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Cited by 316

The DOI matches its Crossref record; 90 of the 90 references it cites were found in a registry or an index.

Single-Cell Sequencing of Developing Human Gut Reveals Transcriptional Links to Childhood Crohn’s Disease

Open access
Elmentaite, Rasa, Ross, Alexander D.B., Roberts, Kenny, James, Kylie R., Ortmann, Daniel, Gomes, Tomás, Nayak, Komal, Tuck, Liz, Pritchard, Sophie, Bayraktar, Omer Ali, Heuschkel, Robert, Vallier, Ludovic, Teichmann, Sarah A., Zilbauer, Matthias·2020·Developmental Cell

Abstract

Human gut development requires the orchestrated interaction of differentiating cell types. Here, we generate an in-depth single-cell map of the developing human intestine at 6-10 weeks post-conception. Our analysis reveals the transcriptional profile of cycling epithelial precursor cells; distinct from LGR5-expressing cells. We propose that these cells may contribute to differentiated cell subsets via the generation of LGR5-expressing stem cells and receive signals from surrounding mesenchymal cells. Furthermore, we draw parallels between the transcriptomes of ex vivo tissues and in vitro fetal organoids, revealing the maturation of organoid cultures in a dish. Lastly, we compare scRNA-seq profiles from pediatric Crohn's disease epithelium alongside matched healthy controls to reveal disease-associated changes in the epithelial composition. Contrasting these with the fetal profiles reveals the re-activation of fetal transcription factors in Crohn's disease. Our study provides a resource available at www.gutcellatlas.org, and underscores the importance of unraveling fetal development in understanding disease.

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Crossref · 10.1016/j.devcel.2020.11.010

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Metadata

Authors
Elmentaite, R., Ross, A. D. B., Roberts, K., James, K. R., Ortmann, D., Gomes, T., Nayak, K., Tuck, L., Pritchard, S., Bayraktar, O. A., Heuschkel, R., Vallier, L., Teichmann, S. A., & Zilbauer, M.
Venue
Developmental Cell
Publisher
Elsevier BV
Published
2020-12
Volume
55
Issue
6
Pages
771-783.e5
ISSN
1534-5807 (ISSN-L)
OpenAlex
W3110852430
Cited by
316
References
90
FWCI
12.03
Type
journal article
Language
en
Open access
hybrid

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