Cited by 2,937CRISPR and Genetic EngineeringZebrafish Biomedical Research ApplicationsAnimal Genetics and Reproduction

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

Efficient genome editing in zebrafish using a CRISPR-Cas system

Open access

Hwang, Woong Y, Fu, Yanfang, Reyon, Deepak, Maeder, Morgan L, Tsai, Shengdar Q, Sander, Jeffry D, Peterson, Randall T, Yeh, J-R Joanna, Joung, J Keith·2013·Nature Biotechnology

DOI 10.1038/nbt.2501PMC PMC3686313

Open this paper in CoChat — free account

Abstract

In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/nbt.2501

Cites

24 of 24 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
Barrangou, R., Fremaux, C., Deveau, H., Richards, M., Boyaval, P., Moineau, S., Romero, D. A., & Horvath, P. (2007) · Science · doi:10.1126/science.1138140 · Cited by 5,947
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
Gasiunas, G., Barrangou, R., Horvath, P., & Siksnys, V. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1208507109 · Cited by 2,769
CRISPR/Cas, the Immune System of Bacteria and Archaea
Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471
Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes
Brouns, S. J. J., Jore, M. M., Lundgren, M., Westra, E. R., Slijkhuis, R. J. H., Snijders, A. P. L., Dickman, M. J., Makarova, K. S., Koonin, E. V., & van der Oost, J. (2008) · Science · doi:10.1126/science.1159689 · Cited by 2,460
Genome editing with engineered zinc finger nucleases
Urnov, F. D., Rebar, E. J., Holmes, M. C., Zhang, H. S., & Gregory, P. D. (2010) · Nature Reviews Genetics · doi:10.1038/nrg2842 · Cited by 2,285
RNA-guided genetic silencing systems in bacteria and archaea
Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012) · Nature · doi:10.1038/nature10886 · Cited by 1,851

