Cited by 154Axon Guidance and Neuronal SignalingNerve injury and regenerationProteoglycans and glycosaminoglycans research

Semaphorin 3A displays a punctate distribution on the surface of neuronal cells and interacts with proteoglycans in the extracellular matrix

De Wit, Joris, De Winter, Fred, Klooster, Jan, Verhaagen, Joost·2005·Molecular and Cellular Neuroscience

Abstract

Secreted semaphorins are essential for neural development and continue to be expressed in subpopulations of adult neurons, where they subserve as yet unknown functions. We employed functional myc- and GFP-tagged Sema3A proteins to obtain insight in the localization of Sema3A in neuronal cells. Sema3A localized to both axons and dendrites of cortical neurons. GFP-Sema3A exhibited a characteristic punctate distribution on the surface of Neuro-2a cells, localized to migratory pathways of cultured cells, and co-localized with and induced clustering of its receptor component neuropilin-1. Treatment with excess glycosaminoglycans and chondroitinase ABC resulted in the removal of cell surface Sema3A. Heparin enhanced Sema3A's binding to neuropilin-1-expressing cells and potentiated its growth cone collapsing activity. Together, these results indicate that association with proteoglycans in the extracellular matrix of neuronal cells plays an important role in the localization of the chemorepulsive guidance cue Sema3A, and that this interaction may enhance its biological activity.

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Crossref · 10.1016/j.mcn.2004.12.009

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Authors
De Wit, J., De Winter, F., Klooster, J., & Verhaagen, J.
Corresponding author
Verhaagen, Joost
Venue
Molecular and Cellular Neuroscience
Publisher
Elsevier BV
Published
2005-05
Volume
29
Issue
1
Pages
40-55
ISSN
1044-7431 (ISSN-L)
OpenAlex
W2078936926
Cited by
154
References
92
Citations by year
2026: 5 · 2025: 2 · 2024: 7 · 2023: 7 · 2022: 5 · 2021: 9 · 2020: 6 · 2019: 4 · 2018: 4 · 2017: 4 · 2016: 9 · 2015: 11 · 2014: 9 · 2013: 14 · 2012: 8
FWCI
3.50
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular and Cellular Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • KNAW research portal (Royal Academy of Art and Sciences (KNAW)) (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Lirias (KU Leuven) (submittedVersion)
  • KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
Keywords
semaphorin 3A, neuropilin-1, proteoglycans, extracellular matrix, glycosaminoglycans, chondroitinase ABC, cell surface localization, growth cone collapse, axons and dendrites, cortical neurons, N2a cells, heparin binding
MeSH terms
Animals, Anticoagulants, Epitopes, Cerebral Cortex, Heparin, Membrane Proteins, Neuroblastoma, Neurons, Protein Binding, Proteoglycans, Tumor Cells, Cultured, Proto-Oncogene Proteins c-myc, Extracellular Matrix Proteins, Rats, Wistar, Growth Cones, Neuropilin-1, Semaphorin-3A, Green Fluorescent Proteins, Mice, Rats

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