Differential expression of mRNA for leptin receptor isoforms in the rat brain

Guan, Xiao-Ming; Hess, J.Fred; Yu, Hong; Hey, Patricia J; van der Ploeg, Lex H.T

doi:10.1016/s0303-7207(97)00138-x

Cited by 173Regulation of Appetite and ObesityAdipokines, Inflammation, and Metabolic DiseasesHypothalamic control of reproductive hormones

Differential expression of mRNA for leptin receptor isoforms in the rat brain

Guan, Xiao-Ming, Hess, J.Fred, Yu, Hong, Hey, Patricia J, van der Ploeg, Lex H.T·1997·Molecular and Cellular Endocrinology

DOI 10.1016/s0303-7207(97)00138-x

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Abstract

Leptin plays an important role in the control of food intake and energy metabolism by interacting with its receptor (OB-R) in the brain. Several alternatively spliced isoforms of OB-R have been identified. To study the expression patterns and the potential biological function of these OB-Rs in the brain, the distribution of mRNA encoding OB-R isoforms was examined by in situ hybridization. In agreement with previous studies, strong signals for OB-R mRNA were detected in the hypothalamus, thalamus and choroid plexus. In addition, intense signals were observed in several other brain areas including piriform cortex, granule cell layer of the cerebellum and substantia nigra. With isoform-specific probes, a differential expression pattern of OB-Rs was revealed: OB-Ra and OB-Rb, but not OB-Rc and OB-Rf, are abundantly expressed in the hypothalamus, whereas OB-Ra, OB-Rc and OB-Rf, but not OB-Rb, are significantly expressed in the choroid plexus. The preferential expression of OB-Rb in the hypothalamus is in support of its role in mediating the satiety effect of leptin. The co-expression of OB-Ra with OB-Rb in the hypothalamus may suggest a possible interaction between the two isoforms. Finally, the detection of OB-R mRNA in a number of other brain regions may indicate the involvement of leptin in additional as yet undefined physiological functions.

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Leptin may modulate sensory processing at higher gustatory and olfactory processing steps, as indicated by the presence of leptin receptors and leptin-induced Fos expression in the nucleus of the solitary tract, parabrachial nucleus, olfactory bulb, and insular and piriform cortices of rodents.
“Leptin may also modulate sensory processing at higher gustatory and olfactory processing steps, as indicated by the presence of leptin receptors and leptin-induced Fos expression in the nucleus of the solitary tract, parabrachial nucleus, olfactory bulb, and insular and piriform cortices of rodents”
From Food reward, hyperphagia, and obesity (2011) · cites this paper as [74]