Flagged first, then by citations

Show all 24 references

Sorted by citations

Items 501–600 of 1,007

Development of both type I–B and type II CRISPR/Cas genome editing systems in the cellulolytic bacterium Clostridium thermocellum
Walker, J. E., Lanahan, A. A., Zheng, T., Toruno, C., Lynd, L. R., Cameron, J. C., Olson, D. G., & Eckert, C. A. (2019) · Metabolic Engineering Communications · doi:10.1016/j.mec.2019.e00116 · Cited by 101
Beyond genome sequencing: Lineage tracking with barcodes to study the dynamics of evolution, infection, and cancer
Blundell, J. R., & Levy, S. F. (2014) · Genomics · doi:10.1016/j.ygeno.2014.09.005 · Cited by 98
Advancing chimeric antigen receptor T cell therapy with CRISPR/Cas9
Ren, J., & Zhao, Y. (2017) · Protein & Cell · doi:10.1007/s13238-017-0410-x · Cited by 103
Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes
Papanikos, F., Clément, J. A. J., Testa, E., Ravindranathan, R., Grey, C., Dereli, I., Bondarieva, A., Valerio-Cabrera, S., Stanzione, M., Schleiffer, A., et al. (2019) · Molecular Cell · doi:10.1016/j.molcel.2019.03.022 · Cited by 99
Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes
Matsumura, K., Seiriki, K., Okada, S., Nagase, M., Ayabe, S., Yamada, I., Furuse, T., Shibuya, H., Yasuda, Y., Yamamori, H., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-14697-z · Cited by 107
Knockout crickets for the study of learning and memory: Dopamine receptor Dop1 mediates aversive but not appetitive reinforcement in crickets
Awata, H., Watanabe, T., Hamanaka, Y., Mito, T., Noji, S., & Mizunami, M. (2015) · Scientific Reports · doi:10.1038/srep15885 · Cited by 98
Precision Targeted Mutagenesis via Cas9 Paired Nickases in Rice
Mikami, M., Toki, S., & Endo, M. (2016) · Plant and Cell Physiology · doi:10.1093/pcp/pcw049 · Cited by 97
CRISPR/Cas9: An RNA‐guided highly precise synthetic tool for plant genome editing
Demirci, Y., Zhang, B., & Ünver, T. (2017) · Journal of Cellular Physiology · doi:10.1002/jcp.25970 · Cited by 103
Pyridoxine-Dependent Epilepsy in Zebrafish Caused by Aldh7a1 Deficiency
Pena, I. A., Roussel, Y., Daniel, K., Mongeon, K., Johnstone, D. L., Mendes, H. W., Bosma, M., Saxena, V., Lepage, N., Chakraborty, P. K., et al. (2017) · Genetics · doi:10.1534/genetics.117.300137 · Cited by 98
Temporally coordinated signals progressively pattern the anteroposterior and dorsoventral body axes
Tuazon, F. B., & Mullins, M. C. (2015) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2015.06.003 · Cited by 98
Evidence for the widespread distribution of CRISPR-Cas system in the PhylumCyanobacteria
Cai, F., Axen, S. D., & Kerfeld, C. A. (2013) · RNA Biology · doi:10.4161/rna.24571 · Cited by 96
Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9
Ma, Y., Shen, B., Zhang, X., Lu, Y., Chen, W., Ma, J., Huang, X., & Zhang, L. (2014) · PLoS ONE · doi:10.1371/journal.pone.0089413 · Cited by 97
CRISPR/Cas System and Factors Affecting Its Precision and Efficiency
Javaid, N., & Choi, S. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.761709 · Cited by 106
Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice
Mikami, M., Toki, S., & Endo, M. (2015) · Plant Cell Reports · doi:10.1007/s00299-015-1826-5 · Cited by 97
The Importance of Zebrafish in Biomedical Research
Tavares, B., & Lopes, S. S. (2013) · Acta Médica Portuguesa · doi:10.20344/amp.4628 · Cited by 99
Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9
Liu, Y., Ma, S., Wang, X., Chang, J., Gao, J., Shi, R., Zhang, J., Lü, W., Liu, Y., Zhao, P., et al. (2014) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2014.03.010 · Cited by 96
Gap junctions composed of connexins 41.8 and 39.4 are essential for colour pattern formation in zebrafish
Irion, U., Frohnhöfer, H. G., Krauss, J., Çolak Champollion, T., Maischein, H.-M., Geiger-Rudolph, S., Weiler, C., & Nüsslein-Volhard, C. (2014) · eLife · doi:10.7554/elife.05125 · Cited by 91
Increased trabecular bone and improved biomechanics in an osteocalcin null rat model created by CRISPR/Cas9 technology
Lambert, L. J., Challa, A. K., Niu, A., Zhou, L., Tucholski, J., Johnson, M. S., Nagy, T. R., Eberhardt, A. W., Estep, P. N., Kesterson, R. A., et al. (2016) · Disease Models & Mechanisms · doi:10.1242/dmm.025247 · Cited by 94
Small teleost fish provide new insights into human skeletal diseases
Witten, P. E., Harris, M. P., Huysseune, A., & Winkler, C. (2016) · Methods in cell biology · doi:10.1016/bs.mcb.2016.09.001 · Cited by 97
Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans
Katic, I., & Großhans, H. (2013) · Genetics · doi:10.1534/genetics.113.155754 · Cited by 95
The Evolutionary Genomics of Cichlid Fishes: Explosive Speciation and Adaptation in the Postgenomic Era
Henning, F., & Meyer, A. (2014) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev-genom-090413-025412 · Cited by 96
A Review on Advanced CRISPR-Based Genome-Editing Tools: Base Editing and Prime Editing
Sichani, A. S., Ranjbar, M., Baneshi, M., Zadeh, F. T., & Fallahi, J. (2022) · Molecular Biotechnology · doi:10.1007/s12033-022-00639-1 · Cited by 105
A versatile framework for microbial engineering using synthetic non-coding RNAs
Qi, L. S., & Arkin, A. P. (2014) · Nature Reviews Microbiology · doi:10.1038/nrmicro3244 · Cited by 96
Autophagy activators suppress cystogenesis in an autosomal dominant polycystic kidney disease model
Zhu, P., Sieben, C. J., Xu, X., Harris, P. C., & Lin, X. (2016) · Human Molecular Genetics · doi:10.1093/hmg/ddw376 · Cited by 95
DIPA-CRISPR is a simple and accessible method for insect gene editing
Shirai, Y., Piulachs, M., Bellés, X., & Daimon, T. (2022) · Cell Reports Methods · doi:10.1016/j.crmeth.2022.100215 · Cited by 96
Engineering nucleases for gene targeting: safety and regulatory considerations
Pauwels, K., Podevin, N., Breyer, D., Carroll, D., & Herman, P. (2013) · New Biotechnology · doi:10.1016/j.nbt.2013.07.001 · Cited by 97
Oligonucleotide-based targeted gene editing in C. elegans via the CRISPR/Cas9 system
Zhao, P., Zhang, Z., Ke, H., Yue, Y., & Xue, D. (2014) · Cell Research · doi:10.1038/cr.2014.9 · Cited by 92
Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 ribonucleoprotein complexes
Meccariello, A., Monti, S. M., Romanelli, A., Colonna, R., Primo, P., Inghilterra, M. G., Corsano, G. D., Ramaglia, A., Iazzetti, G., Chiarore, A., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-10347-5 · Cited by 93
Puma, noxa, p53, and p63 differentially mediate stress pathway induced apoptosis
Wang, J., Thomas, H. R., Li, Z., Yeo, N. C., Scott, H. E., Dang, N. D.-P., Hossain, M. I., Andrabi, S. A., & Parant, J. M. (2021) · Cell Death and Disease · doi:10.1038/s41419-021-03902-6 · Cited by 98
Improvements in algal lipid production: a systems biology and gene editing approach
Banerjee, A., Banerjee, C., Negi, S., Chang, J., & Shukla, P. (2017) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2017.1356803 · Cited by 95
Recent Advances in CRISPR‐Cas9 Genome Editing Technology for Biological and Biomedical Investigations
Singh, V., Gohil, N., Ramirez-Garcia, R., Braddick, D., & Fofié, C. K. (2017) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26165 · Cited by 92
Effective CRISPR/Cas9-based nucleotide editing in zebrafish to model human genetic cardiovascular disorders
Tessadori, F., Roessler, H. I., Savelberg, S. M. C., Chocron, S., Kamel, S. M., Duran, K. J., van Haelst, M. M., van Haaften, G. W., & Bakkers, J. (2018) · Disease Models & Mechanisms · doi:10.1242/dmm.035469 · Cited by 98
PLPHP deficiency: clinical, genetic, biochemical, and mechanistic insights
Johnstone, D. L., Al-Shekaili, H. H., Tarailo‐Graovac, M., Wolf, N. I., Ivy, A. S., Demarest, S., Roussel, Y., Čiapaitė, J., van Roermund, C. W. T., Kernohan, K. D., et al. (2018) · Brain · doi:10.1093/brain/awy346 · Cited by 94
Engineering nanoparticle toolkits for mRNA delivery
Sun, B., Wu, W., Narasipura, E. A., Ma, Y., Yu, C., Fenton, O. S., & Song, H. (2023) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2023.115042 · Cited by 96
The three major types of CRISPR ‐ Cas systems function independently in CRISPR RNA biogenesis in S treptococcus thermophilus
Carte, J. M., Christopher, R. T., Smith, J. T., Olson, S., Barrangou, R., Moineau, S., Glover, C. V. C., Graveley, B. R., Terns, R. M., & Terns, M. P. (2014) · Molecular Microbiology · doi:10.1111/mmi.12644 · Cited by 95
BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment
Boel, A., Steyaert, W., Rocker, N. D., Menten, B., Callewaert, B. L., Paepe, A. D., Coucke, P. J., & Willaert, A. (2016) · Scientific Reports · doi:10.1038/srep30330 · Cited by 88
Engineering and modification of microbial chassis for systems and synthetic biology
Chi, H., Wang, X., Shao, Y., Qin, Y., Deng, Z., Wang, L., & Chen, S. (2018) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2018.12.001 · Cited by 91
Ultrafast tissue staining with chemical tags
