. Biesmans S. Meert T.F. Bouwknecht J.A. Acton P.D. Davoodi N. De Haes P. Kuijlaars J. Langlois X. Matthews L.J. Ver Donck L. Hellings N. Nuydens R. Systemic immune activation leads to neuroinflammation and sickness behavior in mice Mediators Inflamm. 2013 2013 271359 23935246 PMC3723093
  5. Bindels L.B. Porporato P. Dewulf E.M. Verrax J. Neyrinck A.M. Martin J.C. Scott K.P. Buc Calderon P. Feron O. Muccioli G.G. Sonveaux P. Cani P.D. Delzenne N.M. Gut microbiota-derived propionate reduces cancer cell proliferation in the liver Br. J. Cancer 107 8 2012 1337 1344 22976799 PMC3494429
  6. Bluthé R.M. Beaudu C. Kelley K.W. Dantzer R. Differential effects of IL-1ra on sickness behavior and weight loss induced by IL-1 in rats Brain Res. 677 1995 171 176 7606464
  7. Bravo J.A. Forsythe P. Chew M.V. Escaravage E. Savignac H.M. Dinan T.G. Bienenstock J. Cryan J.F. Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve Proc. Natl. Acad. Sci. USA 108 2011 16050 16055 21876150 PMC3179073
  8. Burnet P.W. Cowen P.J. Psychobiotics highlight the pathways to happiness Biol. Psychiatry 74 2013 708 709 24144322
  9. Chio C.C. Tsai S.M. Wang J.J. Lin M.T. 5-HT2A-mu opioid receptor mechanisms in the hypothalamus mediate interleukin-1beta fever in rats Neurosci. Lett. 381 2005 6 11 15882780
  10. Chu H. Mazmanian S.K. Innate immune recognition of the microbiota promotes host microbial symbiosis Nat. Immunol. 14 2013 668 675 23778794 PMC4109969
  11. Cirulli F. De Acetis L. Alleva E. Behavioral effects of peripheral interleukin-1 administration in adult CD-1 mice: specific inhibition of the offensive components of intermale agonistic behavior Brain Res. 791 1998 308 312 9593959
  12. Couch Y. Martin C.J. Howarth C. Raley J. Khrapitchev A.A. Stratford M. Sharp T. Sibson N.R. Anthony D.C. Systemic inflammation alters central 5-HT function as determined by pharmacological MRI Neuroimage 275 2013 177 186 23473937 PMC4004801
  13. Couch Y. Xie Q. Lundberg L. Sharp T. Anthony D.C. A model of post-infection fatigue is associated with increased TNF and 5-HT2A receptor expression in mice PLoS ONE 10 2015 e0130643 26147001 PMC4493081
  14. Cryan J.F. Dinan T.G. Mind-altering microorganisms: the impact of the gut microbiota on brain and behaviour Nat. Rev. Neurosci. 13 2012 701 712 22968153
  15. Cunningham C. Campion S. Lunnon K. Murray C.L. Woods J.F. Deacon R.M. Rawlins J.N. Perry V.H. Systemic inflammation induces acute behavioral and cognitive changes and accelerates neurodegenerative disease Biol. Psychiatry 65 2009 304 312 18801476 PMC2633437
  16. Dai Y. Dai D. Wang X. Ding Z. Mehta J.L. DPP-4 inhibitors repress NLRP3 inflammasome and interleukin-1beta via GLP-1 receptor in macrophages through protein kinase C pathway Cardiovasc. Drugs Ther. 28 2014 425 432 25022544
  17. Damsch S. Eichenbaum G. Tonelli A. Lammens L. Van den Bulck K. Feyen B. Vandenberghe J. Megens A. Knight E. Kelley M. Gavage-related reflux in rats: identification, pathogenesis, and toxicological implications Toxicol. Pathol. 39 2011 348 360 21422261
  18. Deacon R.M. Digging and marble burying in mice: simple methods for in vivo identification of biological impacts Nat. Protoc. 1 1 2006 122 124 17406223
