Cited by 270Neuroscience and Neuropharmacology ResearchCellular transport and secretionMetabolism, Diabetes, and Cancer

Glycolytic enzymes localize to synapses under energy stress to support synaptic function

Open access
Jang, SoRi, Nelson, Jessica C., Bend, Eric G., Rodríguez-Laureano, Lucelenie, Tueros, Felipe G., Cartagenova, Luis, Underwood, Katherine, Jorgensen, Erik M. +1 more·2016·Neuron

Published version of a preprint

Abstract

Changes in neuronal activity create local and transient changes in energy demands at synapses. Here we discover a metabolic compartment that forms in vivo near synapses to meet local energy demands and support synaptic function in Caenorhabditis elegans neurons. Under conditions of energy stress, glycolytic enzymes redistribute from a diffuse localization in the cytoplasm to a punctate localization adjacent to synapses. Glycolytic enzymes colocalize, suggesting the ad hoc formation of a glycolysis compartment, or a "glycolytic metabolon," that can maintain local levels of ATP. Local formation of the glycolytic metabolon is dependent on presynaptic scaffolding proteins, and disruption of the glycolytic metabolon blocks the synaptic vesicle cycle, impairs synaptic recovery, and affects locomotion. Our studies indicate that under energy stress conditions, energy demands in C. elegans synapses are met locally through the assembly of a glycolytic metabolon to sustain synaptic function and behavior. VIDEO ABSTRACT.

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Crossref · 10.1016/j.neuron.2016.03.011

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  • Substantial direct metabolism of glucose in presynaptic terminals is consistent with an important role for glucose transporters and glycolysis in presynaptic terminals.

    “consistent with an important role for glucose transporters and glycolysis in presynaptic terminals (Jang et al., 2016; Ashrafi et al., 2017; Ashrafi and Ryan, 2017).”

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Authors
Jang, S., Nelson, J. C., Bend, E. G., Rodríguez-Laureano, L., Tueros, F. G., Cartagenova, L., Underwood, K., Jorgensen, E. M., & Colón-Ramos, D. A.
Affiliations
  • Yale University (US)
  • Howard Hughes Medical Institute (US)
  • Universidad Ricardo Palma (PE)
Venue
Neuron
Publisher
Elsevier BV
Published
2016-04
Volume
90
Issue
2
Pages
278-291
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2334665931
Cited by
270
References
89
Citations by year
2026: 23 · 2025: 20 · 2024: 29 · 2023: 24 · 2022: 32 · 2021: 27 · 2020: 23 · 2019: 25 · 2018: 32 · 2017: 23 · 2016: 11
FWCI
17.07
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NIH (P40 OD010440, R01 NS034307)
  • Mitani lab
  • Klingenstein Foundation
  • Alfred P. Sloan Foundation
  • March of Dimes Research
  • NSF (0920069)
  • Cellular, Biochemical, and Molecular Sciences Predoctoral Training Program (T32 GM007223)
  • Universidad Ricardo Palma, Perú
  • Research Experience for Peruvian Undergraduates (REPU) program
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
energy stress, ATP production, synaptic vesicle recycling, synaptic recovery, locomotion, Caenorhabditis elegans neurons
MeSH terms
Animals, Hypoxia, Endocytosis, Mutation, Phosphofructokinase-1, Stress, Physiological, Synaptic Vesicles, Caenorhabditis elegans, Presynaptic Terminals, Metabolomics

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