Kohl, J., Ng, J., Cachero, S., Ciabatti, E., Dolan, M.-J., Sutcliffe, B., Tozer, A., Ruehle, S., Krueger, D., Frechter, S., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1411087111 · Cited by 91
Untitled work
Suresh (2017) · doi:10.1007/978-1-4939-6518-2_7 · Cited by 78
Genome editing in cardiovascular diseases
Strong, A., & Musunuru, K. (2016) · Nature Reviews Cardiology · doi:10.1038/nrcardio.2016.139 · Cited by 91
Depletion of juvenile hormone esterase extends larval growth in Bombyx mori
Zhang, Z., Liu, X., Shiotsuki, T., Wang, Z., Xu, X., Huang, Y., Li, M., Li, K., & Tan, A. (2017) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2017.01.001 · Cited by 90
Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy
Tokuhiro, K., & Dean, J. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.07.020 · Cited by 90
Editing streptomycete genomes in the CRISPR/Cas9 age
Alberti, F., & Corre, C. (2019) · Natural Product Reports · doi:10.1039/c8np00081f · Cited by 93
Advanced Echocardiography in Adult Zebrafish Reveals Delayed Recovery of Heart Function after Myocardial Cryoinjury
Hein, S. J., Lehmann, L., Kossack, M., Juergensen, L., Fuchs, D., Katus, H. A., & Hassel, D. (2015) · PLoS ONE · doi:10.1371/journal.pone.0122665 · Cited by 89
Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA
Mekler, V. M., Станиславович, М. Л., Semenova, E., Kuznedelov, K. D., & Severinov, K. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw138 · Cited by 93
Understanding functional miRNA–target interactions in vivo by site-specific genome engineering
Bassett, A. R., Azzam, G., Wheatley, L., Tibbit, C., Rajakumar, T., McGowan, S. J., Stanger, N., Ewels, P., Taylor, S., Ponting, C. P., et al. (2014) · Nature Communications · doi:10.1038/ncomms5640 · Cited by 88
The Developmental Genetics of Vertebrate Color Pattern Formation
Irion, U., Singh, A. P., & Nüsslein-Volhard, C. (2016) · Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2015.12.012 · Cited by 88
Efficient genome engineering approaches for the short-lived African turquoise killifish
Harel, I., Valenzano, D. R., & Brunet, A. (2016) · Nature Protocols · doi:10.1038/nprot.2016.103 · Cited by 89
A genetic switch for worker nutrition-mediated traits in honeybees
Roth, A., Vleurinck, C., Netschitailo, O., Bauer, V., Otte, M., Kaftanoğlu, O., Page, R. E., & Beye, M. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000171 · Cited by 87
Homing Endonucleases: From Genetic Anomalies to Programmable Genomic Clippers
Belfort, M., & Bonocora, R. P. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-968-0_1 · Cited by 87
Screening of mRNA Chemical Modification to Maximize Protein Expression with Reduced Immunogenicity
Uchida, S., Kataoka, K., & Itaka, K. (2015) · Pharmaceutics · doi:10.3390/pharmaceutics7030137 · Cited by 90
Systematic evaluation of CRISPR-Cas systems reveals design principles for genome editing in human cells
Wang, Y., Liu, K. I., Sutrisnoh, N.-A. B., Srinivasan, H., Zhang, J., Li, J., Zhang, F., Richard, J. L. C., Xing, H., Shanmugam, R., et al. (2018) · Genome biology · doi:10.1186/s13059-018-1445-x · Cited by 91
Discovering novel neuroactive drugs through high-throughput behavior-based chemical screening in the zebrafish
Bruni, G. N., Lakhani, P., & Kokel, D. (2014) · Frontiers in Pharmacology · doi:10.3389/fphar.2014.00153 · Cited by 89
Long-term drug administration in the adult zebrafish using oral gavage for cancer preclinical studies
Dang, M., Henderson, R. E., Garraway, L. A., & Zon, L. I. (2016) · Disease Models & Mechanisms · doi:10.1242/dmm.024166 · Cited by 87
Genome editing comes of age
Kim, J. (2016) · Nature Protocols · doi:10.1038/nprot.2016.104 · Cited by 89
Identification of a conserved and acute neurodegeneration‐specific microglial transcriptome in the zebrafish
Oosterhof, N., Holtman, I. R., Kuil, L. E., van der Linde, H. C., Boddeke, E., Eggen, B. J. L., & van Ham, T. J. (2016) · Glia · doi:10.1002/glia.23083 · Cited by 89
ARID1A loss in neuroblastoma promotes the adrenergic-to-mesenchymal transition by regulating enhancer-mediated gene expression
Shi, H., Tao, T., Abraham, B. J., Durbin, A. D., Zimmerman, M. W., Kadoch, C., & Look, A. T. (2020) · Science Advances · doi:10.1126/sciadv.aaz3440 · Cited by 87
One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
(2014) · Frontiers of Agricultural Science and Engineering · doi:10.15302/j-fase-2014007 · Cited by 86
The Zebrafish Heart as a Model of Mammalian Cardiac Function
