Cyanine-Based Polymer Dots with Long-Wavelength Excitation and Near-Infrared Fluorescence beyond 900 nm for In Vivo Biological Imaging
Abstract
with deep penetration, high spatiotemporal resolution, and minimal tissue absorption, scattering, and autofluorescence. In spite of the huge progress on the synthesis of small organic fluorophores and inorganic nanomaterials with emissions beyond 900 nm, it remains a tough challenge to synthesize semiconducting polymers with fluorescence over this region. Here, we synthesized a series of heptamethine cyanine-based polymers with both absorption and emission in the near-infrared region. We prepared these polymers as semiconducting polymer dots (Pdots) in pure water with great biocompatibility. The fluorescence quantum yield of the Pdots can be as high as 14% with a full width at half-maximum of 53 nm, and their single-particle brightness is more than 20 times higher than commercial quantum dots or ∼300 times brighter than Food and Drug Administration (FDA)-approved indocyanine green (ICG) dyes. We further demonstrated the use of cyanine-based Pdots for specific cellular labeling and long-term tumor targeting in mice. We anticipate that these cyanine-based ultrabright Pdots could open up an avenue for next generations of near-infrared fluorescent agents.
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SWIR emitting Pdots consisting of conjugated polymers bearing fluorescent dyes have been developed by incorporating a π-bridge selenyl benzothiadiazole, SWIR emitting polymethine dyes NIR1125, NIR1270, or NIR1380 as an energy acceptor, and a bulky anti-ACQ unit pentiptycene into one CP backbone.
“SWIR emitting Pdots that consist of CPs bearing fluorescent dyes have been developed by incorporating a π-bridge selenyl benzothiadiazole (SeBTa), SWIR emitting polymethine dyes, NIR1125, NIR1270, or NIR1380, as an energy acceptor, and a bulky anti-ACQ unit, pentiptycene (Pttc) into one CP backbone (Figure 13b).194”
From The Pursuit of Shortwave Infrared-Emitting Nanoparticles with Bright Fluorescence through Molecular Design and Excited-State Engineering of Molecular Aggregates (2022) · cites this paper as [194]
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- Authors
- Liu, M.-H., Chen, T.-C., Vicente, J. R., Yao, C.-N., Yang, Y.-C., Chen, C.-P., Lin, P.-W., Ho, Y.-C., Chen, J., Lin, S.-Y., & Chan, Y.-H.
- Affiliations
- National Yang Ming Chiao Tung University (TW)
- Ohio University (US)
- National Health Research Institutes (TW)
- Corresponding author
- Chan, Yang-Hsiang
- ORCID
- Venue
- ACS Applied Bio Materials
- Publisher
- American Chemical Society (ACS)
- Published
- 2020-05-25
- Volume
- 3
- Issue
- 6
- Pages
- 3846-3858
- ISSN
- 2576-6422 (ISSN-L)
- OpenAlex
- W3030188100
- Cited by
- 13
- References
- 68
- Citations by year
- 2026: 1 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1
- FWCI
- 0.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Applied Bio Materials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education
- Ministry of Science and Technology, Taiwan (105-2113-M-110-012-MY3)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Luminescence and Fluorescent Materials
- Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- polymer dots, heptamethine cyanine, near-infrared fluorescence, semiconducting polymers, biocompatibility, fluorescence quantum yield, cell labeling, tumor targeting, in vivo imaging
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