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Phylogenetic distribution of three pathways for propionate production within the human gut microbiota

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Reichardt, Nicole, Duncan, Sylvia H, Young, Pauline, Belenguer, Alvaro, McWilliam Leitch, Carol, Scott, Karen P, Flint, Harry J, Louis, Petra·2014·The ISME Journal
DOI 10.1038/ismej.2014.14
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Abstract

Propionate is produced in the human large intestine by microbial fermentation and may help maintain human health. We have examined the distribution of three different pathways used by bacteria for propionate formation using genomic and metagenomic analysis of the human gut microbiota and by designing degenerate primer sets for the detection of diagnostic genes for these pathways. Degenerate primers for the acrylate pathway (detecting the lcdA gene, encoding lactoyl-CoA dehydratase) together with metagenomic mining revealed that this pathway is restricted to only a few human colonic species within the Lachnospiraceae and Negativicutes. The operation of this pathway for lactate utilisation in Coprococcus catus (Lachnospiraceae) was confirmed using stable isotope labelling. The propanediol pathway that processes deoxy sugars such as fucose and rhamnose was more abundant within the Lachnospiraceae (based on the pduP gene, which encodes propionaldehyde dehydrogenase), occurring in relatives of Ruminococcus obeum and in Roseburia inulinivorans. The dominant source of propionate from hexose sugars, however, was concluded to be the succinate pathway, as indicated by the widespread distribution of the mmdA gene that encodes methylmalonyl-CoA decarboxylase in the Bacteroidetes and in many Negativicutes. In general, the capacity to produce propionate or butyrate from hexose sugars resided in different species, although two species of Lachnospiraceae (C. catus and R. inulinivorans) are now known to be able to switch from butyrate to propionate production on different substrates. A better understanding of the microbial ecology of short-chain fatty acid formation may allow modulation of propionate formation by the human gut microbiota.

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Metadata

Authors
Reichardt, N., Duncan, S. H., Young, P., Belenguer, A., McWilliam Leitch, C., Scott, K. P., Flint, H. J., & Louis, P.
Venue
The ISME Journal
Publisher
Oxford University Press (OUP)
Published
2014-02-20
Volume
8
Issue
6
Pages
1323-1335
ISSN
  • 1751-7362 (ISSN-L)
  • 1751-7370
DOI
10.1038/ismej.2014.14
OpenAlex
W1990590570
Cited by
1,289
References
11
FWCI
16.79
Type
journal article
Language
en
Update notices
erratum (2014-05-20)
Open access
bronze
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CoChat. (2026). Citation check: Phylogenetic distribution of three pathways for propionate production within the human gut microbiota. https://app.cochat.ai/papers/10.1038/ismej.2014.14
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Reichardt, N., Duncan, S. H., Young, P., Belenguer, A., McWilliam Leitch, C., Scott, K. P., Flint, H. J., & Louis, P. (2014). Phylogenetic distribution of three pathways for propionate production within the human gut microbiota. The ISME Journal. https://doi.org/10.1038/ismej.2014.14

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