Genge, C. E., Lin, E. C., Lee, L., Sheng, X., Rayani, K., Gunawan, M. G., Stevens, C. M., Li, A. Y., Shafaattalab, S., Claydon, T. W., et al. (2016) · Reviews of physiology, biochemistry and pharmacology · doi:10.1007/112_2016_5 · Cited by 86
A technological and regulatory outlook on CRISPR crop editing
Globus, R., & Qimron, U. (2017) · Journal of Cellular Biochemistry · doi:10.1002/jcb.26303 · Cited by 89
Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis
Rousseau, B. A., Hou, Z., Gramelspacher, M. J., & Zhang, Y. (2018) · Molecular Cell · doi:10.1016/j.molcel.2018.01.025 · Cited by 84
CRISPR/Cas9‐Directed Genome Editing of Cultured Cells
Yang, L., Yang, J. L., Byrne, S., Pan, J., & Church, G. M. (2014) · Current Protocols in Molecular Biology · doi:10.1002/0471142727.mb3101s107 · Cited by 82
Generalized bacterial genome editing using mobile group II introns and Cre‐lox
Enyeart, P. J., Chirieleison, S. M., Dao, M. N., Perůtka, J., Quandt, E. M., Yao, J., Whitt, J. T., Keatinge‐Clay, A. T., Lambowitz, A. M., & Ellington, A. D. (2013) · Molecular Systems Biology · doi:10.1038/msb.2013.41 · Cited by 83
Advancing toxicology research using in vivo high throughput toxicology with small fish models
Planchart, A. (2016) · ALTEX · doi:10.14573/altex.1601281 · Cited by 85
GenomeCRISPR - a database for high-throughput CRISPR/Cas9 screens
Rauscher, B., Heigwer, F., Breinig, M., Winter, J., & Boutros, M. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw997 · Cited by 84
Gene redundancy and gene compensation: An updated view
Peng, J. (2019) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2019.07.001 · Cited by 90
Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect
Liu, D., Wang, Z., Xiao, A., Zhang, Y., Li, W., Zu, Y., Yao, S., Lin, S., & Zhang, B. (2013) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2013.11.004 · Cited by 84
Intestinal dysmotility in a zebrafish (Danio rerio) shank3a;shank3b mutant model of autism
James, D. M., Kozol, R. A., Kajiwara, Y., Wahl, A. L., Storrs, E. C., Buxbaum, J. D., Klein, M., Moshiree, B., & Dallman, J. E. (2019) · Molecular Autism · doi:10.1186/s13229-018-0250-4 · Cited by 86
Effects of acoustic levitation on the development of zebrafish, Danio rerio, embryos
Sundvik, M., Nieminen, H. J., Salmi, A., Panula, P., & Hæggström, E. (2015) · Scientific Reports · doi:10.1038/srep13596 · Cited by 83
Genetic and neuronal regulation of sleep by neuropeptide VF
Lee, D. A., Andreev, A., Truong, T. V., Chen, A., Hill, A. J., Oikonomou, G., Pham, U., Hong, Y. K., Tran, S., Glass, L., et al. (2017) · eLife · doi:10.7554/elife.25727 · Cited by 88
Ligase IV inhibitor SCR7 enhances gene editing directed by CRISPR–Cas9 and ssODN in human cancer cells
Hu, Z. M., Shi, Z., Guo, X., Jiang, B., Wang, G., Luo, D., Chen, Y., & Zhu, Y. (2018) · Cell & Bioscience · doi:10.1186/s13578-018-0200-z · Cited by 83
Zebrafish as Toxicological model for screening and recapitulate human diseases
Caballero, M. V., & Candiracci, M. (2018) · Journal of Unexplored Medical Data · doi:10.20517/2572-8180.2017.15 · Cited by 92
Influenza A virus infection in zebrafish recapitulates mammalian infection and sensitivity to anti-influenza drug treatment
Gabor, K. A., Goody, M. F., Mowel, W. K., Breitbach, M. E., Gratacap, R. L., Witten, P. E., & Kim, C. H. (2014) · Disease Models & Mechanisms · doi:10.1242/dmm.014746 · Cited by 81
CRISPR / C as9‐mediated gene knockout in the ascidian C iona intestinalis
Sasaki, H., Yoshida, K., Hozumi, A., & Sasakura, Y. (2014) · Development Growth & Differentiation · doi:10.1111/dgd.12149 · Cited by 81
Genome editing in sea urchin embryos by using a CRISPR/Cas9 system
Lin, C.-Y., & Su, Y. (2015) · Developmental Biology · doi:10.1016/j.ydbio.2015.11.018 · Cited by 82
Development of an Efficient Genome Editing Method by CRISPR/Cas9 in a Fish Cell Line
Dehler, C. E., Boudinot, P., Martín, S., & Collet, B. (2016) · Marine Biotechnology · doi:10.1007/s10126-016-9708-6 · Cited by 86
Vertebrate-like CRYPTOCHROME 2 from monarch regulates circadian transcription via independent repression of CLOCK and BMAL1 activity
Zhang, Y., Markert, M. J., Groves, S. C., Hardin, P. E., & Merlin, C. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1702014114 · Cited by 77
A beginner's guide to understanding and implementing the genetic modification of zebrafish
Rafferty, S. A., & Quinn, T. A. (2018) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2018.07.005 · Cited by 81
Zebrafish Heart Failure Models
Narumanchi, S., Wang, H., Perttunen, S., Tikkanen, I. T., Lakkisto, P., & Paavola, J. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.662583 · Cited by 82