Positional cloning of the mouse obese gene and its human homologue
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., & Friedman, J. M. (1994) · Nature · doi:10.1038/372425a0 · Cited by 13,419 · see citing works
Principles of neural science
(1982) · Trends in Neurosciences · doi:10.1016/0166-2236(82)90076-5 · Cited by 9,148 · see citing works
Effects of the obese Gene Product on Body Weight Regulation in ob / ob Mice
Pelleymounter, M. A., Cullen, M. J., Baker, M. B., Hecht, R., Winters, D., Boone, T., & Collins, F. (1995) · Science · doi:10.1126/science.7624776 · Cited by 4,017 · see citing works
Identification and expression cloning of a leptin receptor, OB-R.
Tartaglia, L. A., Dembski, M., Weng, X., Deng, N., Culpepper, J., Devos, R., Richards, G. J., Campfield, L. A., Clark, F. T., Deeds, J., et al. (1995) · Cell · doi:10.1016/0092-8674(95)90151-5 · Cited by 3,496 · see citing works
Role of leptin in the neuroendocrine response to fasting
Ahima, R. S., Prabakaran, D., Mantzoros, C., Qu, D., Lowell, B., Maratos-Flier, E., & Flier, J. S. (1996) · Nature · doi:10.1038/382250a0 · Cited by 3,117 · see citing works
Abnormal splicing of the leptin receptor in diabetic mice
Lee, G.-H., Proenca, R., Montez, J. M., Carroll, K. M., Darvishzadeh, J. G., Lee, J. I., & Friedman, J. M. (1996) · Nature · doi:10.1038/379632a0 · Cited by 2,327 · see citing works
Evidence That the Diabetes Gene Encodes the Leptin Receptor: Identification of a Mutation in the Leptin Receptor Gene in db/db Mice
Chen, H., Charlat, O., Tartaglia, L. A., Woolf, E. A., Weng, X., Ellis, S. J., Lakey, N. D., Culpepper, J., More, K. J., Breitbart, R. E., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81294-5 · Cited by 2,214 · see citing works
Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin
Chehab, F. F., Lim, M. E., & Lu, R. (1996) · Nature Genetics · doi:10.1038/ng0396-318 · Cited by 1,476 · see citing works
Leptin enters the brain by a saturable system independent of insulin
Banks, W. A., Kastin, A. J., Huang, W., Jaspan, J. B., & Maness, L. M. (1996) · Peptides · doi:10.1016/0196-9781(96)00025-3 · Cited by 1,222 · see citing works
Localization of leptin receptor mRNA and the long form splice variant (Ob‐Rb) in mouse hypothalamus and adjacent brain regions by in situ hybridization
Mercer, J. G., Hoggard, N., Williams, L. M., Lawrence, C. B., Hannah, L. T., & Trayhurn, P. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00473-5 · Cited by 880 · see citing works
Leptin receptor missense mutation in the fatty Zucker rat
Phillips, M. S., Liu, Q., Hammond, H. A., Dugan, V., Hey, P. J., Caskey, C. T., & Hess, J. F. (1996) · Nature Genetics · doi:10.1038/ng0596-18 · Cited by 822 · see citing works
Self-citation
The full-length leptin receptor has signaling capabilities of interleukin 6-type cytokine receptors.
Baumann, H., Morella, K. K., White, D. W., Dembski, M., Bailon, P., Kim, H., Lai, C. F., & Tartaglia, L. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.16.8374 · Cited by 810 · see citing works
Defective STAT signaling by the leptin receptor in diabetic mice.
Ghilardi, N., Ziegler, S., Wiestner, A. C., Stoffel, R., Heim, M. H., & Skoda, R. C. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.13.6231 · Cited by 793 · see citing works
Novel B219/OB receptor isoforms: Possible role of leptin in hematopoiesis and reproduction
Cioffi, J. A., Shafer, A., Zupancic, T. J., Smith-Gbur, J., Mikhail, A. A., Platika, D., & Snodgrass, H. R. (1996) · Nature Medicine · doi:10.1038/nm0596-585 · Cited by 616 · see citing works
Evidence of free and bound leptin in human circulation. Studies in lean and obese subjects and during short-term fasting.
Sinha, M. K., Opentanova, I., Ohannesian, J. P., Kolaczynski, J. W., Heiman, M. L., Hale, J. E., Becker, G. W., Bowsher, R. R., Stephens, T. W., & Caro, J. F. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118913 · Cited by 531 · see citing works
A crucial epileptogenic site in the deep prepiriform cortex
Piredda, S., & Gale, K. (1985) · Nature · doi:10.1038/317623a0 · Cited by 433 · see citing works
Leptin Receptor (OB-R) Signaling
White, D. W., Kuropatwinski, K. K., Devos, R., Baumann, H., & Tartaglia, L. A. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.7.4065 · Cited by 293 · see citing works
Olfactory cortex: model circuit for study of associative memory?
Haberly, L. B., & Bower, J. M. (1989) · Trends in Neurosciences · doi:10.1016/0166-2236(89)90025-8 · Cited by 292 · see citing works
A novel leptin receptor isoform in rat
Wang, M.-Y., Yan, T. Z., Newgard, C. B., & Unger, R. H. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00790-9 · Cited by 202 · see citing works
Functional STAT 1 and 3 signaling by the leptin receptor (OB-R); reduced expression of the rat fatty leptin receptor in transfected cells.
Rosenblum, C. I., Tota, M. R., Cully, D. F., et al. (1996) · Endocrinology · doi:10.1210/endo.137.11.8895396 · Cited by 120 · see citing works
OB protein binds specifically to the choroid plexus of mice and rats.
Devos, R., Richards, J. G., Campfield, L. A., Tartaglia, L. A., Guisez, Y., der Heyden, J. V., Travernier, J., Plaetinck, G., & Burn, P. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.11.5668 · Cited by 118 · see citing works
Localization of binding sites in the central nervous system for leptin (OB protein) in normal, obese (ob/ob), and diabetic (db/db) C57BL/6J mice.
Malik, K. F., & Young, W. S. (1996) · Endocrinology · doi:10.1210/endo.137.4.8625929 · Cited by 98 · see citing works
Localization of binding sites in the central nervous system for leptin (OB protein) in normal, obese (ob/ob), and diabetic (db/db) C57BL/6J mice
Malik, K. F. (1996) · Endocrinology · doi:10.1210/en.137.4.1497 · Cited by 32 · see citing works
Neuroanatomical regions of the chick brain involved in monitoring amino acid deficient diets
Firman, J. D., & Kuenzel, W. J. (1988) · Brain Research Bulletin · doi:10.1016/0361-9230(88)90203-1 · Cited by 26 · see citing works
Imaging gene expression in neural grafts
Sirinathsinghji, D. J. S., & Dunnett, S. B. (1994) · doi:10.1093/oso/9780199633814.003.0003 · Cited by 12 · see citing works
Preparation of Functionally Active [35S]Leptin for Mapping Its Receptors in the Brain
Guan, X.-M., Yu, H., der Ploeg, L. H. T. V., et al. (1997) · Analytical Biochemistry · doi:10.1006/abio.1997.2029 · Cited by 3 · see citing works
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Elmquist, J. K., Elias, C. F., & Saper, C. B. (1999) · Neuron · doi:10.1016/s0896-6273(00)81084-3 · Cited by 1,160 · see citing works
The Meter of Metabolism
Green, C. B., Takahashi, J. S., & Bass, J. (2008) · Cell · doi:10.1016/j.cell.2008.08.022 · Cited by 902 · see citing works
Metabolism and Circadian Rhythms—Implications for Obesity
Froy, O. (2010) · Endocrine Reviews · doi:10.1210/er.2009-0014 · Cited by 505 · see citing works
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Grill, H. J., Schwartz, M. W., Kaplan, J. M., Foxhall, J. S., Breininger, J., & Baskin, D. G. (2002) · Endocrinology · doi:10.1210/endo.143.1.8589 · Cited by 395 · see citing works
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Berthoud, H.-R., Lenard, N. R., & Shin, A. C. (2011) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00028.2011 · Cited by 275 · see citing works
Circadian clocks: setting time by food
Mendoza, J. (2007) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2006.01510.x · Cited by 257 · see citing works
Leptin Receptor Long-form Splice-variant Protein Expression in Neuron Cell Bodies of the Brain and Co-localization with Neuropeptide Y mRNA in the Arcuate Nucleus
Baskin, D. G., Schwartz, M. W., Seeley, R. J., Woods, S. C., Porte, D., Breininger, J. F., Jonak, Z., Schaefer, J., Krouse, M., Burghardt, C., et al. (1999) · Journal of Histochemistry & Cytochemistry · doi:10.1177/002215549904700309 · Cited by 200 · see citing works
Circadian Rhythms, Aging, and Life Span in Mammals
Froy, O. (2011) · Physiology · doi:10.1152/physiol.00012.2011 · Cited by 156 · see citing works
Obesity, leptin, and Alzheimer's disease
Lee, E. B. (2011) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2011.06274.x · Cited by 126 · see citing works
Conditional viral tracing reveals that steroidogenic factor 1-positive neurons of the dorsomedial subdivision of the ventromedial hypothalamus project to autonomic centers of the hypothalamus and hindbrain
Lindberg, D., Chen, P., & Li, C. (2013) · Journal of Comparative Neurology · doi:10.1002/cne.23338 · Cited by 125 · see citing works
Suppression of Leptin Receptor Messenger Ribonucleic Acid and Leptin Responsiveness in the Ventromedial Nucleus of the Hypothalamus during Pregnancy in the Rat
Ladyman, S. R., & Grattan, D. R. (2005) · Endocrinology · doi:10.1210/en.2005-0194 · Cited by 115 · see citing works
Role of brain transmigrating neutrophils in depression-like behavior during systemic infection
Aguliar-Valles, A., Kim, J., Jung, S., Woodside, B., & Luheshi, G. N. (2014) · Molecular Psychiatry · doi:10.1038/mp.2013.137 · Cited by 97 · see citing works
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Evans, M. C., & Anderson, G. M. (2018) · Endocrinology · doi:10.1210/en.2018-00691 · Cited by 41 · see citing works

