Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis
Open accessAbstract
Objective To evaluate neurotrophin (NT) expression in the endometrium of women with and without endometriosis.Design Prospective, cross-sectional, translational study.Setting Academic hospital.Patient(s) Thirty-three reproductive-age women undergoing laparoscopy for infertility, pelvic pain, intramural fibroids, or tubal ligation.Intervention(s) Endometrial biopsies, protein microarrays, reverse transcriptase-polymerase chain reaction, ELISAs, and Western blotting.Main outcome measure(s) Neurotrophin proteins and mRNAs in eutopic endometrial biopsies.Result(s) Among seven neurotrophic proteins detected on the antibody microarrays, reverse transcriptase-polymerase chain reaction analysis confirmed nerve growth factor, NT-4/5, and brain-derived neurotrophic factor mRNAs in endometrial tissue. Quantitative ELISAs revealed that NT-4/5 (806 ± 701 vs. 256 ± 190 pg/100 mg protein) and brain-derived neurotrophic factor (121 ± 97 vs. 14 ± 11 ng/100 mg protein) concentrations were significantly higher in women with endometriosis. Nerve growth factor (100 ± 74 vs. 93 ± 83 pg/100 mg protein) levels did not differ between cases and controls.Conclusion(s) Neurotrophins are synthesized in situ within the endometrium. NT-4/5 and brain-derived neurotrophic factor proteins were more concentrated in biopsies from endometriosis cases than controls, whereas nerve growth factor levels were similar. We hypothesize that the local production of NTs induces sensory innervation of endometrium of women with endometriosis. These NTs represent novel targets for the diagnosis and treatment of endometriosis.
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Ectopic and eutopic endometrium produce neurotrophic factors, some of which are differentially expressed in cases of endometriosis and could serve as minimally invasive endometriosis biomarkers.
“Not surprisingly, ectopic and eutopic endometrium produce neurotrophic factors, 83 some of which are differentially expressed in cases of endometrioisis 84 and could serve as minimally invasive endometriosis biomarkers.85”
From Defining Future Directions for Endometriosis Research: Workshop Report From the 2011 World Congress of Endometriosis in Montpellier, France (2013) · cites this paper as [84]
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- Authors
- Browne, A. S., Yu, J., Huang, R.-P., Francisco, A. M. C., Sidell, N., & Taylor, R. N.
- Affiliations
- Emory University (US)
- Universidade do Vale do Sapucaí (BR)
- Corresponding author
- Taylor, Robert N.
- Venue
- Fertility and Sterility
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 98
- Issue
- 3
- Pages
- 713-719
- ISSN
- 0015-0282 (ISSN-L)
- PMC
- PMC3432681
- OpenAlex
- W2101625668
- Cited by
- 78
- References
- 40
- Citations by year
- 2026: 4 · 2025: 2 · 2024: 3 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 9 · 2019: 4 · 2018: 9 · 2017: 3 · 2016: 4 · 2015: 11 · 2014: 10 · 2013: 5 · 2012: 1
- FWCI
- 10.05
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Fertility and Sterility (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (KL2TR000455, UL1TR000454, 1r41hd065360-01)
- National Center for Research Resources (UL1RR025008, UL1-RR25009, KL2RR025009, UL1-RR25008)
- National Center for Advancing Translational Sciences
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R41 HD065360, R41-HD65360)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Keywords
- endometriosis, neurotrophins, eutopic endometrium, nerve growth factor, neurotrophin-4/5, brain-derived neurotrophic factor, endometrial biopsy, neurotrophin expression
- MeSH terms
- Adult, Endometriosis, Endometrium, Female, Humans, Middle Aged, Nerve Growth Factors, Blotting, Western, Brain-Derived Neurotrophic Factor, Reverse Transcriptase Polymerase Chain Reaction, Proteomics
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