The tracrRNA in CRISPR Biology and Technologies
Liao, C., & Beisel, C. L. (2021) · Annual Review of Genetics · doi:10.1146/annurev-genet-071719-022559 · Cited by 91
Developing highER-throughput zebrafish screens for in-vivo CNS drug discovery
Stewart, A., Gerlai, R. T., & Kalueff, A. V. (2015) · Frontiers in Behavioral Neuroscience · doi:10.3389/fnbeh.2015.00014 · Cited by 82
Evolution of transcriptional enhancers and animal diversity
Rubinstein, M., & de Souza, F. S. J. (2013) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2013.0017 · Cited by 79
Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
Hamm, M. J., Kirchmaier, B. C., & Herzog, W. (2016) · The Journal of Cell Biology · doi:10.1083/jcb.201603100 · Cited by 82
Gene Editing With CRISPR/Cas9 RNA-Directed Nuclease
Doetschman, T., & Georgieva, T. G. (2017) · Circulation Research · doi:10.1161/circresaha.116.309727 · Cited by 91
Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo
Williams, R. M., Senanayake, U., Artibani, M., Taylor, G., Wells, D., Ahmed, A., & Sauka-Spengler, T. (2018) · Development · doi:10.1242/dev.160333 · Cited by 77
CRISPR-engineered mosaicism rapidly reveals that loss of Kcnj13 function in mice mimics human disease phenotypes
Zhong, H., Chen, Y., Li, Y., Chen, R., & Mardon, G. (2015) · Scientific Reports · doi:10.1038/srep08366 · Cited by 81
Exciting Prospects for Precise Engineering of Caenorhabditis elegans Genomes with CRISPR/Cas9
Frøkjær‐Jensen, C. (2013) · Genetics · doi:10.1534/genetics.113.156521 · Cited by 79
Preclinical Models for Alzheimer’s Disease: Past, Present, and Future Approaches
Akhtar, A., Gupta, S. M., Dwivedi, S., Kumar, D., Shaikh, M. F., & Negi, A. (2022) · ACS Omega · doi:10.1021/acsomega.2c05609 · Cited by 85
Site‐directed zebrafish transgenesis into single landing sites with the phiC31 integrase system
Mosimann, C., Puller, A., Lawson, K. L., Tschopp, P., Amsterdam, A., & Zon, L. I. (2013) · Developmental Dynamics · doi:10.1002/dvdy.23989 · Cited by 80
CRISPR/Cas9 Nuclease Cleavage Combined with Gibson Assembly for Seamless Cloning
Wang, J., Wang, A., Li, K., Wang, B., Jin, S., Reiser, M. A., & Lockey, R. F. (2015) · BioTechniques · doi:10.2144/000114261 · Cited by 80
Efficient Transcriptional Gene Repression by Type V-A CRISPR-Cpf1 from Eubacterium eligens
Kim, S. K., Kim, H., Ahn, W., Park, K., Woo, E., Lee, D., & Lee, S. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.6b00368 · Cited by 81
Genetic and epigenetic control of gene expression by CRISPR–Cas systems
Lo, A., & Qi, L. (2017) · F1000Research · doi:10.12688/f1000research.11113.1 · Cited by 72
Metabolic insights from zebrafish genetics, physiology, and chemical biology
Schlegel, A., & Gut, P. (2015) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-014-1816-8 · Cited by 84
Functions of the COPII gene paralogs SEC23A and SEC23B are interchangeable in vivo
Khoriaty, R. N., Hesketh, G. G., Bernard, A., Weyand, A. C., Mellacheruvu, D., Zhu, G., Hoenerhoff, M. J., McGee, B. M., Everett, L. A., Adams, E., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1805784115 · Cited by 82
Zebrafish as a model system for the study of hemostasis and thrombosis
Weyand, A. C., & Shavit, J. A. (2014) · Current Opinion in Hematology · doi:10.1097/moh.0000000000000075 · Cited by 81
Efficiency Optimization of CRISPR/Cas9-Mediated Targeted Mutagenesis in Grape
Ren, F., Ren, C., Zhang, Z., Duan, W., Lecourieux, D., Li, S., & Liang, Z. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00612 · Cited by 80
Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis
Enyeart, P. J., Mohr, G., Ellington, A. D., & Lambowitz, A. M. (2014) · Mobile DNA · doi:10.1186/1759-8753-5-2 · Cited by 86
Expanding Horizons: Ciliary Proteins Reach Beyond Cilia
Yuan, S., & Sun, Z. (2013) · Annual Review of Genetics · doi:10.1146/annurev-genet-111212-133243 · Cited by 81
DUSP9 Modulates DNA Hypomethylation in Female Mouse Pluripotent Stem Cells
Choi, J., Clement, K., Huebner, A. J., Webster, J. O., Rose, C. M., Brumbaugh, J., Walsh, R. M., Lee, S., Savol, A. J., Etchegaray, J., et al. (2017) · Cell stem cell · doi:10.1016/j.stem.2017.03.002 · Cited by 79
Photoperiodic and clock regulation of the vitamin A pathway in the brain mediates seasonal responsiveness in the monarch butterfly
Iiams, S. E., Lugena, A. B., Zhang, Y., Hayden, A. N., & Merlin, C. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1913915116 · Cited by 76