Localization of 5-oxo-l-prolinase mRNA in the murine choroid plexus by in situ hybridization
Jäger, M., & Wolf, S. (1999) · Neuroscience Letters · doi:10.1016/s0304-3940(99)00718-1
Normal Leptin Expression, Lower Adipogenic Ability, Decreased Leptin Receptor and Hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis
Liang, G., Gao, W., Liang, A., Ye, W., Peng, Y., Zhang, L., Sharma, S., Su, P., & Huang, D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0036648 · Cited by 34 · see citing works
Synthesis of transthyretin (pre-albumin) mRNA in choroid plexus epithelial cells, localized by in situ hybridization in rat brain.
Stauder, A., Dickson, P. W., Aldred, A. R., et al. (1986) · Journal of Histochemistry & Cytochemistry · doi:10.1177/34.7.3458812 · Cited by 83 · see citing works
Leptin and leptin receptor: Analysis of a structure to function relationship in interaction and evolution from humans to fish
Prokop, J. W., Duff, R. J., Ball, H. C., Copeland, D. L., & Londraville, R. L. (2012) · Peptides · doi:10.1016/j.peptides.2012.10.002 · Cited by 93 · see citing works
Phenotypic Consequences of a Nonsense Mutation in the Leptin Receptor Gene (fak) in Obese Spontaneously Hypertensive Koletsky Rats (SHROB)
Ishizuka, T., Ernsberger, P. R., Liu, S., Bedol, D., Lehman, T. M., Koletsky, R. J., & Friedman, J. E. (1998) · Journal of Nutrition · doi:10.1093/jn/128.12.2299 · Cited by 58 · see citing works

