Cited by 670Mitochondrial Function and PathologyAutophagy in Disease and TherapyCell death mechanisms and regulation

C. elegans Dynamin-Related Protein DRP-1 Controls Severing of the Mitochondrial Outer Membrane

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Labrousse, Arnaud M., Zappaterra, Mauro D., Rube, Daniel A., van der Bliek, Alexander M.·1999·Molecular Cell

Abstract

Little is known about the mechanism of mitochondrial division. We show here that mitochondria are disrupted by mutations in a C. elegans dynamin-related protein (DRP-1). Mutant DRP-1 causes the mitochondrial matrix to retract into large blebs that are both surrounded and connected by tubules of outer membrane. This indicates that scission of the mitochondrial outer membrane is inhibited, while scission of the inner membrane still occurs. Overexpressed wild-type DRP-1 causes mitochondria to become excessively fragmented, consistent with an active role in mitochondrial scission. DRP-1 fused to GFP is observed in spots on mitochondria where scission eventually occurs. These data indicate that wild-type DRP-1 contributes to the final stages of mitochondrial division by controlling scission of the mitochondrial outer membrane.

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Crossref · 10.1016/s1097-2765(00)80391-3

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Authors
Labrousse, A. M., Zappaterra, M. D., Rube, D. A., & van der Bliek, A. M.
Affiliations
University of California, Los Angeles (US)
Venue
Molecular Cell
Publisher
Elsevier BV
Published
1999-11
Volume
4
Issue
5
Pages
815-826
ISSN
1097-2765 (ISSN-L)
OpenAlex
W2126624770
Cited by
670
References
48
Citations by year
2026: 9 · 2025: 21 · 2024: 33 · 2023: 19 · 2022: 20 · 2021: 27 · 2020: 28 · 2019: 24 · 2018: 30 · 2017: 34 · 2016: 19 · 2015: 27 · 2014: 34 · 2013: 25 · 2012: 32
FWCI
5.74
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (R01GM051866, GM 51866)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Keywords
Drp1, dynamin-related proteins, mitochondrial division, mitochondrial fission, mitochondrial fragmentation, Caenorhabditis elegans, mitochondrial matrix
MeSH terms
Animals, Cell Division, Female, Genes, Lethal, Humans, Intracellular Membranes, Membrane Proteins, Mitochondria, Molecular Sequence Data, Muscles, Mutation, Neuropeptides, Oogenesis, Recombinant Fusion Proteins, RNA, Messenger, Tetradecanoylphorbol Acetate, Gene Expression, Consensus Sequence, Caenorhabditis elegans, Sequence Homology, Amino Acid, Cell Lineage, Gene Expression Profiling, Drosophila Proteins, Caenorhabditis elegans Proteins, Vesicular Transport Proteins, Dynamins

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