Authors: Hwang, W. Y., Fu, Y., Reyon, D., Maeder, M. L., Tsai, S. Q., Sander, J. D., Peterson, R. T., Yeh, J.-R. J., & Joung, J. K.
Affiliations: Massachusetts General Hospital (US)
Harvard University (US)
Broad Institute (US)
Corresponding author: Yeh, J-R Joanna
Joung, J Keith
ORCID: Hwang, Woong Y
Reyon, Deepak
Maeder, Morgan L
Tsai, Shengdar Q
Sander, Jeffry D
Peterson, Randall T
Joung, J Keith
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2013-01-29
Volume: 31
Issue: 3
Pages: 227-229
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.2501
PMC: PMC3686313
OpenAlex: W1976391205
Cited by: 2,937
References: 24
Citations by year: 2026: 68 · 2025: 125 · 2024: 146 · 2023: 159 · 2022: 207 · 2021: 203 · 2020: 227 · 2019: 230 · 2018: 252 · 2017: 246 · 2016: 306 · 2015: 276 · 2014: 300 · 2013: 189
FWCI: 160.03
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Helmholtz Centre for Ocean Research Kiel (GEOMAR) (acceptedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
Funders: National Institute on Aging (K01AG031300)
National Human Genome Research Institute (P50HG005550)
National Cancer Institute (R01CA140188)
National Institute of General Medical Sciences (DP1GM105378, R01GM088040, F32GM105189)
National Center for Chronic Disease Prevention and Health Promotion (DP1 GM105378)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
Keywords: CRISPR-Cas, genome editing, zebrafish embryos, Cas9 endonuclease, guide RNA, zinc finger nucleases, TALEN
MeSH terms: Animals, Base Sequence, DNA, Embryo, Nonmammalian, Endonucleases, Genetic Engineering, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Zebrafish, Genome, RNA Editing, DNA Cleavage, Inverted Repeat Sequences, RNA, Small Untranslated, CRISPR-Cas Systems

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Efficient genome editing in zebrafish using a CRISPR-Cas system

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks