The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Efficient genome editing in zebrafish using a CRISPR-Cas system
Open accessAbstract
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
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“Also in January 2013, similar publications came out from the laboratories of Jennifer Doudna (Berkeley College, USA) [36], Jin-Soo Kim (Seoul University, South Korea) [37] and J. Keith Joung (Harvard School of Medicine, USA) [38].”
From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [38]
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“Recently, the CRISPR/Cas system has been successfully applied to human cells19,20,21,22 and several model organisms for genome editing.23,24,25,26”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [24]
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Citations
Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
CRISPR/Cas, the Immune System of Bacteria and Archaea
Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471
Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes
Brouns, S. J. J., Jore, M. M., Lundgren, M., Westra, E. R., Slijkhuis, R. J. H., Snijders, A. P. L., Dickman, M. J., Makarova, K. S., Koonin, E. V., & van der Oost, J. (2008) · Science · doi:10.1126/science.1159689 · Cited by 2,460
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
RNA-guided genetic silencing systems in bacteria and archaea
Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012) · Nature · doi:10.1038/nature10886 · Cited by 1,851
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Li, J., Wu, P., Cao, Z., Huang, G., Lu, Z., Yan, J., Zhang, H., Zhou, Y., Liu, R., Chen, H., et al. (2024) · Cell Reports · doi:10.1016/j.celrep.2024.113765 · Cited by 26
CRISP-view: a database of functional genetic screens spanning multiple phenotypes
Cui, Y., Cheng, X., Chen, Q., Song, B., Chiu, A., Gao, Y., Dawson, T., Chao, L., Zhang, W., Li, D., et al. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkaa809 · Cited by 24
VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1
Ugur, B., Schueder, F., Shin, J., Hanna, M. G., Wu, Y., Leonzino, M., Su, M., McAdow, A. R., Wilson, C., Postlethwait, J., et al. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202311189 · Cited by 18
Grandchamp, N., Altémir, D., Philippe, S., Ursulet, S., Pilet, H., Serre, M.-C., Lenain, A., Serguera, C., Mallet, J., & Sarkis, C. (2014) · PLoS ONE · doi:10.1371/journal.pone.0099649 · Cited by 11
Solanki, A. K., Kondkar, A. A., Fogerty, J., Su, Y., Kim, S.-H., Lipschutz, J. H., Nihalani, D., Perkins, B. D., & Lobo, G. P. (2020) · doi:10.20944/preprints202003.0296.v1 · Cited by 20
Disparate expression specificities coded by a shared Hox-C enhancer
Miller, S. W., & Posakony, J. W. (2020) · eLife · doi:10.7554/elife.39876 · Cited by 4
Zebrafish relatives as models for functional comparative genetics and genomics
Podobnik, M., Lu, Y., Irion, U., & Currie, P. D. (2026) · Nature Reviews Genetics · doi:10.1038/s41576-026-01007-4 · Cited by 1
Metadata
- Authors
- Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K.
- Affiliations
- Massachusetts General Hospital (US)
- Harvard University (US)
- Broad Institute (US)
- Corresponding author
- Yeh, J-R Joanna
- Joung, J Keith
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2013-01-29
- Volume
- 31
- Issue
- 3
- Pages
- 227-229
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC3686313
- OpenAlex
- W1976391205
- Cited by
- 2,937
- References
- 24
- Citations by year
- 2026: 68 · 2025: 125 · 2024: 146 · 2023: 159 · 2022: 207 · 2021: 203 · 2020: 227 · 2019: 230 · 2018: 252 · 2017: 246 · 2016: 306 · 2015: 276 · 2014: 300 · 2013: 189
- FWCI
- 160.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- National Institute on Aging (K01AG031300)
- National Human Genome Research Institute (P50HG005550)
- National Cancer Institute (R01CA140188)
- National Institute of General Medical Sciences (DP1GM105378, R01GM088040, F32GM105189)
- National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- CRISPR-Cas, genome editing, zebrafish embryos, Cas9 endonuclease, guide RNA, zinc finger nucleases, TALEN
- MeSH terms
- Animals, Base Sequence, DNA, Embryo, Nonmammalian, Endonucleases, Genetic Engineering, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Zebrafish, Genome, RNA Editing, DNA Cleavage, Inverted Repeat Sequences, RNA, Small Untranslated, CRISPR-Cas Systems
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