Shedding of soluble platelet-derived growth factor receptor-β from human brain pericytes
Open accessAbstract
Platelet-derived growth factor receptor-β (PDGFRβ) is expressed in the brain by vascular mural cells-brain capillary pericytes and arterial vascular smooth muscle cells (VSMCs). Recent evidence shows that blood-brain barrier (BBB) disruption and increased permeability, especially in the hippocampus, positively correlates with elevated levels of soluble PDGFRβ (sPDGFRβ) in cerebrospinal fluid (CSF) in patients with mild dementia. To determine which vascular cell type(s) contributes to increased sPDGFRβ in CSF, we compared PDGFRβ expression and sPDGFRβ shedding in response to injury in early passage primary cultures of human brain pericytes, brain arterial VSMCs, and brain endothelial cells. PDGFRβ protein was undetectable in endothelial cells, but was found both in pericytes and VSMCs. PDGFRβ relative protein abundance was by 4.2-fold (p<0.05) higher in pericytes compared to VSMCs. Hypoxia (1% O2) or amyloid-β peptide (25 μM) compared to normoxia (21% O2) both increased over 48 h shedding of sPDGFRβ and its levels in the culture medium from pericytes cultures, but not from VSMCs cultures, by 4.3-fold and 4.6-fold, respectively, compared to the basal sPDGFRβ levels in the medium (1.43±0.15 ng/ml). This was associated with the corresponding loss of cell-associated PDGFRβ from pericytes and no change in cellular levels of PDGFRβ in VSMCs. Thus, sPDGFRβ is a biomarker of pericyte injury, and elevated sPDGFRβ levels in biofluids in patients with dementia and/or other neurodegenerative disorders likely reflects pericyte injury, which supports the potential for sPDGFRβ to be developed and validated as a biomarker of brain pericyte injury and BBB dysfunction.
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PDGFRβ expression is higher in pericytes than in smooth muscle cells.
“PDGFRβ expression in pericytes is noticeably higher than in SMCs18,25,26.”
From Blood–brain barrier breakdown is an early biomarker of human cognitive dysfunction (2019) · cites this paper as [18]
sPDGFRβ is primarily a biomarker of brain capillary pericytes.
“supporting that sPDGFRβ is primarily a biomarker of brain capillary pericytes8,18.”
From Blood–brain barrier breakdown is an early biomarker of human cognitive dysfunction (2019) · cites this paper as [18]
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- Authors
- Sagare, A. P., Sweeney, M. D., Makshanoff, J., & Zlokovic, B. V.
- Affiliations
- University of Southern California (US)
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2015-10
- Volume
- 607
- Pages
- 97-101
- ISSN
- 0304-3940 (ISSN-L)
- PMC
- PMC4631673
- OpenAlex
- W1815758482
- Cited by
- 142
- References
- 37
- Citations by year
- 2026: 14 · 2025: 14 · 2024: 15 · 2023: 17 · 2022: 20 · 2021: 16 · 2020: 15 · 2019: 13 · 2018: 8 · 2017: 4 · 2016: 5 · 2015: 1
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (R01AG023084, R01NS090904, R01NS034467, R01AG039452, 5P50AG005142)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- PDGFRβ, brain pericytes, vascular smooth muscle cells, blood-brain barrier dysfunction, cerebrospinal fluid biomarkers, hypoxia, amyloid-beta, dementia, neurodegenerative diseases, receptor shedding
- MeSH terms
- Brain, Cells, Cultured, Humans, Oxygen, Amyloid beta-Peptides, Pericytes, Receptor, Platelet-Derived Growth Factor beta, Myocytes, Smooth Muscle, Endothelial Cells, Primary Cell Culture
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