Cited by 2,937CRISPR and Genetic EngineeringZebrafish Biomedical Research ApplicationsAnimal Genetics and Reproduction

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Efficient genome editing in zebrafish using a CRISPR-Cas system

Open access

Hwang, Woong Y, Fu, Yanfang, Reyon, Deepak, Maeder, Morgan L, Tsai, Shengdar Q, Sander, Jeffry D, Peterson, Randall T, Yeh, J-R Joanna, Joung, J Keith·2013·Nature Biotechnology

DOI 10.1038/nbt.2501PMC PMC3686313

Open this paper in CoChat — free account

Abstract

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nbt.2501

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
CRISPR/Cas, the Immune System of Bacteria and Archaea
Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471
Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes
Brouns, S. J. J., Jore, M. M., Lundgren, M., Westra, E. R., Slijkhuis, R. J. H., Snijders, A. P. L., Dickman, M. J., Makarova, K. S., Koonin, E. V., & van der Oost, J. (2008) · Science · doi:10.1126/science.1159689 · Cited by 2,460
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
RNA-guided genetic silencing systems in bacteria and archaea
Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012) · Nature · doi:10.1038/nature10886 · Cited by 1,851

Show all 24 references

Sorted by citations

Items 901–1,000 of 1,007

Variant Intronic Enhancer Controls SCN10A-short Expression and Heart Conduction
Man, J. C. K., Bosada, F. M., Scholman, K. T., Offerhaus, J. A., Walsh, R., van Duijvenboden, K., van Eif, V. W. W., Bezzina, C. R., Verkerk, A. O., Boukens, B. J., et al. (2021) · Circulation · doi:10.1161/circulationaha.121.054083 · Cited by 47
CRISPR/Cas9-Mediated Gene Therapy for Glioblastoma: A Scoping Review
Begagić, E., Bečulić, H., Đuzić, N., Džidić-Krivić, A., Pugonja, R., Muharemović, A., Jaganjac, B., Salković, N., Sefo, H., & Pojskić, M. (2024) · Biomedicines · doi:10.3390/biomedicines12010238 · Cited by 55
Targeted mutagenesis of aryl hydrocarbon receptor 2a and 2b genes in Atlantic killifish (Fundulus heteroclitus)
Aluru, N., Karchner, S. I., Franks, D. G., Nacci, D. E., Champlin, D. M., & Hahn, M. E. (2014) · Aquatic Toxicology · doi:10.1016/j.aquatox.2014.11.016 · Cited by 47
Regulation of Cell Polarity and RNA Localization in Vertebrate Oocytes
Houston, D. W. (2013) · International review of cytology · doi:10.1016/b978-0-12-407694-5.00004-3 · Cited by 47
Optimised metrics for CRISPR-KO screens with second-generation gRNA libraries
Ong, S. H., Li, Y., Koike-Yusa, H., & Yusa, K. (2017) · Scientific Reports · doi:10.1038/s41598-017-07827-z · Cited by 47
Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells
Voets, O., Tielen, F., Elstak, E., Benschop, J., Grimbergen, M., Stallen, J., Janssen, R., van Marle, A., & Essrich, C. (2017) · PLOS ONE · doi:10.1371/journal.pone.0182974 · Cited by 48
CRISPR in Animals and Animal Models
Shrock, E., & Güell, M. (2017) · Progress in Molecular Biology and Translational Science · doi:10.1016/bs.pmbts.2017.07.010 · Cited by 45
Use of CRISPR/Cas9 gene-editing tools for developing models in drug discovery
Ahmad, G., & Amiji, M. M. (2018) · Drug Discovery Today · doi:10.1016/j.drudis.2018.01.014 · Cited by 48
Hif-1α and Hif-2α regulate hemogenic endothelium and hematopoietic stem cell formation in zebrafish
Gerri, C., Marass, M., Rossi, A., & Stainier, D. Y. R. (2018) · Blood · doi:10.1182/blood-2017-07-797795 · Cited by 47
Generation of albino medaka (Oryzias latipes) by CRISPR/Cas9
Fang, J., Chen, T., Pan, Q., & Wang, Q. (2018) · Journal of Experimental Zoology Part B Molecular and Developmental Evolution · doi:10.1002/jez.b.22808 · Cited by 47
Zebrafish as an Emerging Model for Bioassay-Guided Natural Product Drug Discovery for Neurological Disorders
Pitchai, A., Rajaretinam, R. K., & Freeman, J. L. (2019) · Medicines · doi:10.3390/medicines6020061 · Cited by 49
First report on CRISPR/Cas9-targeted mutagenesis in the Colorado potato beetle, Leptinotarsa decemlineata