  19. de Pablos R.M. Villaran R.F. Arguelles S. Herrera A.J. Venero J.L. Ayala A. Cano J. Machado A. Stress increases vulnerability to inflammation in the rat prefrontal cortex J. Neurosci. 26 2006 5709 5719 16723527 PMC6675274
  20. Dinan T.G. Stanton C. Cryan J.F. Psychobiotics: a novel class of psychotropic Biol. Psychiatry 74 2013 720 726 23759244
  21. Erny D. Hrabě de Angelis A.L. Jaitin D. Wieghofer P. Staszewski O. David E. Keren-Shaul H. Mahlakoiv T. Jakobshagen K. Buch T. Schwierzeck V. Utermöhlen O. Chun E. Garrett W.S. McCoy K.D. Diefenbach A. Staeheli P. Stecher B. Amit I. Prinz M. Host microbiota constantly control maturation and function of microglia in the CNS Nat. Neurosci. 18 2015 965 977 26030851 PMC5528863
  22. Forsythe P. Kunze W.A. Voices from within: gut microbes and the CNS Cell. Mol. Life Sci. 70 2013 55 69 22638926 PMC11113561
  23. Fritz J.H. Rojas O.L. Simard N. McCarthy D.D. Hapfelmeier S. Rubino S. Robertson S.J. Larijani M. Gosselin J. Ivanov I.I. Martin A. Casellas R. Philpott D.J. Girardin S.E. McCoy K.D. Macpherson A.J. Paige C.J. Gommerman J.L. Acquisition of a multifunctional IgA+ plasma cell phenotype in the gut Nature 481 2011 199 203 22158124 PMC3487691
  24. Garcia-Oscos F. Peña D. Housini M. Cheng D. Lopez D. Borland M.S. Salgado-Delgado R. Salgado H. D’Mello S. Kilgard M.P. Rose-John S. Atzori M. Vagal nerve stimulation blocks interleukin 6-dependent synaptic hyperexcitability induced by lipopolysaccharide-induced acute stress in the rodent prefrontal cortex Brain Behav. Immun. 43 2015 149 158 25128387 PMC4727901
  25. Gareau M.G. Jury J. MacQueen G. Sherman P.M. Perdue M.H. Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation Gut 56 2007 1522 1528 17339238 PMC2095679
  26. Gibb J. Hayley S. Poulter M.O. Anisman H. Effects of stressors and immune activating agents on peripheral and central cytokines in mouse strains that differ in stressor responsivity Brain Behav. Immun. 25 2011 468 482 21093579
  27. Griebel G. Perrault G. Sanger D.J. A comparative study of the effects of selective and non selective 5-HT2 receptor subtype antagonists in rat and mouse models of anxiety Neuropharmacology 36 6 1997 793 802 9225307
  28. Gueimonde M. Sánchez B. Enhancing probiotic stability in industrial processes Microb. Ecol. Health Dis. 23 2012 23 (Jun 18) PMC3747747 23990824
  29. Hardy H. Harris J. Lyon E. Beal J. Foey A.D. Probiotics, prebiotics and immunomodulation of gut mucosal defences: homeostasis and immunopathology Nutrients 5 2013 1869 1912 23760057 PMC3725482
  30. Harré E.M. Galic M.A. Mouihate A. Noorbakhsh F. Pittman Q.J. Neonatal inflammation produces selective behavioural deficits and alters N-methyl-D-aspartate receptor subunit mRNA in the adult rat brain Eur. J. Neurosci. 27 3 2008 644 653 18279317 PMC3547975
  31. Jacobson L.H. Cryan J.F. Differential sensitivity to the motor and hypothermic effects of the GABA B receptor agonist baclofen in various mouse strains Psychopharmacology 179 2005 688 699 15668819
  32. Kanauchi O. Andoh A. Mitsuyama K. Effects of the modulation of microbiota on the gastrointestinal immune system and bowel function J. Agric. Food Chem. 61 2013 9977 9983 24070265
