Cited by 307Algal biology and biofuel productionMicrobial Metabolic Engineering and BioproductionPlant biochemistry and biosynthesis

Trophic Conversion of an Obligate Photoautotrophic Organism Through Metabolic Engineering

Zaslavskaia, L. A., Lippmeier, J. C., Shih, C., Ehrhardt, D., Grossman, A. R., Apt, K. E.·2001·Science

Abstract

Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically derived energy. We show that the microalga Phaeodactylum tricornutum can be genetically engineered to thrive on exogenous glucose in the absence of light through the introduction of a gene encoding a glucose transporter ( glut1 or hup1 ). This demonstrates that a fundamental change in the metabolism of an organism can be accomplished through the introduction of a single gene. This also represents progress toward the use of fermentation technology for large-scale commercial exploitation of algae by reducing limitations associated with light-dependent growth.

Integrity signals

Registry

Crossref · 10.1126/science.160015

Cites

25 of 25 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Algae production strains have the potential to be bioengineered, allowing improvement of specific traits and production of valuable co-products, which may allow algal biofuels to compete economically with petroleum.

    “Algae production strains also have the potential to be bioengineered, allowing improvement of specific traits [25,26] and production of valuable co-products, which may allow algal biofuels to compete economically with petroleum.”

    From Biofuels from algae: Challenges and potential (2010) · cites this paper as [26]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Zaslavskaia, L. A., Lippmeier, J. C., Shih, C., Ehrhardt, D., Grossman, A. R., & Apt, K. E.
Affiliations
  • Martek Biosciences Corp., 6480 Dobbin Road, Columbia, MD 21045, USA.
  • Carnegie Institution for Science (US)
Corresponding author
Apt, K. E.
Venue
Science
Published
2001-06-15
Volume
292
Issue
5524
Pages
2073-2075
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1973319807
Cited by
307
References
25
Citations by year
2026: 4 · 2025: 8 · 2024: 10 · 2023: 9 · 2022: 18 · 2021: 17 · 2020: 8 · 2019: 16 · 2018: 8 · 2017: 14 · 2016: 17 · 2015: 15 · 2014: 11 · 2013: 17 · 2012: 12
FWCI
15.47
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
Phaeodactylum tricornutum, microalgae, glucose utilization, glucose transporters, GLUT1, photoautotrophy, heterotrophic growth, metabolic engineering, fermentation
MeSH terms
Biological Transport, Cell Line, Transformed, Cell Membrane, Darkness, Genetic Engineering, Glucose, Glycolysis, Humans, Light, Luminescent Proteins, Membrane Proteins, Molecular Weight, Monosaccharide Transport Proteins, Photosynthesis, Recombinant Fusion Proteins, Transformation, Genetic, Diatoms, Microscopy, Confocal, Symporters, Green Fluorescent Proteins, Glucose Transporter Type 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review