Cited by 78Endometriosis Research and TreatmentEctopic Pregnancy Diagnosis and ManagementReproductive System and Pregnancy

Proteomic identification of neurotrophins in the eutopic endometrium of women with endometriosis

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Browne, Aimee S., Yu, Jie, Huang, Ruo-Pan, Francisco, Antônio M.C., Sidell, Neil, Taylor, Robert N.·2012·Fertility and Sterility

Abstract

Objective To evaluate neurotrophin (NT) expression in the endometrium of women with and without endometriosis.Design Prospective, cross-sectional, translational study.Setting Academic hospital.Patient(s) Thirty-three reproductive-age women undergoing laparoscopy for infertility, pelvic pain, intramural fibroids, or tubal ligation.Intervention(s) Endometrial biopsies, protein microarrays, reverse transcriptase-polymerase chain reaction, ELISAs, and Western blotting.Main outcome measure(s) Neurotrophin proteins and mRNAs in eutopic endometrial biopsies.Result(s) Among seven neurotrophic proteins detected on the antibody microarrays, reverse transcriptase-polymerase chain reaction analysis confirmed nerve growth factor, NT-4/5, and brain-derived neurotrophic factor mRNAs in endometrial tissue. Quantitative ELISAs revealed that NT-4/5 (806 ± 701 vs. 256 ± 190 pg/100 mg protein) and brain-derived neurotrophic factor (121 ± 97 vs. 14 ± 11 ng/100 mg protein) concentrations were significantly higher in women with endometriosis. Nerve growth factor (100 ± 74 vs. 93 ± 83 pg/100 mg protein) levels did not differ between cases and controls.Conclusion(s) Neurotrophins are synthesized in situ within the endometrium. NT-4/5 and brain-derived neurotrophic factor proteins were more concentrated in biopsies from endometriosis cases than controls, whereas nerve growth factor levels were similar. We hypothesize that the local production of NTs induces sensory innervation of endometrium of women with endometriosis. These NTs represent novel targets for the diagnosis and treatment of endometriosis.

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Crossref · 10.1016/j.fertnstert.2012.05.027

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Authors
Browne, A. S., Yu, J., Huang, R.-P., Francisco, A. M. C., Sidell, N., & Taylor, R. N.
Affiliations
  • Emory University (US)
  • Universidade do Vale do Sapucaí (BR)
Corresponding author
Taylor, Robert N.
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2012-09
Volume
98
Issue
3
Pages
713-719
ISSN
0015-0282 (ISSN-L)
OpenAlex
W2101625668
Cited by
78
References
40
Citations by year
2026: 4 · 2025: 2 · 2024: 3 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 9 · 2019: 4 · 2018: 9 · 2017: 3 · 2016: 4 · 2015: 11 · 2014: 10 · 2013: 5 · 2012: 1
FWCI
10.05
Type
journal article
Language
en
Open access
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (KL2TR000455, UL1TR000454, 1r41hd065360-01)
  • National Center for Research Resources (UL1RR025008, UL1-RR25009, KL2RR025009, UL1-RR25008)
  • National Center for Advancing Translational Sciences
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R41 HD065360, R41-HD65360)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
Keywords
endometriosis, neurotrophins, eutopic endometrium, nerve growth factor, neurotrophin-4/5, brain-derived neurotrophic factor, endometrial biopsy, neurotrophin expression
MeSH terms
Adult, Endometriosis, Endometrium, Female, Humans, Middle Aged, Nerve Growth Factors, Blotting, Western, Brain-Derived Neurotrophic Factor, Reverse Transcriptase Polymerase Chain Reaction, Proteomics

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