Disturbance of perineuronal nets in the perilesional area after photothrombosis is not associated with neuronal death
Abstract
Perineuronal nets (PNNs) are a condensed form of extracellular matrix that covers the surface of a subset of neurons. Their presence limits neuronal plasticity and may protect neurons against harmful agents. Here we analyzed the relationship between spatiotemporal changes in PNN expression and cell death markers after focal cortical photothrombotic stroke in rats. We registered a substantial decrease in PNN density using Wisteria floribunda agglutinin staining and CAT-315 and brevican immunoreactivity; the decrease occurred not only in the lesion core but also in the perilesional and remote cortex as well as in homotopic contralateral cortical regions. Fluoro Jade C and TUNEL staining in perilesional and remote areas, however, showed a low density of dying cells. Our results suggest that the PNN reduction was not a result of cellular death and could be considered an attempt to create conditions favorable for synaptic remodeling.
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Data on changes in PNs after stroke remain rather limited.
“However, data on the changes in PNs after stroke remain rather limited.”
From Damaged Neocortical Perineuronal Nets Due to Experimental Focal Cerebral Ischemia in Mice, Rats and Sheep (2017) · cites this paper as Karetko-Sysa et al., 2011
Abolished and decomposed PNs were reported for other animal models of experimentally induced stroke in neocortices of rats.
“Based on WFA staining, abolished and decomposed PNs were also reported for other animal models of experimentally induced stroke in neocortices of rats (Hobohm et al., 2005; Karetko-Sysa et al., 2011; Madinier et al., 2014; Quattromani et al., 2017).”
From Damaged Neocortical Perineuronal Nets Due to Experimental Focal Cerebral Ischemia in Mice, Rats and Sheep (2017) · cites this paper as Karetko-Sysa et al., 2011
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A study of neurodegeneration and neuroprotection in Nothobranchius guentheri
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- Authors
- Karetko-Sysa, M., Skangiel-Kramska, J., & Nowicka, D.
- Affiliations
- Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
- Corresponding author
- Nowicka, Dorota
- ORCID
- Nowicka, Dorota
- Venue
- Experimental Neurology
- Publisher
- Elsevier BV
- Published
- 2011-09
- Volume
- 231
- Issue
- 1
- Pages
- 113-126
- ISSN
- 0014-4886 (ISSN-L)
- OpenAlex
- W2045240514
- Cited by
- 45
- References
- 91
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 5 · 2019: 1 · 2018: 3 · 2017: 9 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 5 · 2012: 2
- FWCI
- 1.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Neurology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Keywords
- perineuronal nets, photothrombotic stroke, perilesional cortex, neuronal cell death, extracellular matrix, brevican, Wisteria floribunda agglutinin, synaptic remodeling, neuroplasticity
- MeSH terms
- Animals, Cerebral Cortex, Disease Models, Animal, Extracellular Matrix, Male, Nerve Degeneration, Nerve Net, Neurons, Cell Death, Rats, Wistar, Brain Infarction, Intracranial Thrombosis, Rats
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