Cited by 4,106RNA modifications and cancerEpigenetics and DNA MethylationRNA regulation and disease

The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.

N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO

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Abstract

We report here that fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m(6)A) residues in RNA in vitro. FTO knockdown with siRNA led to increased amounts of m(6)A in mRNA, whereas overexpression of FTO resulted in decreased amounts of m(6)A in human cells. We further show the partial colocalization of FTO with nuclear speckles, which supports the notion that m(6)A in nuclear RNA is a major physiological substrate of FTO.

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  1. Bokar, J.A., Shambaugh, M.E., Polayes, D., Matera, A.G. & Rottman, F.M. RNA 3, 1233–1247 (1997).

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Metadata

Authors
Jia, G., Fu, Y., Zhao, X., Dai, Q., Zheng, G., Yang, Y., Yi, C., Lindahl, T., Pan, T., Yang, Y.-G., & He, C.
Affiliations
  • University of Chicago (US)
  • Chinese Academy of Sciences (CN)
  • Cancer Research UK (GB)
Corresponding author
He, Chuan
Venue
Nature Chemical Biology
Published
2011-10-16
Volume
7
Issue
12
Pages
885-887
ISSN
  • 1552-4450 (ISSN-L)
  • 1552-4469
OpenAlex
W2071930821
Cited by
4,106
References
27
Citations by year
2026: 272 · 2025: 416 · 2024: 473 · 2023: 489 · 2022: 546 · 2021: 544 · 2020: 341 · 2019: 217 · 2018: 218 · 2017: 159 · 2016: 109 · 2015: 107 · 2014: 94 · 2013: 67 · 2012: 45
FWCI
26.98
Type
journal article
Language
en
Update notices
erratum (2012-11-26)
Open access
Locations
  • Nature Chemical Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (GM071440, R01GM071440, R01GM088599, GM088599)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
N6-methyladenosine, m6A demethylation, FTO, nuclear RNA, RNA modification, oxidative demethylation, nuclear speckles, obesity, RNA metabolism
MeSH terms
Alpha-Ketoglutarate-Dependent Dioxygenase FTO, Adenosine, Cell Nucleus, HeLa Cells, Humans, Methylation, Obesity, Oxidation-Reduction, Proteins, RNA, Messenger, Substrate Specificity

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