  33. Konieczna P. Akdis C.A. Quigley E.M. Shanahan F. O’Mahony L. Portrait of an immunoregulatory Bifidobacterium Gut. Microbes. 3 2012 261 266 22572827 PMC3427218
  34. Ma J. Choi B.R. Chung C. Min S.S. Jeon W.K. Han J.S. Chronic brain inflammation causes a reduction in GluN2A and GluN2B subunits of NMDA receptors and an increase in the phosphorylation of mitogen-activated protein kinases in the hippocampus Mol. Brain. 24 7 2014 33 24761931 PMC4021635
  35. MacDonald L. Hazi A. Paolini A.G. Kent S. Calorie restriction dose-dependently abates lipopolysaccharide-induced fever, sickness behavior, and circulating interleukin-6 while increasing corticosterone Brain Behav. Immun. 40 2014 18 26 24440143
  36. Mackey D. McFall A.J. MAMPs and MIMPs: proposed classifications for inducers of innate immunity Mol. Microbiol. 61 2006 1365 1371 16899081
  37. Marsden W.N. Stressor-induced NMDAR dysfunction as a unifying hypothesis for the aetiology, pathogenesis and comorbidity of clinical depression Med. Hypotheses 77 2011 508 528 21741771
  38. McCarty M.F. DiNicolantonio J.J. Acarbose, lente carbohydrate, and prebiotics promote metabolic health and longevity by stimulating intestinal production of GLP-1 Open Heart. 2 2015 e000205 25685364 PMC4316590
  39. Mitchell A.J. Dinan T.G. Schizophrenia: a multisystem disease? J. Psychopharmacol. 24 2010 5 7 20923915 PMC2951587
  40. Mocci G. Jiménez-Sánchez L. Adell A. Cortés R. Artigas F. Expression of 5-HT2A receptors in prefrontal cortex pyramidal neurons projecting to nucleus accumbens. Potential relevance for atypical antipsychotic action Neuropharmacology 79 2014 49 58 24211653
  41. Neufeld K.M. Kang N. Bienenstock J. Foster J.A. Reduced anxiety-like behavior and central neurochemical change in germ-free mice Neurogastroenterol. Motil. 23 2011 255–264, e119 21054680
  42. Nguyen A.T. Aerts T. Van Dam D. De Deyn P.P. Biogenic amines and their metabolites in mouse brain tissue: development, optimization and validation of an analytical HPLC method J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 878 29 2010 3003 3014 20934393
  43. Nic Dhonnchadha B.A. Bourin M. Hascoët M. Anxiolytic-like effects of 5-HT2 ligands on three mouse models of anxiety Behav. Brain Res. 140 2003 203 214 12644293
  44. Nikodemova M. Watters J.J. Outbred ICR/CD1 mice display more severe neuroinflammation mediated by microglial TLR4/CD14 activation than inbred C57Bl/6 mice Neuroscience 190 2011 67 74 21683771 PMC3156380
  45. Njung’e K. Handley S.L. Evaluation of marble-burying behavior as a model of anxiety Pharmacol. Biochem. Behav. 38 1991 63 67 2017455
  46. Ohgi Y. Futamura T. Kikuchi T. Hashimoto K. Effects of antidepressants on alternations in serum cytokines and depressive-like behavior in mice after lipopolysaccharide administration Pharmacol. Biochem. Behav. 103 4 2013 853 859 23262300
  47. O’Sullivan E. Barrett E. Grenham S. Fitzgerald P. Stanton C. Ross R.P. Quigley E.M. Cryan J.F. Dinan T.G. BDNF expression in the hippocampus of maternally separated rats: does Bifidobacterium breve 6330 alter BDNF levels? Benef. Microbes 2 2011 199 207 21986359
  48. Pan Y. Chen X.Y. Zhang Q.Y. Kong L.D. Microglial NLRP3 inflammasome activation mediates IL-1β-related inflammation in prefrontal cortex of depressive rats Brain Behav. Immun. 41 2014 90 100 24859041
  49. Prinz M. Kann O. Draheim H.J. Schumann R.R. Kettenmann H. Weber J.R. Hanisch U.K. Microglial activation by components of gram-positive and -negative bacteria: distinct and common routes to the induction of ion channels and cytokines J. Neuropathol. Exp. Neurol. 58 1999 1078 1089 10515231
  50. Rashid S.K. Khodja N.I. Auger C. Alhosin M. Boehm N. Oswald-Mammosser M. Schini-Kerth V.B. Probiotics (VSL#3) prevent endothelial dysfunction in rats with portal hypertension: role of the angiotensin system PLoS ONE 9 5 2014 e97458 24832090 PMC4022585
  51. Rook G.A. Raison C.L. Lowry C.A. Microbiota, immunoregulatory old friends and psychiatric disorders Adv. Exp. Med. Biol. 817 2014 319 356 24997041
  52. Savignac H.M. Hyland N.P. Dinan T.G. Cryan J.F. The effects of repeated social interaction stress on behavioural and physiological parameters in a stress-sensitive mouse strain Behav. Brain Res. 216 2011 576 584 20826188
  53. Savignac H.M. Tramullas M. Kiely B. Dinan T.G. Cryan J.F. Bifidobacteria modulate cognitive processes in an anxious mouse strain Behav. Brain Res. 287 2015 59 72 25794930
  54. Schmidt K. Cowen P.J. Harmer C.J. Tzortzis G. Errington S. Burnet P.W. Prebiotic intake reduces the waking cortisol response and alters emotional bias in healthy volunteers Psychopharmacology 232 2015 1793 1801 25449699 PMC4410136
  55. Silk D.B. Davis A. Vulevic J. Tzortzis G. Gibson G.R. Clinical trial: the effects of a trans-galactooligosaccharide prebiotic on faecal microbiota and symptoms in irritable bowel syndrome Aliment. Pharmacol. Ther. 29 2009 508 518 19053980
  56. Skelly D.T. Hennessy E. Dansereau M.A. Cunningham C. A systematic analysis of the peripheral and CNS effects of systemic LPS, IL-1Beta, TNF-alpha and IL-6 challenges in C57BL/6 mice PLoS ONE 8 2013 e69123 23840908 PMC3698075
  57. Stiverson J. Williams T. Chen J. Adams S. Hustead D. Price P. Guerrieri J. Deacon J. Yu Z. Prebiotic oligosaccharides: comparative evaluation using in vitro cultures of infants’ fecal microbiomes Appl. Environ. Microbiol. 80 2014 7388 7397 25239906 PMC4249200
  58. Strekalova T. Spanagel R. Bartsch D. Henn F.A. Gass P. Stress-induced anhedonia in mice is associated with deficits in forced swimming and exploration Neuropsychopharmacology 29 2004 2007 2017 15266352
  59. Sudo N. Chida Y. Aiba Y. Sonoda J. Oyama N. Yu X.N. Kubo C. Koga Y. Postnatal microbial colonization programs the hypothalamic-pituitary-adrenal system for stress response in mice J. Physiol. 558 2004 263 275 15133062 PMC1664925
  60. Tanaka S. Kondo H. Kanda K. Ashino T. Nakamachi T. Sekikawa K. Iwakura Y. Shioda S. Numazawa S. Yoshida T. Involvement of interleukin-1 in lipopolysaccaride-induced microglial activation and learning and memory deficits J. Neurosci. Res. 89 4 2011 506 514 21290410
  61. Tzortzis G. Goulas A.K. Gee J.M. Gibson G.R. A novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo J. Nutr. 135 2005 1726 1731 15987856
  62. Viviani B. Gardoni F. Bartesaghi S. Corsini E. Facchi A. Galli C.L. Di Luca M. Marinovich M. Interleukin-1 beta released by gp120 drives neural death through tyrosine phosphorylation and trafficking of NMDA receptors J. Biol. Chem. 281 40 2006 30212 30222 16887807
  63. Vulevic J. Drakoularakou A. Yaqoob P. Tzortzis G. Gibson G.R. Modulation of the fecal microflora profile and immune function by a novel trans-galactooligosaccharide mixture (B-GOS) in healthy elderly volunteers Am. J. Clin. Nutr. 88 2008 1438 1446 18996881
  64. Weisstaub N.V. Zhou M. Lira A. Lambe E. González-Maeso J. Hornung J.P. Sibille E. Underwood M. Itohara S. Dauer W.T. Ansorge M.S. Morelli E. Mann J.J. Toth M. Aghajanian G. Sealfon S.C. Hen R. Gingrich J.A. Cortical 5-HT2A receptor signaling modulates anxiety-like behaviors in mice Science 313 2006 536 540 16873667
  65. Wohleb E.S. Fenn A.M. Pacenta A.M. Powell N.D. Sheridan J.F. Godbout J.P. Peripheral innate immune challenge exaggerated microglia activation, increased the number of inflammatory CNS macrophages, and prolonged social withdrawal in socially defeated mice Psychoneuroendocrinology 37 2012 1491 1505 22386198 PMC3368999
  66. Wohleb E.S. McKim D.B. Sheridan J.F. Godbout J.P. Monocyte trafficking to the brain with stress and inflammation: a novel axis of immune-to-brain communication that influences mood and behavior Front Neurosci. 8 2015 447 25653581 PMC4300916
  67. Yuen E.Y. Jiang Q. Chen P. Feng J. Yan Z. Activation of 5-HT2A/C receptors counteracts 5-HT1A regulation of N-methyl-D-aspartate receptor channels in pyramidal neurons of prefrontal cortex J. Biol. Chem. 283 2008 17194 17204 18442977 PMC2427346

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Authors
Savignac, H. M., Couch, Y., Stratford, M., Bannerman, D. M., Tzortzis, G., Anthony, D. C., & Burnet, P. W. J.
Affiliations
University of Oxford (GB)
Corresponding author
Burnet, Philip W.J.
ORCID
Venue
Brain, Behavior, and Immunity
Publisher
Elsevier BV
Published
2016-02
Volume
52
Pages
120-131
ISSN
0889-1591 (ISSN-L)
DOI
10.1016/j.bbi.2015.10.007
PMC
PMC4927692
OpenAlex
W2137216702
Cited by
223
References
14
Citations by year
2026: 8 · 2025: 11 · 2024: 16 · 2023: 16 · 2022: 37 · 2021: 24 · 2020: 19 · 2019: 27 · 2018: 20 · 2017: 24 · 2016: 21
FWCI
20.88
Type
journal article
Language
en
Open access
Licence
Locations
  • Brain Behavior and Immunity (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust (087736)
  • Biotechnology and Biological Sciences Research Council (BB/I006311/1)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
  • Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
prebiotics, galactooligosaccharides, lipopolysaccharide, sickness behavior, anxiety, IL-1β, 5-HT2A receptor, serotonin, neuroinflammation, gut-brain axis, frontal cortex, neuropsychiatric disorders
MeSH terms
Gastrointestinal Microbiome, Animals, Anxiety, Disease Models, Animal, Frontal Lobe, Hippocampus, Inflammation, Lipopolysaccharides, Male, Serotonin, Receptors, N-Methyl-D-Aspartate, Cytokines, Receptor, Serotonin, 5-HT2A, Mice, Interleukin-1beta, Illness Behavior, Prebiotics

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