Authors: Guan, X.-M., Hess, J. F., Yu, H., Hey, P. J., & van der Ploeg, L. H. T.
Affiliations: Merck & Co., Inc., Rahway, NJ, USA (United States) (US)
Corresponding author: Guan, Xiao-Ming
ORCID: Guan, Xiao-Ming
Yu, Hong
Venue: Molecular and Cellular Endocrinology
Publisher: Elsevier BV
Published: 1997-09
Volume: 133
Issue: 1
Pages: 1-7
ISSN: 0303-7207 (ISSN-L)
DOI: 10.1016/s0303-7207(97)00138-x
OpenAlex: W1992485264
Cited by: 173
References: 26
Citations by year: 2025: 1 · 2024: 2 · 2023: 5 · 2022: 2 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 2 · 2017: 8 · 2016: 6 · 2015: 4 · 2014: 7 · 2013: 7 · 2012: 7
FWCI: 2.89
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Molecular and Cellular Endocrinology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
Keywords: leptin receptor, alternative splicing, hypothalamus, choroid plexus, Ob-Ra, Ob-Rb, satiety, food intake regulation, energy metabolism, brain mRNA expression, thalamus, substantia nigra, piriform cortex, cerebellum
MeSH terms: Amino Acid Sequence, Animals, Brain, Carrier Proteins, Cloning, Molecular, Hypothalamus, Isomerism, Male, Molecular Sequence Data, Obesity, Receptors, Cell Surface, RNA, Messenger, Rats, Sprague-Dawley, Alternative Splicing, Receptors, Cytokine, Rats, Receptors, Leptin

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Differential expression of mRNA for leptin receptor isoforms in the rat brain

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