Gui, S., Taning, C. N. T., Wei, D., & Smagghe, G. J. (2020) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2020.104013 · Cited by 47
Genetic Engineering of Zebrafish in Cancer Research
Raby, L., Völkel, P., Bourhis, X. L., & Angrand, P. (2020) · Cancers · doi:10.3390/cancers12082168 · Cited by 51
Efficient genome editing in pathogenic mycobacteria using Streptococcus thermophilus CRISPR1-Cas9
Meijers, A. S., Troost, R., Ummels, R., Maaskant, J. J., Speer, A., Nejentsev, S., Bitter, W., & Kuijl, C. P. (2020) · Tuberculosis · doi:10.1016/j.tube.2020.101983 · Cited by 47
Production of Germ Cell-Less Rainbow Trout by dead end Gene Knockout and their Use as Recipients for Germ Cell Transplantation
Fujihara, R., Katayama, N., Sadaie, S., Miwa, M., Matias, G. A. S., Ichida, K., Fujii, W., Naito, K., Hayashi, M., & Yoshizaki, G. (2022) · Marine Biotechnology · doi:10.1007/s10126-022-10128-w · Cited by 47
Knockout of the odorant receptor co-receptor, orco, impairs feeding, mating and egg-laying behavior in the fall armyworm Spodoptera frugiperda
Sun, H., Bu, L., Su, S., Guo, D., Gao, C., & Wu, S. (2022) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2022.103889 · Cited by 46
Building a zebrafish toolkit for investigating the pathobiology of epilepsy and identifying new treatments for epileptic seizures
Cunliffe, V. T. (2015) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2015.07.015 · Cited by 46
Effective heritable gene knockdown in zebrafish using synthetic microRNAs
Giacomotto, J., Rinkwitz, S., & Becker, T. S. (2015) · Nature Communications · doi:10.1038/ncomms8378 · Cited by 46
Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression
Kabadi, A. M., & Gersbach, C. A. (2014) · Methods · doi:10.1016/j.ymeth.2014.06.014 · Cited by 50
A transgenic zebrafish model for monitoring glucocorticoid receptor activity
Krug, R. G., Poshusta, T. L., Skuster, K. J., Berg, M. R., Gardner, S. L., & Clark, K. J. (2014) · Genes Brain & Behavior · doi:10.1111/gbb.12135 · Cited by 46
Identification of Wnt Genes Expressed in Neural Progenitor Zones during Zebrafish Brain Development
Duncan, R. N., Panahi, S., Piotrowski, T., & Dorsky, R. I. (2015) · PLoS ONE · doi:10.1371/journal.pone.0145810 · Cited by 46
Toward an Integrated Zebrafish Health Management Program Supporting Cancer and Neuroscience Research
Martins, S., Monteiro, J. F., Vito, M., Weintraub, D., Almeida, J., & Certal, A. C. (2016) · Zebrafish · doi:10.1089/zeb.2015.1198 · Cited by 46
Rosa26-targeted sheep gene knock-in via CRISPR-Cas9 system
Wu, M., Wei, C., Lian, Z., Liu, R., Zhu, C., Wang, H., Cao, J., Shen, Y., Zhao, F., Zhang, L., et al. (2016) · Scientific Reports · doi:10.1038/srep24360 · Cited by 46
Generation of Zebrafish Models by CRISPR/Cas9 Genome Editing
Hruscha, A., & Schmid, B. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-2152-2_24 · Cited by 46
CRISPR/Cas9-mediated mutation ofPHEXin rabbit recapitulates human X-linked hypophosphatemia (XLH)
Sui, T., Yuan, L., Liu, H., Chen, M., Deng, J., Wang, Y., Li, Z., & Lai, L. (2016) · Human Molecular Genetics · doi:10.1093/hmg/ddw125 · Cited by 46
EphrinB1/EphB3b Coordinate Bidirectional Epithelial-Mesenchymal Interactions Controlling Liver Morphogenesis and Laterality
Cayuso, J., Dzementsei, A., Fischer, J. C., Karemore, G., Caviglia, S., Bartholdson, J., Wright, G. J., & Ober, E. A. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.10.009 · Cited by 46
Modeling sleep and neuropsychiatric disorders in zebrafish
Levitas‐Djerbi, T., & Appelbaum, L. (2017) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2017.02.017 · Cited by 46
Detecting and typing target DNA with a novel CRISPR-typing PCR (ctPCR) technique
Zhang, B., Xia, Q., Wang, Q., Xia, X., & Wang, J. (2018) · Analytical Biochemistry · doi:10.1016/j.ab.2018.09.012 · Cited by 46
A Comparative Perspective on Brain Regeneration in Amphibians and Teleost Fish
Lust, K., & Tanaka, E. M. (2019) · Developmental Neurobiology · doi:10.1002/dneu.22665 · Cited by 51
The Zebrafish as an Emerging Model to Study DNA Damage in Aging, Cancer and Other Diseases
