Micro Total Bioassay System for Ingested Substances: Assessment of Intestinal Absorption, Hepatic Metabolism, and Bioactivity
Abstract
Oral medicines and food constituents are absorbed in the intestine and metabolized in the liver, after which they exhibit their activity toward a target tissue. Micromodels of human tissues were developed to mimic these processes and bioactivities. By integrating the micromodels, we realized a micro total bioassay system for oral substances; this system comprised a microintestine, microliver, and the target components. The microchip was composed of a slide glass and polydimethylsiloxane (PDMS) sheets with microchannels fabricated by photolithography. Caco-2 cells were cultured in the intestine component, and HepG2 cells, in the liver component. The human breast carcinoma MCF-7 cells were cultured in the target component, and the activities of anticancer agents and estrogen-like substances were successfully assayed. By using this system, the overall properties of the ingested cyclophosphamide, epirubicin, 17-β estradiol, and soy isoflavone, i.e., their intestinal absorption, hepatic metabolism, and bioactivity toward target cells, could be assayed with operative ease. Further, the assay time and cell consumption were reduced compared to those in conventional in vitro bioassay systems.
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In a breast cancer/liver microenvironment chip, breast cancer cells cultured in the tumor microenvironment have higher vascular porosity and tumor microenvironment permeability than blood vessels in healthy liver microenvironment.
“Compared with blood vessels in the healthy liver microenvironment, breast cancer cells cultured in the microenvironment of this model have higher vascular porosity and tumor microenvironment permeability.”
From Tumor-on-a-chip: from bioinspired design to biomedical application (2021) · cites this paper as [220]
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- Authors
- Imura, Y., Sato, K., & Yoshimura, E.
- Affiliations
- The University of Tokyo (JP)
- Corresponding author
- Sato, Kiichi
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-11-23
- Volume
- 82
- Issue
- 24
- Pages
- 9983-9988
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2089859139
- Cited by
- 172
- References
- 30
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 10 · 2023: 8 · 2022: 7 · 2021: 11 · 2020: 21 · 2019: 16 · 2018: 9 · 2017: 19 · 2016: 12 · 2015: 10 · 2014: 13 · 2013: 10 · 2012: 10
- FWCI
- 5.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- RIKEN
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Innovative Microfluidic and Catalytic Techniques Innovation
- Keywords
- intestinal absorption, hepatic metabolism, oral drug absorption, Caco-2 cells, HepG2 cells, MCF-7 cells, anticancer agents, cyclophosphamide, epirubicin, estradiol, soy isoflavones, microfluidics, PDMS microchannel, in vitro bioassay
- MeSH terms
- Antineoplastic Agents, Cyclophosphamide, Estradiol, Humans, Intestinal Absorption, Isoflavones, Liver, Models, Biological, Epirubicin, Cell Line, Tumor, Microchip Analytical Procedures, Lab-On-A-Chip Devices
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