Transgenic stem cells in Hydra reveal an early evolutionary origin for key elements controlling self-renewal and differentiation
Open accessAbstract
Little is known about stem cells in organisms at the beginning of evolution. To characterize the regulatory events that control stem cells in the basal metazoan Hydra, we have generated transgenics which express eGFP selectively in the interstitial stem cell lineage. Using them we visualized stem cell and precursor migration in real-time in the context of the native environment. We demonstrate that interstitial cells respond to signals from the cellular environment, and that Wnt and Notch pathways are key players in this process. Furthermore, by analyzing polyps which overexpress the Polycomb protein HyEED in their interstitial cells, we provide in vivo evidence for a role of chromatin modification in terminal differentiation. These findings for the first time uncover insights into signalling pathways involved in stem cell differentiation in the Bilaterian ancestor; they demonstrate that mechanisms controlling stem cell behaviour are based on components which are conserved throughout the animal kingdom.
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Self-citation
- Authors
- Khalturin, K., Anton-Erxleben, F., Milde, S., Plötz, C., Wittlieb, J., Hemmrich, G., & Bosch, T. C. G.
- Affiliations
- Christian-Albrechts-Universität zu Kiel (DE)
- Corresponding author
- Bosch, Thomas C.G.
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2007-09
- Volume
- 309
- Issue
- 1
- Pages
- 32-44
- ISSN
- 0012-1606 (ISSN-L)
- OpenAlex
- W2157147630
- Cited by
- 125
- References
- 70
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 3 · 2020: 3 · 2019: 8 · 2018: 6 · 2017: 6 · 2016: 6 · 2015: 6 · 2014: 7 · 2013: 6 · 2012: 16
- FWCI
- 9.64
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2182 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Keywords
- Hydra, interstitial stem cells, stem cell self-renewal, stem cell differentiation, Wnt signaling, Notch signaling, Polycomb group proteins, chromatin modification, terminal differentiation, stem cell migration, transgenic reporter, basal metazoans
- MeSH terms
- Animals, Cell Differentiation, Biological Evolution, Hydra, Stem Cells, Signal Transduction, Animals, Genetically Modified, Extracellular Fluid, Wnt Proteins, Receptors, Notch
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