Cayuela, M. L., Claes, K., Ferreira, M. G., Henriques, C. M., Eeden, F. V., Varga, M., Vierstraete, J., & Mione, M. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2018.00178 · Cited by 50
Wnt signaling mediates new nephron formation during zebrafish kidney regeneration
Kamei, C. N., Gallegos, T. F., Liu, Y., Hukriede, N. A., & Drummond, I. A. S. (2019) · Development · doi:10.1242/dev.168294 · Cited by 46
There Is Something Fishy About Liver Cancer: Zebrafish Models of Hepatocellular Carcinoma
Wrighton, P. J., Oderberg, I. M., & Goessling, W. (2019) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2019.05.002 · Cited by 44
Moving toward genome-editing therapies for cardiovascular diseases
Musunuru, K. (2022) · Journal of Clinical Investigation · doi:10.1172/jci148555 · Cited by 48
Oceans of opportunity: Exploring vertebrate hematopoiesis in zebrafish
Carroll, K. J., & North, T. E. (2014) · Experimental Hematology · doi:10.1016/j.exphem.2014.05.002 · Cited by 47
A CRISPR method for genome engineering
Wilkinson, R. A., & Wiedenheft, B. (2014) · F1000Prime Reports · doi:10.12703/p6-3 · Cited by 46
Genome editing in zebrafish: a practical overview
Sertori, R., Trengove, M. C., Basheer, F., Ward, A. C., & Liongue, C. (2015) · Briefings in Functional Genomics · doi:10.1093/bfgp/elv051 · Cited by 44
Repulsive cues combined with physical barriers and cell–cell adhesion determine progenitor cell positioning during organogenesis
Paksa, A., Bandemer, J., Höckendorf, B., Razin, N., Tarbashevich, K., Minina, S., Meyen, D., Biundo, A., Leidel, S. A., Peyriéras, N., et al. (2016) · Nature Communications · doi:10.1038/ncomms11288 · Cited by 45
Targeted genome editing in the rare actinomycete Actinoplanes sp. SE50/110 by using the CRISPR/Cas9 System
Wolf, T., Gren, T., Thieme, E., Wibberg, D., Zemke, T., Pühler, A., & Kalinowski, J. (2016) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2016.05.039 · Cited by 44
Genome editing revolutionize the creation of genetically modified pigs for modeling human diseases
Yao, J. T., Huang, J., & Zhao, J. (2016) · Human Genetics · doi:10.1007/s00439-016-1710-6 · Cited by 47
Heterotypic interactions regulate cell shape and density during color pattern formation in zebrafish
Mahalwar, P., Singh, A. P., Fadeev, A., Nüsslein-Volhard, C., & Irion, U. (2016) · Biology Open · doi:10.1242/bio.022251 · Cited by 45
rbpms2 functions in Balbiani body architecture and ovary fate
Kaufman, O. H., Lee, K., Martin, M., Rothhämel, S., & Marlow, F. L. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007489 · Cited by 45
Modeling Niemann-Pick disease type C1 in zebrafish: a robust platform for in vivo screening of candidate therapeutic compounds
Tseng, W.-C., Loeb, H. E., Pei, W., Tsai‐Morris, C., Xu, L., Cluzeau, C. V. M., Wassif, C. A., Feldman, B., Burgess, S. M., Pavan, W. J., et al. (2018) · Disease Models & Mechanisms · doi:10.1242/dmm.034165 · Cited by 45
Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii
Ahmed, H. M. M., Hildebrand, L., & Wimmer, E. A. (2019) · BMC Biotechnology · doi:10.1186/s12896-019-0588-5 · Cited by 45
A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy
Matsumoto, D., Tamamura, H., & Nomura, W. (2020) · Communications Biology · doi:10.1038/s42003-020-01340-2 · Cited by 46
Disease modeling by efficient genome editing using a near PAM-less base editor in vivo
Rosello, M., Serafini, M., Mignani, L., Finazzi, D., Giovannangeli, C., Mione, M., Concordet, J., & Bene, F. D. (2022) · Nature Communications · doi:10.1038/s41467-022-31172-z · Cited by 46
Zebrafish Models of Cerebrovascular Disease
Walcott, B. P., & Peterson, R. T. (2014) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/jcbfm.2014.27 · Cited by 49
PADS Arsenal: a database of prokaryotic defense systems related genes
Zhang, Y., Zhang, Z., Zhang, H., Zhao, Y., Zhang, Z., & Xiao, J. (2019) · Nucleic Acids Research · doi:10.1093/nar/gkz916 · Cited by 46
Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease
Chaoul, V., Dib, E., Bedran, J., Khoury, C., Shmoury, O., Harb, F., & Soueid, J. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241914898 · Cited by 47
Genetic basis of octanoic acid resistance in Drosophila sechellia : functional analysis of a fine‐mapped region
López, J. M. A., Lanno, S., Auerbach, J. M., Moskowitz, E. C., Sligar, L. A., Wittkopp, P. J., & Coolon, J. D. (2016) · Molecular Ecology · doi:10.1111/mec.14001 · Cited by 50
Exploiting CRISPR/Cas: Interference Mechanisms and Applications
Richter, H., Randau, L., & Plagens, A. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms140714518 · Cited by 46
Identifying Novel Cancer Therapies Using Chemical Genetics and Zebrafish
Dang, M., Fogley, R. D., & Zon, L. I. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-30654-4_5 · Cited by 47
nr0b1 (DAX1) mutation in zebrafish causes female-to-male sex reversal through abnormal gonadal proliferation and differentiation
Chen, S., Zhang, H., Wang, F., Zhang, W., & Peng, G. (2016) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2016.06.005 · Cited by 46
CAR1deletion by CRISPR/Cas9 reduces formation of ethyl carbamate from ethanol fermentation bySaccharomyces cerevisiae
Chin, Y., Kang, W.-K., Jang, H. W., Turner, T. L., & Kim, H. J. (2016) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-016-1831-x · Cited by 45
interleukin-11 induces and maintains progenitors of different cell lineages during Xenopus tadpole tail regeneration
Tsujioka, H., Kunieda, T., Katou, Y., Shirahige, K., Fukazawa, T., & Kubo, T. (2017) · Nature Communications · doi:10.1038/s41467-017-00594-5 · Cited by 45
Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish
Hu, P., Zhao, X., Zhang, Q., Li, W., & Zu, Y. (2018) · G3 Genes Genomes Genetics · doi:10.1534/g3.117.300359 · Cited by 44
Sequential specification of oligodendrocyte lineage cells by distinct levels of Hedgehog and Notch signaling
Ravanelli, A. M., Kearns, C. A., Powers, R. K., Wang, Y., Hines, J. H., Donaldson, M. J., & Appel, B. (2018) · Developmental Biology · doi:10.1016/j.ydbio.2018.10.004 · Cited by 43
A Regeneration Toolkit
Mokalled, M. H., & Poss, K. D. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.10.015 · Cited by 44
The zebrafish: A research model to understand the evolution of vertebrate immunity
García‐Moreno, D., Tyrkalska, S. D., Valera, A., Gómez‐Abenza, E., Pérez‐Oliva, A. B., & Mulero, V. (2019) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2019.04.067 · Cited by 47
CRISPR-Cas9 human gene replacement and phenomic characterization in Caenorhabditis elegans to understand the functional conservation of human genes and decipher variants of uncertain significance
McDiarmid, T. A., Au, V., Loewen, A. D., Liang, J., Mizumoto, K., Moerman, D. G., & Rankin, C. H. (2018) · Disease Models & Mechanisms · doi:10.1242/dmm.036517 · Cited by 50
BMP Signaling Gradient Scaling in the Zebrafish Pectoral Fin
Mateus, R., Holtzer, L., Seum, C., Hadjivasiliou, Z., Dubois, M., Jülicher, F., & González‐Gaitán, M. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.03.024 · Cited by 44
The Role of Zebrafish and Laboratory Rodents in Schizophrenia Research
Langová, V., Valeš, K., Horká, P., & Horáček, J. (2020) · Frontiers in Psychiatry · doi:10.3389/fpsyt.2020.00703 · Cited by 45
Development of a base editor for protein evolution via in situ mutation in vivo
Hao, W., Cui, W., Cheng, Z., Han, L., Suo, F., Liu, Z., Zhou, L., & Zhou, Z. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab673 · Cited by 43
PxTret1-like Affects the Temperature Adaptability of a Cosmopolitan Pest by Altering Trehalose Tissue Distribution
Zhou, H., Lei, G., Chen, Y., You, M., & You, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23169019 · Cited by 43
Engineered mRNA Delivery Systems for Biomedical Applications
Wang, J., Zhu, H., Gan, J., Liang, G., Li, L., & Zhao, Y. (2023) · Advanced Materials · doi:10.1002/adma.202308029 · Cited by 46
Maternal consumption of non‐toxic Microcystis by Daphnia magna induces tolerance to toxic Microcystis in offspring
