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Efficient genome editing in zebrafish using a CRISPR-Cas system
Open accessAbstract
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
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“Also in January 2013, similar publications came out from the laboratories of Jennifer Doudna (Berkeley College, USA) [36], Jin-Soo Kim (Seoul University, South Korea) [37] and J. Keith Joung (Harvard School of Medicine, USA) [38].”
From CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing (2022) · cites this paper as [38]
Experimental — accuracy not yet measuredIn the citing paper
“Recently, the CRISPR/Cas system has been successfully applied to human cells19,20,21,22 and several model organisms for genome editing.23,24,25,26”
From The CRISPR/Cas9 system facilitates clearance of the intrahepatic HBV templates in vivo (2014) · cites this paper as [24]
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Metadata
- Authors
- Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K.
- Affiliations
- Massachusetts General Hospital (US)
- Harvard University (US)
- Broad Institute (US)
- Corresponding author
- Yeh, J-R Joanna
- Joung, J Keith
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2013-01-29
- Volume
- 31
- Issue
- 3
- Pages
- 227-229
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC3686313
- OpenAlex
- W1976391205
- Cited by
- 2,937
- References
- 24
- Citations by year
- 2026: 68 · 2025: 125 · 2024: 146 · 2023: 159 · 2022: 207 · 2021: 203 · 2020: 227 · 2019: 230 · 2018: 252 · 2017: 246 · 2016: 306 · 2015: 276 · 2014: 300 · 2013: 189
- FWCI
- 160.03
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- Funders
- National Institute on Aging (K01AG031300)
- National Human Genome Research Institute (P50HG005550)
- National Cancer Institute (R01CA140188)
- National Institute of General Medical Sciences (DP1GM105378, R01GM088040, F32GM105189)
- National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- CRISPR-Cas, genome editing, zebrafish embryos, Cas9 endonuclease, guide RNA, zinc finger nucleases, TALEN
- MeSH terms
- Animals, Base Sequence, DNA, Embryo, Nonmammalian, Endonucleases, Genetic Engineering, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Zebrafish, Genome, RNA Editing, DNA Cleavage, Inverted Repeat Sequences, RNA, Small Untranslated, CRISPR-Cas Systems
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