Dietary Protein to Carbohydrate Ratio and Caloric Restriction: Comparing Metabolic Outcomes in Mice
Open accessAbstract
Both caloric restriction (CR) and low-protein, high-carbohydrate (LPHC) ad-libitum-fed diets increase lifespan and improve metabolic parameters such as insulin, glucose, and blood lipids. Severe CR, however, is unsustainable for most people; therefore, it is important to determine whether manipulating macronutrient ratios in ad-libitum-fed conditions can generate similar health outcomes. We present the results of a short-term (8 week) dietary manipulation on metabolic outcomes in mice. We compared three diets varying in protein to carbohydrate ratio under both CR and ad libitum conditions. Ad libitum LPHC diets delivered similar benefits to CR in terms of levels of insulin, glucose, lipids, and HOMA, despite increased energy intake. CR on LPHC diets did not provide additional benefits relative to ad libitum LPHC. We show that LPHC diets under ad-libitum-fed conditions generate the metabolic benefits of CR without a 40% reduction in total caloric intake.
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- Authors
- Solon-Biet, S. M., Mitchell, S. J., Coogan, S. C. P., Cogger, V. C., Gokarn, R., McMahon, A. C., Raubenheimer, D., de Cabo, R., Simpson, S. J., & Le Couteur, D. G.
- Affiliations
- National Institutes of Health (US)
- The University of Sydney (AU)
- Corresponding author
- Simpson, Stephen J.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-06
- Volume
- 11
- Issue
- 10
- Pages
- 1529-1534
- ISSN
- 2211-1247 (ISSN-L)
- PMC
- PMC4472496
- OpenAlex
- W2148853147
- Cited by
- 207
- References
- 39
- Citations by year
- 2026: 5 · 2025: 15 · 2024: 17 · 2023: 21 · 2022: 17 · 2021: 24 · 2020: 19 · 2019: 25 · 2018: 28 · 2017: 12 · 2016: 22 · 2015: 2
- FWCI
- 15.72
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health
- National Institute on Aging (Z01AG000363, ZIAAG000368)
- Topics
- Health Sciences › Medicine › Physiology › Dietary Effects on Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
- Keywords
- caloric restriction, protein-carbohydrate ratio, low-protein high-carbohydrate diet, ad libitum feeding, metabolic health, insulin, glucose, serum lipids, HOMA-IR, lifespan, energy intake, macronutrient ratio, mice
- MeSH terms
- Animals, Energy Intake, Dietary Carbohydrates, Dietary Proteins, Glucose, Male, Mice, Inbred C57BL, Random Allocation, Caloric Restriction, Mice
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