Lyu, K., Guan, H., Wu, C., Wang, X., Wilson, A. E., & Yang, Z. (2015) · Freshwater Biology · doi:10.1111/fwb.12695 · Cited by 43
Antisense Oligonucleotide-Mediated Transcript Knockdown in Zebrafish
Pauli, A., Montague, T. G., Lennox, K. A., Behlke, M. A., & Schier, A. F. (2015) · PLoS ONE · doi:10.1371/journal.pone.0139504 · Cited by 42
A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism
Barzi, M., Pankowicz, F. P., Zorman, B., Liu, X., Legras, X. D., Yang, D., Borowiak, M., Bissig-Choisat, B., Sumazin, P., Li, F., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00049-x · Cited by 43
Addressing challenges in the clinical applications associated with CRISPR/Cas9 technology and ethical questions to prevent its misuse
Kang, X., Caparas, C. I. N., Soh, B.-S., & Fan, Y. (2017) · Protein & Cell · doi:10.1007/s13238-017-0477-4 · Cited by 49
Zebrafish Models of Photoreceptor Dysfunction and Degeneration
Noel, N. C. L., MacDonald, I. M., & Allison, W. T. (2021) · Biomolecules · doi:10.3390/biom11010078 · Cited by 44
Modulation of bioelectric cues in the evolution of flying fishes
Daane, J. M., Blum, N., Lanni, J. S., Boldt, H., Iovine, M. K., Higdon, C. W., Johnson, S. L., Lovejoy, N. R., & Harris, M. P. (2021) · Current Biology · doi:10.1016/j.cub.2021.08.054 · Cited by 42
Developments in our understanding of the genetic basis of birth defects
Webber, D. M., MacLeod, S. L., Bamshad, M. J., Shaw, G. M., Finnell, R. H., Shete, S. S., Witte, J. S., Erickson, S. W., Murphy, L. D., & Hobbs, C. A. (2015) · Birth Defects Research Part A Clinical and Molecular Teratology · doi:10.1002/bdra.23385 · Cited by 44
Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System
Yin, L., Jao, L.-E., & Chen, W. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2917-7_16 · Cited by 43
The Study of Glioma by Xenotransplantation in Zebrafish Early Life Stages
Vittori, M., Motaln, H., & Turnšek, T. L. (2015) · Journal of Histochemistry & Cytochemistry · doi:10.1369/0022155415595670 · Cited by 42
A Short Splice Form of Xin-Actin Binding Repeat Containing 2 (XIRP2) Lacking the Xin Repeats Is Required for Maintenance of Stereocilia Morphology and Hearing Function
Francis, S. P., Krey, J. F., Krystofiak, E., Cui, R., Nanda, S., Xu, W., Kachar, B., Gillespie, P. G., & Shin, J. (2015) · Journal of Neuroscience · doi:10.1523/jneurosci.3449-14.2015 · Cited by 42
Identification of Proteins Associated with an IFNγ-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR
Fujita, T., & Fujii, H. (2014) · PLoS ONE · doi:10.1371/journal.pone.0103084 · Cited by 42
Dual-reporter surrogate systems for efficient enrichment of genetically modified cells
Ren, C., Xu, K., Liu, Z., Shen, J., Han, F., Chen, Z., & Zhang, Z. (2015) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-015-1874-6 · Cited by 42
Gene editing nuclease and its application in tilapia
Li, M., & Wang, D. (2017) · 中国科学通报：英文版 · doi:10.1016/j.scib.2017.01.003 · Cited by 42
CRISPR/Cas9-mediated correction of human genetic disease
Men, K., Duan, X., He, Z., Yang, Y., Yao, S., & Wei, Y. (2017) · Science China Life Sciences · doi:10.1007/s11427-017-9032-4 · Cited by 44
How many roads lead to cohesinopathies?
Banerji, R., Skibbens, R. V., & Iovine, M. K. (2017) · Developmental Dynamics · doi:10.1002/dvdy.24510 · Cited by 40
Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells
Luo, W., Galvan, D. L., Woodard, L. E., Dorset, D. C., Levy, S. A., & Wilson, M. H. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx572 · Cited by 42
High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion
Momose, T., Cian, A. D., Shiba, K., Inaba, K., Giovannangeli, C., & Concordet, J. (2018) · Scientific Reports · doi:10.1038/s41598-018-30188-0 · Cited by 42
Neutrophil plays critical role during Edwardsiella piscicida immersion infection in zebrafish larvae
Wang, Z., Lin, L., Chen, W., Zheng, X., Zhang, Y., Liu, Q., & Yang, D. (2019) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2019.02.008 · Cited by 42
CRISPR Genome Editing Systems in the Genus Clostridium : a Timely Advancement
McAllister, K. N., & Sorg, J. A. (2019) · Journal of Bacteriology · doi:10.1128/jb.00219-19 · Cited by 49
Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans
Xu, X., Wang, Y., Bi, H., Xu, J. Q., Liu, Z., Niu, C., He, L., James, A. A., Li, K., & Huang, Y. (2019) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2019.103243 · Cited by 42
Targeted knockout of GABA receptor gamma 2 subunit provokes transient light-induced reflex seizures in zebrafish larvae
Liao, M., Kundap, U. P., Rosch, R. E., Burrows, D. R. W., Meyer, M. P., Bencheikh, B. O. A., Cossette, P., & Samarut, É. (2019) · Disease Models & Mechanisms · doi:10.1242/dmm.040782 · Cited by 42
Precise base editing for the in vivo study of developmental signaling and human pathologies in zebrafish
Rosello, M., Vougny, J., Czarny, F., Mione, M., Concordet, J., Albadri, S., & Bene, F. D. (2021) · eLife · doi:10.7554/elife.65552 · Cited by 44
CRISPR-Cas12a induced DNA double-strand breaks are repaired by multiple pathways with different mutation profiles in Magnaporthe oryzae
Huang, J. T., Rowe, D., Subedi, P., Zhang, W., Suelter, T., Valent, B. S., & Cook, D. E. (2022) · Nature Communications · doi:10.1038/s41467-022-34736-1 · Cited by 41
Transplantation in zebrafish
Gansner, J. M., Dang, M., Ammerman, M., & Zon, L. I. (2016) · Methods in cell biology · doi:10.1016/bs.mcb.2016.08.006 · Cited by 41
Defining the gene repertoire and spatiotemporal expression profiles of adhesion G protein-coupled receptors in zebrafish
Harty, B. L., Krishnan, A., Sanchez, N., Schiöth, H. B., & Monk, K. R. (2015) · BMC Genomics · doi:10.1186/s12864-015-1296-8 · Cited by 45
CrisprGE: a central hub of CRISPR/Cas-based genome editing
Kaur, K., Tandon, H., Gupta, A., & Kumar, M. (2015) · Database · doi:10.1093/database/bav055 · Cited by 41
Cas9-Based Genome Editing in Drosophila
Housden, B. E., Lin, S., & Perrimon, N. (2014) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-801185-0.00019-2 · Cited by 41
Corticotropin-releasing hormone: Mediator of vertebrate life stage transitions?
Watanabe, Y., Grommen, S. V. H., & Groef, B. D. (2016) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2016.02.012 · Cited by 41
Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish
Ota, S., Taimatsu, K., Yanagi, K., Namiki, T., Ohga, R., Higashijima, S., & Kawahara, A. (2016) · Scientific Reports · doi:10.1038/srep34991 · Cited by 41
Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis
Lee, R. T. H., Ng, A. S. M., & Ingham, P. W. (2016) · PLoS ONE · doi:10.1371/journal.pone.0166020 · Cited by 41
CRISPR/Cas9 mediated chicken Stra8 gene knockout and inhibition of male germ cell differentiation
Zhang, Y., Wang, Y., Zuo, Q., Li, D., Zhang, W., Wang, F., Ji, Y., Jin, J., Lu, Z., Wang, M., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0172207 · Cited by 40
Gene editing as applied to prevention of reproductive porcine reproductive and respiratory syndrome
Whitworth, K. M., & Prather, R. S. (2017) · Molecular Reproduction and Development · doi:10.1002/mrd.22811 · Cited by 40
CRISPR/Cas9: at the cutting edge of hepatology
Pankowicz, F. P., Jarrett, K. E., Lagor, W. R., & Bissig, K. (2017) · Gut · doi:10.1136/gutjnl-2016-313565 · Cited by 43
Multifunctional nucleic acid nanostructures for gene therapies
Liu, J., Wang, Z., Zhao, S., & Ding, B. (2018) · Nano Research · doi:10.1007/s12274-018-2093-x · Cited by 41
Combined Computational–Experimental Approach to Explore the Molecular Mechanism of SaCas9 with a Broadened DNA Targeting Range
Luan, B., Xu, G., Feng, M., Cong, L., & Zhou, R. (2019) · Journal of the American Chemical Society · doi:10.1021/jacs.8b13144 · Cited by 42
Proline-rich protein PRR19 functions with cyclin-like CNTD1 to promote meiotic crossing over in mouse
Bondarieva, A., Raveendran, K., Telychko, V., Rao, H. B. D. P., Ravindranathan, R., Zorzompokou, C., Finsterbusch, F., Dereli, I., Papanikos, F., Tränkner, D., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-16885-3 · Cited by 39

Authors: Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K.
Affiliations: Massachusetts General Hospital (US)
Harvard University (US)
Broad Institute (US)
Corresponding author: Yeh, J-R Joanna
Joung, J Keith
ORCID: Hwang, Woong Y
Reyon, Deepak
Maeder, Morgan L
Tsai, Shengdar Q
Sander, Jeffry D
Peterson, Randall T
Joung, J Keith
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2013-01-29
Volume: 31
Issue: 3
Pages: 227-229
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.2501
PMC: PMC3686313
OpenAlex: W1976391205
Cited by: 2,937
References: 24
Citations by year: 2026: 68 · 2025: 125 · 2024: 146 · 2023: 159 · 2022: 207 · 2021: 203 · 2020: 227 · 2019: 230 · 2018: 252 · 2017: 246 · 2016: 306 · 2015: 276 · 2014: 300 · 2013: 189
FWCI: 160.03
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
Funders: National Institute on Aging (K01AG031300)
National Human Genome Research Institute (P50HG005550)
National Cancer Institute (R01CA140188)
National Institute of General Medical Sciences (DP1GM105378, R01GM088040, F32GM105189)
National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords: CRISPR-Cas, genome editing, zebrafish embryos, Cas9 endonuclease, guide RNA, zinc finger nucleases, TALEN
MeSH terms: Animals, Base Sequence, DNA, Embryo, Nonmammalian, Endonucleases, Genetic Engineering, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Zebrafish, Genome, RNA Editing, DNA Cleavage, Inverted Repeat Sequences, RNA, Small Untranslated, CRISPR-Cas Systems

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Efficient genome editing in zebrafish using a CRISPR-Cas system

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks